| DOT NET Insight Ltd | Information & communication | 2011 |
| Double Decker Music Ltd | Information & communication | 2018 |
| Dovetail Creative Limited | Information & communication | 2022 |
| Downland Dance Limited | Education | 2017 |
| DR. Bionutrition Ltd | Manufacturing | 2023 |
| Dragon E&E Ventures Limited | Real estate | 2024 |
| Dragonfly Stays Limited | Real estate | 2025 |
| Draycott Engineering Limited | Wholesale & retail | 2017 |
| Dreamwood Property Limited | Households | 2021 |
| Dreyer Investments Limited | Real estate | 2025 |
| Drinks & Nibbles Ltd | Wholesale & retail | 2025 |
| Driveforce Pro Recruitment Ltd | Administrative & support | 2024 |
| Drone Motion Ltd | Information & communication | 2026 |
| DRP Touring Ltd | Arts & recreation | 2015 |
| Drywallers Ltd | Construction | 2017 |
| DSA Trade Ltd | Wholesale & retail | 2025 |
| Dssech Ltd | Financial services | 2025 |
| Dtgl Dorothy Thompson Grasmere Limited | Real estate | 2025 |
| DUN Ltd | Construction | 2016 |
| DUO Services Ltd | Information & communication | 2020 |
| Dust and Dazzle Ltd | Administrative & support | 2023 |
| Dutch N' Such Ltd | Hospitality | 2020 |
| Dynasty Windows, Doors & Glazing Systems Ltd | Construction | 2024 |
| D’Arcy Enterprises Limited | Real estate | 2025 |
| E & F Miracle Cleaners Ltd | Construction | 2025 |
| E a Recovery & Contracting Services Limited | Wholesale & retail | 2000 |
| E and W Plumbing and Heating Ltd. | Construction | 2014 |
| E&M Management Ltd | Professional & scientific | 2019 |
| Easidisplays Limited | Manufacturing | 2026 |
| East Hagbourne Community Shop Limited | Wholesale & retail | 2003 |
| East Midland Baptist Trust Company Limited | Administrative & support | 1921 |
| Easy Motors Ltd | Wholesale & retail | 2019 |
| Easy Squeaks Ltd | Administrative & support | 2024 |
| Ebmtopco Ltd | Financial services | 2023 |
| Ecat Consulting Limited | Professional & scientific | 2026 |
| Echelon Energy Limited | Construction | 2025 |
| Eckert & Ziegler Environmental Services Limited | Professional & scientific | 2013 |
| Eclipse Energy Ltd | Professional & scientific | 2022 |
| Eco Executive Cars Ltd | Transport & logistics | 2024 |
| Ecom Hustle Ltd | Professional & scientific | 2022 |
| Edbraids Hair and Beauty Cosmetics Ltd | Health & social care | 2025 |
| Eden Properties (Oxford) Ltd | Real estate | 2025 |
| Edexe Limited | Information & communication | 2009 |
| Edmil Accountants (UK) Ltd | Professional & scientific | 2016 |
| Edmil Lettings and Management Ltd | Real estate | 2023 |
| Edmil Umbrella Company Ltd | Financial services | 2019 |
| Ednewton Consulting Ltd | Professional & scientific | 2024 |
| Edwards Ecology & Environment Limited | Professional & scientific | 2016 |
| EE Services Ltd | Manufacturing | 2020 |
| Eebn Ltd | Real estate | 2025 |
| Effendy Ventures Ltd | Hospitality | 2024 |
| EGA Logistics Ltd | Wholesale & retail | 2020 |
| Eldridge Environmental Services Ltd | Professional & scientific | 2025 |
| Electrical & Instrument Solutions Ltd | Manufacturing | 2017 |
| Electrical Contractor Services Ltd | Construction | 2025 |
| Element SIX (UK) Limited. | Professional & scientific | 1972 |
| Element SIX Limited | Professional & scientific | 1994 |
| Element SIX Technologies Limited | Manufacturing | 2012 |
| Elevatex Ltd | Information & communication | 2025 |
| Elite EOD Solutions Ltd | Public administration | 2024 |
| Elite Youth Sports Ltd | Education | 2020 |
| Elizu Services Ltd | Wholesale & retail | 2016 |
| Ellis Professional Paramedic Services Limited | Public administration | 2025 |
| Elrise Limited | Real estate | 2016 |
| EM Beauty Forever Ltd | Other services | 2016 |
| Embed Learning & Clear Training Ltd | Professional & scientific | 2010 |
| Emberline Group Ltd | Construction | 2026 |
| Emendation Consulting Limited | Professional & scientific | 2022 |
| Emerald Tpds Ltd | Professional & scientific | 2022 |
| Emfs Homes Limited | Households | 2020 |
| Emily L Roberts Strategic Marketing Consultancy Ltd | Professional & scientific | 2026 |
| Eminel Ltd | Wholesale & retail | 2018 |
| Emirafix Ltd | Professional & scientific | 2015 |
| Emma's Vintage Style Ltd | Wholesale & retail | 2019 |
| Emmanuelle Tutoring Establishment Ltd | Education | 2025 |
| Empower Investing Ltd | Financial services | 2025 |
| Empowered Assets Ltd | Real estate | 2018 |
| Endeavor Engineering Ltd | Professional & scientific | 2012 |
| Enduro Global Ltd | Wholesale & retail | 2023 |
| ENGCEPT3D Ltd | Professional & scientific | 2020 |
| Eniro Care Ltd | Health & social care | 2021 |
| Eniro Investments Ltd | Real estate | 2025 |
| Ensign Chauffeurs Ltd | Transport & logistics | 2024 |
| Ensiq Ltd | Professional & scientific | 2026 |
| Entecna Ltd | Professional & scientific | 2021 |
| Entrevia Ltd | Other services | 2025 |
| Envirotec Estates Ltd | Real estate | 2022 |
| EOS Space Technology Ltd | Manufacturing | 2024 |
| Eosphere Limited | Information & communication | 2001 |
| Eporium Ltd | Wholesale & retail | 2022 |
| Epsilon Advisory Limited | Professional & scientific | 2024 |
| Equality Property Services Ltd | Construction | 2024 |
| Equation Audiovisual Limited | Other services | 1999 |
| Erems & Sons Limited | Real estate | 2025 |
| Eric Estates Ltd | Real estate | 2024 |
| Ernies Property Services Ltd | Households | 2023 |
| Eros Consulting Group Ltd | Professional & scientific | 2023 |
| Esjay Properties Ltd | Construction | 2024 |
| Essentiality Ltd | Extraterritorial | 2013 |
| ET4U Ltd | Extraterritorial | 2014 |