| Modular Home Build Ltd | Manufacturing | 2025 |
| Modupe Adenigba Consulting Limited | Information & communication | 2025 |
| Mojo School Ltd | Information & communication | 2025 |
| Moloney & Moss Limited | Wholesale & retail | 2025 |
| Morgan Hair Studio Limited | Other services | 2008 |
| Motion Industrial Products Limited | Administrative & support | 2009 |
| Mount Street Advisory Limited | Professional & scientific | 2024 |
| MPC Aviation Limited | Professional & scientific | 2019 |
| MPC Land Based Ltd | Other services | 2024 |
| MTS Legal Limited | Professional & scientific | 2013 |
| Muddy Waters Limited | Agriculture & fishing | 2025 |
| Munda Ltd | Health & social care | 2025 |
| MVA Chester Limited | Administrative & support | 2002 |
| MW Building & Joinery Ltd | Construction | 2026 |
| My Hair Lady Ltd | Other services | 2015 |
| MZ Cleaning Limited | Other services | 2019 |
| N & L Property Rentals Ltd | Real estate | 2023 |
| N2 Associates Limited | Professional & scientific | 2018 |
| Nandz Ltd | Information & communication | 2020 |
| National Industries Limited | Education | 2026 |
| Navigate Group Ltd | Administrative & support | 2022 |
| NC Engineering Management Ltd | Professional & scientific | 2025 |
| NDT Group Holding Company Limited | Professional & scientific | 2023 |
| NDT Group Ltd | Professional & scientific | 2017 |
| NDT Innovation Ltd | Professional & scientific | 2023 |
| NDT+ Ltd | Professional & scientific | 2019 |
| Needle Industries (Sheffield) Limited | Professional & scientific | 1904 |
| Neil Jeckell Mechanical Design Ltd | Professional & scientific | 2026 |
| Nelltab Ltd | Information & communication | 2014 |
| Nestora Consulting Limited | Administrative & support | 2023 |
| Netdash Solutions Limited | Other services | 2024 |
| New Concepts Limited | Other services | 2002 |
| Newhive Cleans Ltd | Administrative & support | 2020 |
| Newkeeco Limited | Wholesale & retail | 2016 |
| Newton and Bell Associates Limited | Financial services | 2015 |
| Newton and Bell Limited | Other services | 2012 |
| Newydd Catering & Cleaning Limited | Hospitality | 2017 |
| Nexus Legal Ltd | Professional & scientific | 2013 |
| Nexus Supply Group Ltd | Wholesale & retail | 2022 |
| NFM Services Ltd | Transport & logistics | 2021 |
| NGP Developments North West Ltd | Construction | 2024 |
| Nicholas & Bennett Ltd | Hospitality | 2025 |
| Nick Auto Detailing Limited | Wholesale & retail | 2024 |
| Nlighten Consulting Limited | Other services | 2024 |
| Nlighten Holdings Limited | Financial services | 2025 |
| NLJ Contracting Limited | Professional & scientific | 2013 |
| Nobasicprep Ltd | Hospitality | 2023 |
| Nojen Clinical Limited | Wholesale & retail | 2025 |
| Northern Number Plates Limited | Wholesale & retail | 2017 |
| Northgene Limited | Health & social care | 1994 |
| Northwest Transport Solutions Limited | Professional & scientific | 2011 |
| Nottingham Medical Equipment Limited | Professional & scientific | 1946 |
| Novacare UK Limited | Health & social care | 1996 |
| NR Building Limited | Construction | 2011 |
| NR Shellfish Ltd | Agriculture & fishing | 2023 |
| NRF Services Ltd | Administrative & support | 2023 |
| O&S Electronic Services Ltd | Other services | 2010 |
| O-Mega Smart Ltd | Professional & scientific | 2019 |
| Oakbrook Property Holdings Limited | Real estate | 2026 |
| Oakley Grange Open Space Management Ltd | Households | 2022 |
| Oakmont Wellness Group Ltd | Other services | 2026 |
| Oaksfield Consultancy Ltd | Construction | 2024 |
| Oblivion Systems Ltd | Information & communication | 2026 |
| Ocean Tanning Ltd | Arts & recreation | 2004 |
| OE Creative Ltd | Information & communication | 2021 |
| Office Furniture Place Chester Limited | Wholesale & retail | 2010 |
| Ohana Strengthening Families Ltd | Health & social care | 2019 |
| Omanta Studios Limited | | 1991 |
| Omega A.S. Ltd | Professional & scientific | 2024 |
| Only 9 Limited | Wholesale & retail | 2018 |
| Optimiz Ltd | Information & communication | 2025 |
| Opulentia Capital Ltd | Administrative & support | 2015 |
| ORB Observation Ltd | Information & communication | 2014 |
| Orchid Flooring Holdings Limited | Administrative & support | 2024 |
| Organic Plant Foods Limited | Wholesale & retail | 2014 |
| Oriental Star 8 Ltd | Hospitality | 2026 |
| Orna Holdings Limited | Financial services | 2023 |
| Orouast Ltd | Professional & scientific | 2026 |
| Osprey Television Limited | Information & communication | 2020 |
| Outlanda Events Limited | Administrative & support | 2018 |
| Owen Promotions Limited | Arts & recreation | 1998 |
| P and B Trading Ltd | Construction | 2025 |
| P J H Building Solutions (North West) Ltd | Construction | 2020 |
| P J Processing Ltd | Wholesale & retail | 2008 |
| P R Edwards Limited | Health & social care | 2015 |
| P R T Inspection Ltd | Professional & scientific | 2013 |
| P&C Cummings Ltd | Real estate | 2024 |
| Pamela Clarke Consulting Limited | Information & communication | 2006 |
| Parapromotions Limited | Administrative & support | 2005 |
| Parkgate Construction Services (NW) Ltd | Construction | 2024 |
| Parry Property Investments Ltd | Real estate | 2020 |
| Party Wall Surveyors North Wales Ltd | Construction | 2012 |
| Pathwing Ltd | Other services | 2024 |
| Patrick Corbett Limited | Administrative & support | 2024 |
| Paul Duncan Limited | Other services | 2024 |
| Paul Hawkins Haulage Limited | Transport & logistics | 2002 |
| Paul Ronald Investments Limited | Other services | 2011 |
| Pavlou Plumbers Ltd | Other services | 2014 |
| Paws for Conservation Limited | Other services | 2023 |
| Pawsome Walks Chester Ltd | Other services | 2026 |