| Automotive Utopia Ltd | Wholesale & retail | 2024 |
| AWB and SON Ltd | Public administration | 2021 |
| AWB and SON Training Limited | Information & communication | 2023 |
| Batchglow Limited | Manufacturing | 1981 |
| Besty Tasty Ltd | Hospitality | 2024 |
| Bower Power (Events) Ltd | Construction | 2014 |
| Brkhd Ltd | Extraterritorial | 2025 |
| Burpee Holdings Limited | Administrative & support | 2012 |
| Charmilian Ltd | Real estate | 2024 |
| CMM Trade Limited | Wholesale & retail | 2026 |
| Colcrete Limited | Manufacturing | 2020 |
| Coriana Enterprises Ltd | Information & communication | 2012 |
| Cybyt Limited | Information & communication | 2012 |
| DBA Building Solutions Limited | Manufacturing | 2013 |
| Dinnington Pre-School Ltd | Education | 2000 |
| Dixon Coal Ltd | Wholesale & retail | 2005 |
| Evenort Limited | Manufacturing | 1982 |
| FB Products Ltd | Manufacturing | 2018 |
| General Transport Services (Sheffield) Limited | | 1972 |
| GIX International Limited | Professional & scientific | 1995 |
| Goodysphere Ltd | Education | 2024 |
| Grimsoft Ltd | Information & communication | 2026 |
| Highfield Plant Limited | Manufacturing | 2013 |
| Hulcan Ltd | Wholesale & retail | 2019 |
| Humberside Instrument Services Ltd | Professional & scientific | 2018 |
| Indian SPICE4U Limited | Hospitality | 2018 |
| Intelliam Software Solutions Limited | Information & communication | 2024 |
| Lam-Fab Limited | Administrative & support | 1991 |
| Lindrick Holdings Limited | Professional & scientific | 2024 |
| Lindrick Landscapes Limited | | 1988 |
| LS Conversions Ltd | Other services | 2022 |
| Magnesita Refractories Limited | Administrative & support | 1992 |
| Mass Classics Ltd | Manufacturing | 2002 |
| Medivest Group Ltd | Transport & logistics | 2021 |
| Northern Groundcare Limited | Administrative & support | 1998 |
| Polymer Techniques Limited | Manufacturing | 2008 |
| PPR Knights Properties Ltd | Construction | 2024 |
| Prestige Commercial Finance Limited | Financial services | 2019 |
| Prestige Cottages & Property Management Ltd | Real estate | 2020 |
| Prestige Wills & Estate Planning Limited | Financial services | 2020 |
| Principal Couriers Ltd | Transport & logistics | 2021 |
| Probate Legal Services Ltd | Financial services | 2020 |
| Professional Training & Development Solutions Ltd. | Education | 2014 |
| R Squared Construction Ltd | Construction | 2017 |
| R Squared Group Ltd | Financial services | 2025 |
| Riverside Precision Materials Limited | Mining & quarrying | 2021 |
| Rock Lettings Ltd | Real estate | 2024 |
| Satstore Ltd | Wholesale & retail | 2023 |
| Sherwood Corporate Solutions Limited | Financial services | 2002 |
| Sliptec Limited | Manufacturing | 2018 |
| Spectre Technical Services Ltd | Construction | 2024 |
| Staysafe M&E Limited | Construction | 2017 |
| T.H.E. Subsid Limited | Extraterritorial | 1991 |
| THE Boot Lady Ltd | Wholesale & retail | 2025 |
| THE Northern Elephant Ltd | Hospitality | 2014 |
| Ttrentco Ltd | Real estate | 2026 |
| Vospal Limited | Information & communication | 2015 |
| Westgate Developments (Worksop) Limited | Construction | 2012 |
| Wingman Drones Ltd | Information & communication | 2024 |