| DXO Transport Ltd | Transport & logistics | 2025 |
| Dyadivine Ltd | Other services | 2022 |
| DYE & Golze Dental Laboratory Limited | Health & social care | 2008 |
| Dyja | Education | 2014 |
| Dylan Food and Drink Limited | Wholesale & retail | 2019 |
| Dynamic Heating & Interiors Ltd | Construction | 2022 |
| Dynamic Influence Limited | Professional & scientific | 2014 |
| Dynamic Property Services Limited | Construction | 2022 |
| Dynamic Services Arabia Ltd | Wholesale & retail | 2023 |
| Dynamo Luxury Detailing Ltd | Administrative & support | 2023 |
| Dynom Ltd | Information & communication | 2024 |
| Dzdeutrade Ltd | Real estate | 2023 |
| D’Spot Afro Kitchen Ltd | Hospitality | 2025 |
| E & J Flowers Limited | Wholesale & retail | 2010 |
| E a Construction (Yorkshire) Limited | Construction | 2024 |
| E a Hart Limited | Extraterritorial | 1987 |
| E Bowie Solutions Ltd | Manufacturing | 2015 |
| E C J Invest Limited | Real estate | 2021 |
| E F Forche Ltd | Construction | 2014 |
| E Force Engineering Ltd | Professional & scientific | 2026 |
| E Grant Developments Limited | Construction | 2006 |
| E H Homes Limited | Construction | 1985 |
| E L B Events Limited | Hospitality | 2026 |
| E Plus L Services Ltd | Transport & logistics | 2023 |
| E Sales Hub Limited | Administrative & support | 2021 |
| E Storey Outside Catering Limited | Hospitality | 2015 |
| E&S Produce Ltd | Hospitality | 2017 |
| E-Llume Ltd | Manufacturing | 2012 |
| E-Mule Limited | Manufacturing | 2016 |
| E-Tank Limited | Professional & scientific | 2020 |
| E-Taz Limited | Information & communication | 2007 |
| E. Claire Consultancy Limited | Professional & scientific | 2017 |
| E. Maleham (Landscaping) Limited | Administrative & support | 2004 |
| E.A.K. Group Ltd | Wholesale & retail | 2023 |
| E.Claybourn & Company Limited | Real estate | 1930 |
| E.J.S Asset Solutions Limited | Construction | 2013 |
| E.P.C.D Ltd | Real estate | 2017 |
| E.R Auto Services Ltd | Wholesale & retail | 2016 |
| E10 Studio Limited | Professional & scientific | 2018 |
| E3ANALYTICA Limited | Administrative & support | 2018 |
| E4PROPERTIES Ltd | Real estate | 2024 |
| E9 Cleaning Crew Ltd | Administrative & support | 2026 |
| EA Asset Finance Ltd | Financial services | 2022 |
| EA Builders and Decorators Ltd | Construction | 2011 |
| Eabeck Heating Solutions Ltd | Construction | 2025 |
| Each Other Care 2022 Ltd | Health & social care | 2022 |
| Each Other Care Holdings 2022 Ltd | Financial services | 2022 |
| Each Other Care Holdings Limited | Financial services | 2018 |
| Each Other Care Limited | Professional & scientific | 2018 |
| Eagle & Child ( Auckley) Limited | Hospitality | 2011 |
| Eagle Assured Roofing Ltd | Construction | 2021 |
| Eagle Medical Services Ltd | Health & social care | 2024 |
| Eagle Place Enterprise Ltd | Wholesale & retail | 2018 |
| Eaglenest Faith Ltd | Extraterritorial | 2010 |
| Eaglesvale Medical Services Ltd | Health & social care | 2025 |
| EAM Microsuction Ltd | Health & social care | 2025 |
| Eamos Investment Ltd | Real estate | 2025 |
| Earl of Doncaster Limited | Hospitality | 1995 |
| Early Years Prize Draw Limited | Administrative & support | 2021 |
| Earp Logistics Ltd | Transport & logistics | 2021 |
| Ears of Axholme Ltd | Health & social care | 2024 |
| Earth Links Ltd | Information & communication | 2008 |
| Earthchange Ltd | Extraterritorial | 2021 |
| Earthworx Limited | Construction | 2010 |
| Easap Ltd | Extraterritorial | 2016 |
| East Coast Solar Ltd | Construction | 2023 |
| East Doncaster Development Trust Limited | Administrative & support | 2003 |
| East European Market Ltd | Wholesale & retail | 2024 |
| East M Limited | Construction | 2022 |
| East Waste Limited | Water & waste | 1991 |
| Eastborough Ventures Ltd | Extraterritorial | 2026 |
| Eastern Coach Works Ltd | Wholesale & retail | 2023 |
| Eastern Sky Logistics Ltd | Transport & logistics | 2024 |
| Eastgate Holdings Ltd | Extraterritorial | 2026 |
| Eastling Kennels Limited | Agriculture & fishing | 2023 |
| Eastlound Management Ltd | Professional & scientific | 2020 |
| Eaststock Limited | Extraterritorial | 2012 |
| Easwood Joinery Limited | Construction | 2023 |
| Easy Goods Official Limited | Wholesale & retail | 2025 |
| Easy Hire Studios Ltd | Information & communication | 2025 |
| Easymods Limited | Wholesale & retail | 2024 |
| Eazzey Stores Ltd | Wholesale & retail | 2024 |
| EB Car Parts Limited | Wholesale & retail | 2025 |
| EB Homes & Property Limited | Real estate | 2026 |
| Ebas Childcare Services Ltd | Health & social care | 2013 |
| Ebeneezer Properties Limited | Real estate | 2025 |
| EBJ Developments Limited | Real estate | 2025 |
| Ebony Traffic Management Ltd | Public administration | 2025 |
| Ebor Aviation Services Limited | Transport & logistics | 2008 |
| Ebouk Limited | Wholesale & retail | 2025 |
| EC & I Contract Services Ltd | Administrative & support | 2016 |
| EC Serenity Coaching Ltd | Extraterritorial | 2022 |
| Ecclesall First AID Training Limited | Professional & scientific | 2018 |
| Echo Cart Ltd | Wholesale & retail | 2025 |
| Echocraft Communications Ltd | Extraterritorial | 2025 |
| Echoforge Developments Ltd | Extraterritorial | 2025 |
| Echolimaco Ltd | Information & communication | 2021 |
| Echoo Ltd | Information & communication | 2024 |
| Echopeak Analytics Ltd | Extraterritorial | 2026 |
| Echovale Media Ltd | Extraterritorial | 2026 |