| E K M Caravan Services Limited | Manufacturing | 2019 |
| E Waters Consulting Ltd | Information & communication | 2024 |
| E-Logbooks Limited | Other services | 2003 |
| E.F.Saunders & Sons Limited | Agriculture & fishing | 1960 |
| EA & JA Hiscock Limited | Agriculture & fishing | 2005 |
| Earth Tiger Associates Limited | Information & communication | 2001 |
| East Dorset Golf Club Limited | Arts & recreation | 1989 |
| East Down Consultancy Ltd | Professional & scientific | 2020 |
| East Farm Lane (Owermoigne) Ltd | Households | 2018 |
| Easterleaze Services Limited | Professional & scientific | 2021 |
| Eastgate (Dorchester) Management Company Limited | Households | 2016 |
| Eastkirk Limited | Manufacturing | 2014 |
| Eastment Windows Ltd | Construction | 2026 |
| Easton News Ltd | Wholesale & retail | 2020 |
| Eastwind Consulting Limited | Professional & scientific | 2019 |
| Easy and Clear Limited | Professional & scientific | 2021 |
| EAT in Style Limited | Hospitality | 2015 |
| Ebbstream Ltd | Professional & scientific | 2023 |
| Eclipse Technical Solutions Limited | Professional & scientific | 2014 |
| Eco Electrix Ltd | Construction | 2013 |
| Eco Metals Recovery International Limited | Manufacturing | 2019 |
| Eco Park Solutions Ltd | Manufacturing | 2021 |
| Ecommerce Boutique Ltd | Wholesale & retail | 2024 |
| ECS Interiors Limited | Information & communication | 2016 |
| ECS Special Projects Limited | Manufacturing | 2011 |
| Edgeland Limited | Hospitality | 2000 |
| Edmunds & Clarke Furniture Limited | Wholesale & retail | 2014 |
| Educated Communications Limited | Information & communication | 2018 |
| Edvita Ltd | Education | 2025 |
| Edwards and Keeping Limited | Professional & scientific | 2023 |
| EEL Electrical Ltd | Construction | 2022 |
| Effin.Co Ltd | Wholesale & retail | 2019 |
| EFG (Dorset) Limited | Professional & scientific | 2018 |
| Eicuk Limited | Hospitality | 2018 |
| Eileen Eversden Anti Coercion Limited | Real estate | 2019 |
| El-Ella Medical Services Ltd | Health & social care | 2026 |
| ELD Holdings Ltd | Financial services | 2024 |
| Eldridge, Pope & Co. (Dorset) Ltd | Manufacturing | 2025 |
| Eldridge, Pope & Company Limited | Manufacturing | 2014 |
| Elegance Weymouth Limited | Other services | 2014 |
| Elementara Ltd | Manufacturing | 2025 |
| Elephant in the Room Holdings Limited | Hospitality | 2020 |
| Elephant Introductions Ltd | Wholesale & retail | 2025 |
| Elif Food 2015 Ltd | Hospitality | 2025 |
| Elite Illuminations Ltd | Professional & scientific | 2024 |
| Elite Spas Dorset Ltd | Wholesale & retail | 2016 |
| Elixis Limited | Manufacturing | 2022 |
| Ellieswork Ltd | Health & social care | 2025 |
| Ellis James Building Services Ltd | Construction | 2019 |
| Ellis-Miller and Partners Ltd | Professional & scientific | 2025 |
| ELM (Dorchester) Ltd | Hospitality | 2022 |
| Elmtree Management Company Limited | Households | 1988 |
| Elmwood Jbgroup Ltd | Hospitality | 2026 |
| Elocin Group Ltd | Financial services | 2021 |
| ELS Accounting (SW) Ltd | Professional & scientific | 2023 |
| Emblem Property Management Ltd | Real estate | 2023 |
| Emerald Equine (International) Ltd | Wholesale & retail | 2016 |
| Employ My Ability (EMA) Limited | Education | 2014 |
| Encompass Care Organisation UK | Public administration | 1990 |
| Energise Development Limited | Administrative & support | 2020 |
| Energy Management Matters LLP | | 2009 |
| Energy Measurement Limited | Administrative & support | 2007 |
| Energy Wise Home Solutions Ltd | Construction | 2024 |
| Engcon Holdings Limited | Financial services | 2023 |
| Engineering Control Supplies Limited | Manufacturing | 1990 |
| Enka Management Company Limited | Real estate | 2009 |
| Entertainmenthouseuk Ltd | Arts & recreation | 2025 |
| Entocon Ltd | Professional & scientific | 2024 |
| EP South Coast Ltd | Hospitality | 2025 |
| Epic Print Limited | Manufacturing | 2007 |
| Epic Tales Ltd. | Information & communication | 2003 |
| Equine Rescue Services Limited | Financial services | 2008 |
| ERE Ltd | Wholesale & retail | 2023 |
| Ergoforge Ltd | Wholesale & retail | 2025 |
| Ersl Marine Ltd | Professional & scientific | 2026 |
| Escalus House Management Limited | Households | 2024 |
| ESE Lighting Direct Limited | Wholesale & retail | 2018 |
| Essex Waistcoats Limited | Manufacturing | 2006 |
| Ethel Rose Limited | Arts & recreation | 2020 |
| Etonpreneurs LLP | | 2013 |
| Euro Car and Commercial Limited | Wholesale & retail | 2025 |
| Evans Garage Ltd | Wholesale & retail | 2007 |
| EVE Line Properties Ltd | Real estate | 2024 |
| Evershot Bakery Limited | Manufacturing | 1994 |
| Evershot Farms Limited | Agriculture & fishing | 1971 |
| Evexia Home Limited | Information & communication | 2018 |
| Evoke Enterprises Limited | Extraterritorial | 2015 |
| Evoku Ltd | Information & communication | 2023 |
| Evolve Functional Training CIC | Arts & recreation | 2023 |
| Evolve Independence Ltd | Administrative & support | 2025 |
| Evolve Recruitment Ltd | Administrative & support | 2015 |
| Evolve Water Ltd | Water & waste | 2022 |
| EW Property Maintenance Service Ltd | Administrative & support | 2025 |
| Excel Asset Investments Ltd | Real estate | 2025 |
| Excel Electrical Consultants Ltd | Professional & scientific | 2017 |
| Exchange Supplies Limited | Wholesale & retail | 2002 |
| Excite Rally Raid Ltd | Arts & recreation | 2016 |
| Existing Human Ltd | Health & social care | 2023 |
| Exitjust Limited | Professional & scientific | 1991 |
| Exmouth Star Bar Ltd | Hospitality | 2025 |