| 13TH Unit Ltd | Information & communication | 2026 |
| 1971 Enterprises Ltd | Hospitality | 2025 |
| 360 Medline Limited | Health & social care | 2023 |
| A a K Haulage Limited | Transport & logistics | 2002 |
| A-S Sattar C/Store Ltd | Wholesale & retail | 2025 |
| ABL Fabrications Welding Engineers Ltd | Manufacturing | 2014 |
| Acrux Pharmaceutical Limited | Wholesale & retail | 2025 |
| ACT Theatre School Limited | Arts & recreation | 2025 |
| ADS Cleaning Services Limited | Administrative & support | 2025 |
| Afterglow Beauty Denny Ltd | Other services | 2024 |
| AGW Property Ltd | Real estate | 2023 |
| Alan Wilson Joiners & Builders Ltd | Construction | 2019 |
| Aleeha's Ltd | Wholesale & retail | 2025 |
| Allan Turnbull Limited | Wholesale & retail | 2017 |
| Alliance Motorsport Solutions Ltd | Wholesale & retail | 2025 |
| Alpa Joiners Limited | Construction | 2000 |
| AMC Safety Management Ltd | Professional & scientific | 2014 |
| Apex Vets Limited | Professional & scientific | 2009 |
| Arpc (Scotland) Limited | Construction | 2016 |
| Aspen Medical Aesthetics Ltd | Health & social care | 2024 |
| Auchenhill Farms Ltd | Construction | 2017 |
| Avanis Energy Ltd | Energy | 2026 |
| Avanis Online Ltd | Wholesale & retail | 2023 |
| Ayub ND Sons Ltd | Hospitality | 2025 |
| Barleyfield Developments Limited | Construction | 2015 |
| Barry Macmillan Joinery Ltd. | Construction | 2011 |
| Beechtree Wright Limited | Construction | 2019 |
| Bennie Electrical Limited | Construction | 2020 |
| BIO Retail Solutions Limited | Wholesale & retail | 2025 |
| BJS Property Solutions Ltd | Real estate | 2019 |
| Boghead Properties Ltd | Real estate | 2020 |
| Bonnie Lets Ltd | Real estate | 2026 |
| Booths R US Ltd | Professional & scientific | 2025 |
| Bothwell Land & Development Ltd | Construction | 2021 |
| BPM Environmental Ltd | Professional & scientific | 2025 |
| Browns for Beds Limited | Wholesale & retail | 2018 |
| Bryans Coaches Limited | Transport & logistics | 2005 |
| Buildvale Limited | Construction | 2008 |
| Cairns Connects Ltd | Hospitality | 2019 |
| Carron Valley Properties Ltd | Real estate | 2025 |
| Carter Operations Ltd | Water & waste | 2025 |
| Cathan Dann Enterprise Ltd | Wholesale & retail | 2025 |
| Central Belt Masonry Ltd | Construction | 2023 |
| Central Cleaning Services Limited | Administrative & support | 2008 |
| Central Cooling Systems (Scotland) Ltd. | Manufacturing | 1998 |
| CF Scotland Ltd | Professional & scientific | 2012 |
| Claire Bennett Ltd. | Other services | 2002 |
| Clan Mcpipe Ltd | Manufacturing | 2025 |
| Cleaning Company Central Scotland Limited | Administrative & support | 2024 |
| Clement Painting and Decorating Limited | Extraterritorial | 2022 |
| CM Autosport Ltd | Wholesale & retail | 2024 |
| CM Construction (Denny) Ltd | Construction | 2025 |
| CMD Scotland Limited | Construction | 2019 |
| Cochrane Management Services Ltd | Professional & scientific | 2019 |
| Combat International Ltd | Administrative & support | 2022 |
| Convenience Store One Ltd | Wholesale & retail | 2026 |
| Coyle Consultancy Ltd | Professional & scientific | 2023 |
| Craig Wynne Joinery Limited | Construction | 2022 |
| Creative Homecare CIC | Health & social care | 2019 |
| Curran Fitness Ltd | Arts & recreation | 2025 |
| D D Interiors Limited | Wholesale & retail | 2024 |
| D R Joinery & Home Improvements Limited | Construction | 2019 |
| D.A.C Civils Ltd | Construction | 2022 |
| D.G.Garden Services Ltd | Construction | 2020 |
| Dalriada Consultancy Services Ltd | Professional & scientific | 2019 |
| Dancetek Ltd | Arts & recreation | 2023 |
| Deesgee Ltd | Hospitality | 2017 |
| Delta Integrated Solutions Ltd | Wholesale & retail | 2017 |
| Denham Plumbing & Heating Ltd | Construction | 2025 |
| Denny Optical Ltd | Wholesale & retail | 2022 |
| Denny Retail Ltd | Wholesale & retail | 2025 |
| Denny Takeaway Ltd | Hospitality | 2023 |
| DENNY30FOOD Ltd | Hospitality | 2024 |
| Denovan Village Limited | Hospitality | 2015 |
| Deuchar Williams Health (Carse) Ltd | Health & social care | 2025 |
| Deuchar Williams Health (Coll) Limited | Health & social care | 2025 |
| Deuchar Williams Health (East Fife) Ltd | Health & social care | 2025 |
| Dicarlosproperties Ltd | Real estate | 2020 |
| Dicarlospropertymanagement Ltd | Hospitality | 2023 |
| Dicksons Plumbing and Heating Ltd | Construction | 2013 |
| Diesel Direct (Scotland) Ltd | Manufacturing | 2007 |
| DJS Bodyshop Ltd | Wholesale & retail | 2025 |
| DKA Properties Ltd | Real estate | 2019 |
| Dornoch Holdings Ltd | Professional & scientific | 2015 |
| Drum Farm Hideaways Ltd | Hospitality | 2024 |
| Dryburgh Avenue Limited | Wholesale & retail | 2024 |
| Dunavon Properties Ltd | Real estate | 2025 |
| Duncan Mechanical Services Ltd | Manufacturing | 2019 |
| Duncan Specialist Welding Ltd | Manufacturing | 2007 |
| Duncarron Fort Limited | Construction | 2004 |
| Dylan Mccafferty Ltd | Construction | 2025 |
| E21 Engineering Ltd | Professional & scientific | 2024 |
| Eco RC Ltd | Construction | 2025 |
| Ecossystems Limited | Professional & scientific | 2004 |
| Edinburgh Airport Chauffeurs Ltd | Transport & logistics | 2025 |
| Eillah Plumbing & Heating Ltd | Construction | 2024 |
| Ekammam Limited | Wholesale & retail | 2023 |
| Elco Solutions Limited | Construction | 2016 |
| Electrical Testing Services Limited | Construction | 2016 |
| Elite Evolution Cheer and Dance CIC | Other services | 2019 |