| Dover Lorry Park Limited | Transport & logistics | 2025 |
| Dover Marina Hotel Limited | Hospitality | 2010 |
| Dover Marine Services C.I.C. | Arts & recreation | 2011 |
| Dover Masonic Hall Company Limited(The) | Real estate | 1900 |
| Dover Operatic and Dramatic Society | Arts & recreation | 2000 |
| Dover Orion & Care Cars Limited | Transport & logistics | 2024 |
| Dover Phone Repair Centre Ltd | Other services | 2023 |
| Dover Physiotherapy Limited | Health & social care | 2014 |
| Dover Priory Hotel Limited | Hospitality | 2026 |
| Dover Rentals Ltd | Real estate | 2026 |
| Dover Retail Crime Operation | Administrative & support | 2001 |
| Dover Roofing & Brickwork Services & Supplies Ltd | Construction | 2024 |
| Dover Sea Sport Centre CIC | Arts & recreation | 2024 |
| Dover Security Ltd | Administrative & support | 2023 |
| Dover Self Storage Limited | Extraterritorial | 2000 |
| Dover Storage Ltd | Transport & logistics | 2017 |
| Dover Store Ltd | Wholesale & retail | 2023 |
| Dover Takeaway Ltd | Hospitality | 2025 |
| Dover Taxis Ltd | Transport & logistics | 2021 |
| Dover Town Commerce Limited | Wholesale & retail | 2025 |
| Dover Transport Museum Society | Arts & recreation | 1983 |
| Dover Truck Roadside Assistance Ltd | Wholesale & retail | 2015 |
| Dover Waterfront Limited | Real estate | 2015 |
| Dover, Deal & District Citizens Advice Bureau | Information & communication | 2004 |
| DOVERTAKEAWAY1 Limited | Hospitality | 2025 |
| Dovorian Limited | Water & waste | 2025 |
| Dowle Manufacturing Limited | Manufacturing | 2021 |
| Drax Metals Ltd | Extraterritorial | 2023 |
| Driani Construction Ltd | Extraterritorial | 2019 |
| Driani Toiture Invest Ltd | Professional & scientific | 2019 |
| Drivekey Corporation | | 2020 |
| Driversolution Europe Limited | Professional & scientific | 2012 |
| Drop One Services Ltd | Construction | 2002 |
| DRT Development Limited | Construction | 2018 |
| Dubris Ltd | Hospitality | 2024 |
| Dunn Virtually Ltd | Professional & scientific | 2024 |
| Dunston Rock MAN Limited | Mining & quarrying | 2026 |
| DUO Makers Corp Ltd | Wholesale & retail | 2023 |
| DW Properties and Investments Ltd | Real estate | 2024 |
| DWS 21 Ltd Limited | Transport & logistics | 2019 |
| Dynamic Business Corp Ltd | Professional & scientific | 2023 |
| Dynamic Sports Coaching Ltd | Education | 2018 |
| Dyrms - an Academy With Military Traditions | Education | 2010 |
| E & E Flaunty Ltd | Hospitality | 2019 |
| E B Taylor Limited | Professional & scientific | 1996 |
| E J Ditton & Co Limited | Construction | 2008 |
| E K Fire Protection Ltd | Other services | 2002 |
| E. Temple Holdings Ltd | Extraterritorial | 2021 |
| E.L.P Property Investments Ltd | Construction | 2026 |
| E.P. Prestige Ltd | Administrative & support | 2026 |
| E.W. GEE Limited | Construction | 2003 |
| Eagle Mount Estates Ltd | Real estate | 2025 |
| Eagles Group Limited | Wholesale & retail | 2024 |
| EAR WAX Removal by JO Perry Ltd | Health & social care | 2024 |
| Earth Clean UK Ltd | Other services | 2017 |
| Earthlings CIC | Arts & recreation | 2025 |
| East Coast Stables Ltd | Wholesale & retail | 2019 |
| East Kent Fire Protection Ltd | Other services | 2012 |
| East Kent Foods Limited | Wholesale & retail | 1992 |
| East Kent Railway Trust | Transport & logistics | 2001 |
| East Kent Recycling Limited | Construction | 2012 |
| East Kent Tippers Limited | Construction | 2023 |
| East Kent Young Performers Ltd | Arts & recreation | 2005 |
| East Sussex Endocrine Clinic Limited | Health & social care | 2022 |
| Eastling Enterprises Limited | Information & communication | 2012 |
| Eastry Civil Engineering Ltd | Construction | 2025 |
| Eastry Groundworks Ltd | Construction | 2018 |
| Eastway Escapes Limited | Administrative & support | 2025 |
| Eastwood Vehicle Transport Limited | Transport & logistics | 2021 |
| Easyskim Plastering Ltd | Construction | 2025 |
| Ebol Invest Ltd | Extraterritorial | 2021 |
| Ebol Ltd | Professional & scientific | 2021 |
| EC Air Ltd | Energy | 2018 |
| Eco Project Invest Ltd | Extraterritorial | 2022 |
| Eco Renov Invest Ltd | Extraterritorial | 2024 |
| Ecocore Facilities Ltd | Administrative & support | 2025 |
| Ecokent Ltd | Other services | 2025 |
| Ecolonergy UK Ltd | Extraterritorial | 2007 |
| Ecom Corp Ltd | Wholesale & retail | 2020 |
| Ecom Development Ltd | Extraterritorial | 2024 |
| Ecom Gold Ltd | Wholesale & retail | 2021 |
| Ecstatic Estate Limited | Real estate | 2016 |
| Edinburgh Place Management Company Limited | Extraterritorial | 1996 |
| Edmondson Hughes Ltd | Real estate | 2021 |
| Edmunds DI Ltd | Construction | 2018 |
| EE Accounts Ltd | Professional & scientific | 2018 |
| EEH GMR Properties Limited | Real estate | 2021 |
| EEX Limited | Wholesale & retail | 2014 |
| EF Pro Services Invest Ltd | Extraterritorial | 2025 |
| EF Pro Services Ltd | Extraterritorial | 2025 |
| Efesoylemez Ltd | Hospitality | 2024 |
| EKR Commercials Limited | Wholesale & retail | 2025 |
| EKR Trading Limited | Transport & logistics | 2002 |
| EL Greco 62 Invest Ltd | Extraterritorial | 2024 |
| EL Greco 62 Ltd | Extraterritorial | 2024 |
| EL Manu Vide Tout Ltd | Extraterritorial | 2025 |
| Electra-Low Ltd | Manufacturing | 2022 |
| Electrical Geodesics, Inc. | | 2013 |
| Elegantini Ltd | Other services | 2016 |
| Elektour Limited | Professional & scientific | 2020 |