| Driven FS Ltd | Professional & scientific | 2024 |
| DSE Waterland Ltd | Construction | 2007 |
| DSI Chester Ltd | Construction | 2024 |
| DT Chemicals Ltd | Wholesale & retail | 2009 |
| DTS Garage Services Ltd | Wholesale & retail | 2021 |
| Duas 1 Ltd | Transport & logistics | 2025 |
| Dudu and Maya Limited | Construction | 2025 |
| Duffy's Engineering Services Ltd | Professional & scientific | 2025 |
| Durable Cast Parts Limited | Manufacturing | 2002 |
| DUT DOT DEV Ltd | Information & communication | 2026 |
| Duttons Landscaping Ltd | Construction | 2017 |
| Duxbury Chauffeur Drive Ltd | Transport & logistics | 2026 |
| DVW Transport Ltd | Transport & logistics | 2021 |
| E & a Hammond Ltd | Wholesale & retail | 2011 |
| E. Stuart Jones (Dobshill Garage) Limited | Wholesale & retail | 1978 |
| Earth Sand Fire Ltd | Arts & recreation | 2024 |
| East Car Export Limited | Wholesale & retail | 2019 |
| Eastgate Advisory Limited | Information & communication | 2023 |
| Easyserve Ltd | Professional & scientific | 2023 |
| Eatilo Pearce Limited | Wholesale & retail | 2025 |
| Ebrill CYF | Real estate | 2021 |
| Eco Elegance Cleaning by Matty & Charlotte Ltd | Administrative & support | 2024 |
| Edge Transport (Properties) Limited | Transport & logistics | 2014 |
| Edge Transport Limited | Transport & logistics | 1985 |
| Edwards Homes (Bevan Mews) Ltd | Construction | 2025 |
| Edwards Trading Limited | Wholesale & retail | 2020 |
| Edwin C. Farrall (Transport) Limited | Transport & logistics | 1972 |
| EE & TP Limited | Real estate | 2012 |
| Egreen Vpower Insight Ltd | Construction | 2025 |
| EH Planning Solutions Limited | Professional & scientific | 2022 |
| Ehbuilding Limited | Construction | 2018 |
| EIGHTY4 Investment Group Limited | Real estate | 2021 |
| Einkorn Ltd | Hospitality | 2025 |
| Eirelume Ltd | Professional & scientific | 2025 |
| Elate Investments Ltd | Real estate | 2018 |
| Electric Lift Trucks Limited | Construction | 2017 |
| Electroimpact UK Limited | Manufacturing | 2000 |
| Elfen Newydd Limited | Financial services | 2019 |
| Elisa Happy Living Limited | Real estate | 2020 |
| Ellayco Ltd | Transport & logistics | 2026 |
| Ellis-Granger Limited | Real estate | 2025 |
| ELM Gable Construction Ltd | Construction | 2017 |
| Elms Court Management (ST. Annes) Limited | Real estate | 2009 |
| Elos Properties Ltd | Real estate | 2024 |
| Elvaldic Ltd | Real estate | 2024 |
| Emdells Holdings Ltd | Financial services | 2019 |
| Emdells Limited | Real estate | 2002 |
| Emeai Limited | Professional & scientific | 2018 |
| Emilie & M Properties Ltd Ltd | Real estate | 2025 |
| Empire Hair, Beauty & Extensions Limited | Other services | 2020 |
| Empres Ltd | Professional & scientific | 2011 |
| Emsocial Work Ltd | Health & social care | 2025 |
| Encapture Limited | Energy | 2011 |
| Endurmeta Ltd | Water & waste | 2009 |
| Enturi Energy Ltd | Professional & scientific | 2022 |
| Enturi Solutions Ltd | Professional & scientific | 2021 |
| Enviromix (Chester) Ltd | Construction | 2018 |
| EP17 Ltd | Wholesale & retail | 2024 |
| Eqhorsewear Ltd | Wholesale & retail | 2025 |
| Equestria Ltd | Financial services | 2025 |
| Equipping the Church Limited | Wholesale & retail | 2020 |
| Erdogan Estates Ltd | Real estate | 2024 |
| Ergonology Ltd | Information & communication | 2003 |
| Ertiol Limited | Real estate | 2025 |
| ESB Management Ltd | Extraterritorial | 2015 |
| ESK 63 Limited | Hospitality | 2024 |
| ESK Food Ltd | Hospitality | 2021 |
| Ethikos Capital Limited | Financial services | 2025 |
| Ethos Eco Epse Limited | Professional & scientific | 2024 |
| European Ventures & Acquisitions Limited | Administrative & support | 2015 |
| Ewloe Hand Car Wash Ltd | Other services | 2023 |
| Exodat Data Systems Limited | Information & communication | 2010 |
| Express Digital Limited | Information & communication | 2020 |
| F & Y Agri Limited | Agriculture & fishing | 2014 |
| F Blair Limited | Wholesale & retail | 2013 |
| F&F Travel Ltd | Administrative & support | 2024 |
| F.G.Walker Limited | Wholesale & retail | 1964 |
| Fairhurst NW Ltd | Construction | 2017 |
| Fairpark Care Limited | Health & social care | 2021 |
| Family Kardesler Ltd | Hospitality | 2025 |
| Family Transport Ltd | Transport & logistics | 2022 |
| Fanportal Limited | Wholesale & retail | 2025 |
| Farnhurst Medical Limited | Health & social care | 1993 |
| Farralls Transport (Properties) Limited | Transport & logistics | 2014 |
| Farringtons Holdings Limited | Professional & scientific | 2008 |
| Farringtons Limited | Agriculture & fishing | 1999 |
| Fast Fit Trade Supplies Ltd | Wholesale & retail | 2008 |
| Fatima Construction Group Ltd | Manufacturing | 2024 |
| Fauset Ltd | Real estate | 2026 |
| FAY VON 328 Limited | Hospitality | 2026 |
| FCC Holdco Limited | Financial services | 2024 |
| Federal Capital Limited | Financial services | 2012 |
| Ferry Chem Limited | Wholesale & retail | 1993 |
| Ferry Community Garden CIC | Other services | 2022 |
| Field Bros Ltd | Extraterritorial | 2014 |
| Finnie Rope Access Ltd | Construction | 2026 |
| Fire Prevention and Protection Ltd | Public administration | 2024 |
| Fireprotect (Chester) Limited | Manufacturing | 1981 |
| First Choice Catering Group Ltd | Hospitality | 2026 |
| Flame Homeware Limited | Wholesale & retail | 1996 |