| A M Phillips Home Improvement Services Ltd | Construction | 2016 |
| A Pridmore Window Hygienist Limited | Administrative & support | 2003 |
| A.Berry Support Services Limited | Health & social care | 2016 |
| Aptus Fastener Systems Ltd | Wholesale & retail | 1992 |
| Asquith Hart Limited | Financial services | 2013 |
| Atlas Janitorial & Catering Supplies (UK) Limited | Wholesale & retail | 2007 |
| Avantek Machinery Limited | Manufacturing | 2014 |
| Axess Systems Ltd | Information & communication | 2000 |
| BE Engineering Ltd | Administrative & support | 2017 |
| Brett Smith Electrical Contractors Limited | Construction | 2012 |
| Bright Fame Holding UK Limited | Administrative & support | 2016 |
| Browns (Littleover ) Ltd | Wholesale & retail | 2015 |
| BUD Gremlin Ltd | Other services | 2019 |
| C Prince Financial Services Limited | Financial services | 2006 |
| Callexa Limited | Other services | 2011 |
| Campy's Construction (Derby) Ltd | Construction | 2025 |
| Carcraft Bodyshop Limited | Manufacturing | 2019 |
| CJS Decorating (Derby) Ltd | Construction | 2010 |
| Clear and Fresh Window Repairs Limited | Construction | 2003 |
| Clive Paulson Plumbing Limited | Construction | 2012 |
| Cockapoo World Ltd | Agriculture & fishing | 2026 |
| Coldwall Technology Solutions Ltd | Other services | 2013 |
| Corner Carpets (Midlands) Limited | Construction | 2013 |
| Crown Armouries Limited | Wholesale & retail | 2012 |
| CX Automotive Limited | Wholesale & retail | 2006 |
| D J Earnshaw Ltd | Wholesale & retail | 2007 |
| D Lane Construction Limited | Construction | 2008 |
| D T Green Joinery Limited | Construction | 2006 |
| D.J. Fire Protection Services Ltd | Public administration | 2009 |
| DA Fleming Ltd | Construction | 2018 |
| Definitive Financial Services Limited | Financial services | 2007 |
| Denby Construction (East Midlands) Limited | Administrative & support | 2014 |
| Denby UK Nominees Limited | Professional & scientific | 2017 |
| Derby Plumbing Services Limited | Construction | 2004 |
| Derby Signs Limited | Extraterritorial | 2010 |
| Derbyshire Test Centres Limited | Wholesale & retail | 2006 |
| Derwent Packaging Limited | Manufacturing | 1994 |
| Derwent Print Group Limited | Financial services | 2023 |
| DKP Aquatics Ltd | Wholesale & retail | 2019 |
| Drive in Golf Ltd | Arts & recreation | 2025 |
| Duffield Hair Design Limited | Other services | 2005 |
| E - Web Developments Limited | Information & communication | 2003 |
| E.H. Caswell Limited | Wholesale & retail | 2005 |
| Ellis Smith Financial Services Ltd | Professional & scientific | 2017 |
| Estate Maintenance Services (Midlands) Limited | Construction | 2010 |
| Fladbury Pies & Sausages Limited | Wholesale & retail | 2005 |
| Fleet & Sons Plumbing & Heating Limited | Construction | 2010 |
| Foster Electrical (Ilkeston) Limited | Construction | 2004 |
| Furness Electrical Ltd | Construction | 2015 |
| G C Commercial Diving Ltd | Arts & recreation | 2017 |
| Gary Adul Limited | Construction | 2014 |
| Golden Gate (Belper) Takeaway Limited | Hospitality | 2009 |
| GR4FX Ltd | Information & communication | 2017 |
| Greengate Business Solutions Limited | Professional & scientific | 2008 |
| Guinot by SUE Ltd | Other services | 2024 |
| Hairport Hairdressing (Midlands) Ltd | Other services | 2017 |
| Happy Accident Productions Limited | Information & communication | 2010 |
| Hayes Financial Services Limited | Financial services | 2006 |
| Henderson Productions Limited | Manufacturing | 2014 |
| Hitchings Productions Limited | Information & communication | 2011 |
| HJB Limited | Wholesale & retail | 2007 |
| HMF Hair Company Limited | Other services | 2007 |
| Hulwar Automation Limited | Professional & scientific | 2005 |
| I Duddy Joinery Limited | Construction | 2007 |
| J Ball GAS Services Limited | Construction | 2013 |
| J C Construction (Derby) Limited | Construction | 2013 |
| J G S Construction Services Limited | Construction | 2007 |
| J P Building Services (Derby) Limited | Construction | 2004 |
| J Shaw Financial Services Limited | Financial services | 2006 |
| Jasper Acquisition Holdings Limited | Professional & scientific | 2007 |
| JB Estates East Midlands Ltd | Construction | 2018 |
| Jim's Joinery Limited | Construction | 2014 |
| JNH Nursing Consultancy Ltd | Health & social care | 2017 |
| John Benfield HR Consultancy Limited | Administrative & support | 2010 |
| John Mattison (Carpet Fitting) Limited | Construction | 2003 |
| Jolly Sailor (Hemington) Limited | Hospitality | 2016 |
| JPD Plastering Limited | Construction | 2008 |
| JR Pipework Services Ltd | Construction | 2010 |
| Keogh - Pipes Development Ltd | Construction | 2015 |
| Lean Methods Consulting Ltd | Professional & scientific | 2016 |
| Liniar Limited | Manufacturing | 1997 |
| LMP 2025 Ltd | Construction | 2025 |
| LMP Electrical (Midlands) Ltd | Construction | 2018 |
| Logs (Belper) Limited | Agriculture & fishing | 2013 |
| MAB Engineering Ltd | Manufacturing | 2020 |
| Mark Williamson & SON Plumbing Limited | Construction | 2011 |
| Masteroll Products Limited | Hospitality | 1995 |
| Nigel James Consulting Limited | Financial services | 2014 |
| North Coast Armouries Ltd | Arts & recreation | 2009 |
| Oak Green Design and Construct Ltd | Construction | 2017 |
| Octroi Group Limited | Professional & scientific | 1975 |
| P & T Property Solutions Ltd | Households | 2016 |
| Park Hall Garage (Ripley) Ltd | Wholesale & retail | 2009 |
| Paul Braley Commercial Valeting Limited | Other services | 2012 |
| Q & a Structures Limited | Construction | 2015 |
| Quanex Extruded Solutions Ltd | Professional & scientific | 1993 |
| Quanex Management Limited | Professional & scientific | 1999 |
| Quantum Engineers Limited | Extraterritorial | 1994 |
| R & R Plumbing & Heating (Derby) Ltd | Construction | 2015 |
| R J Peat Building Limited | Construction | 2005 |