| 360 Property Investment Limited | Extraterritorial | 2017 |
| A M SIG Limited | Professional & scientific | 2023 |
| A&L Assets Ltd | Wholesale & retail | 2021 |
| A.M Steel Projects Ltd | Construction | 2026 |
| Active Mechanical Services Limited | Extraterritorial | 2008 |
| Agri-Services Limited | Administrative & support | 1999 |
| AM Guthrie Ltd | Professional & scientific | 2022 |
| Anderson Quantum Services Ltd | Professional & scientific | 2019 |
| Atlantico Procurement Ltd | Wholesale & retail | 2022 |
| Audio Gluttony Limited | Manufacturing | 2025 |
| Avenue Kids Ltd | Wholesale & retail | 2019 |
| Ayrshire Commercial Spares Limited | Wholesale & retail | 2012 |
| Ayrshire Fast Food Ltd | Hospitality | 2024 |
| Beraka Ltd | Financial services | 2022 |
| Best Ever Kurdish Barber Ltd | Other services | 2023 |
| Big Hand Events Ltd | Professional & scientific | 2017 |
| Bill Lorimer Ltd | Construction | 2015 |
| Braidley Energy Limited | Energy | 2022 |
| Calmar Roofing Limited | Construction | 2020 |
| Chesnutt Skeoch Limited | Real estate | 1993 |
| Craws Ltd | Professional & scientific | 2012 |
| Cygnet Solutions Limited | Information & communication | 1995 |
| D2C2 Limited | Manufacturing | 2017 |
| Darvel 1889 Trading Ltd | Hospitality | 2025 |
| Darvel Fast Food Ltd | Hospitality | 2025 |
| Darvel FC 1889 C.I.C. | Arts & recreation | 2025 |
| Darvel Improvement Group | Other services | 2002 |
| Darvel Music Company | Arts & recreation | 2008 |
| Darvel Singh Group Ltd | Wholesale & retail | 2024 |
| Darvel Storage Solutions Ltd | Transport & logistics | 2025 |
| Darvel Takeaway Ltd | Hospitality | 2013 |
| David Service 1965 Limited | Extraterritorial | 2013 |
| Dylchaada Investments Limited | Real estate | 2021 |
| East 1966 Limited | Extraterritorial | 2013 |
| East Main 1964 Limited | Extraterritorial | 2013 |
| Edward Johns Sanitary Limited | Manufacturing | 2020 |
| Elvin Property Lettings Ltd | Real estate | 2020 |
| Europe and Jersey Estates Ltd. | Real estate | 1996 |
| Europe Investments Limited | Real estate | 1995 |
| Fddj Ltd | Arts & recreation | 2025 |
| First Class Installations Limited | Construction | 2017 |
| Flyingaway Ltd | Wholesale & retail | 2024 |
| Game Vault Ayrshire Ltd | Information & communication | 2024 |
| Game Vault Scotland Ltd | Information & communication | 2025 |
| Gills Darvel Ltd | Wholesale & retail | 2018 |
| Glebedonston Limited | Wholesale & retail | 2012 |
| Glen 1971 Limited | Extraterritorial | 2013 |
| Health and Holistics Ltd | Public administration | 2017 |
| Hendryton First AID Training Ltd | Professional & scientific | 2022 |
| Hill Turf Development Limited | Construction | 2026 |
| Hooper Martin Estates Limited | Real estate | 2011 |
| Hyslop Mcbride Properties Limited | Real estate | 1946 |
| Independent Access Scotland Ltd | Manufacturing | 2024 |
| Invergower Estates Limited | Real estate | 2000 |
| IS459 Ceramics Ltd | Manufacturing | 2025 |
| Itsitworld Ltd | Information & communication | 2025 |
| JS Retail Ltd | Wholesale & retail | 2016 |
| Kades Kreation'Z Limited | Hospitality | 2025 |
| KC Janitorial Ltd | Administrative & support | 2020 |
| Kerr Property Services Ltd | Households | 2008 |
| Knight Engineering Hydraulics and Hoses Ltd | Professional & scientific | 2022 |
| Lanfine Brands Ltd | Manufacturing | 2019 |
| Lanfine Haulage Ltd | Transport & logistics | 2025 |
| Lanfine Limited | Manufacturing | 2019 |
| Lawtech Inc Ltd | Manufacturing | 2024 |
| Levrad Limited | Real estate | 2021 |
| Loudoun Veterinary Clinic Ltd | Professional & scientific | 2021 |
| LTK Site Engineering Ltd | Professional & scientific | 2026 |
| Luxury Lawn Care Ltd | Administrative & support | 2025 |
| MCM Taverns Ltd | Hospitality | 2016 |
| Medine Properties Limited | Real estate | 2018 |
| MI Casa SU Casa Property Ltd | Real estate | 2026 |
| MS GAS & Ashp Ltd | Construction | 2022 |
| Natural Harmony HT Ltd | Other services | 2021 |
| Opal Trust Limited | Other services | 2003 |
| Paul Hamilton Limited | Wholesale & retail | 2026 |
| Pinovate Ltd | Professional & scientific | 2024 |
| PJ Landscapes Ltd | Administrative & support | 2016 |
| Precision Design and Templating Limited | Professional & scientific | 2024 |
| PW FIC Limited | Other services | 2018 |
| R F Lindsay Limited | Hospitality | 2011 |
| R F Lindsay Properties Limited | Real estate | 2024 |
| Ramsay Rural Ltd | Professional & scientific | 2025 |
| Ranstone Estates Ltd | Real estate | 2021 |
| Real Spicy Ltd | Hospitality | 2026 |
| Red Tales Productions Ltd | Information & communication | 2022 |
| Repair IT ALL Limited | Construction | 2019 |
| Rhet Ayrshire & Arran Countryside Initiative | Education | 2004 |
| Saac (Kilmarnock) Limited | Financial services | 2019 |
| Scot Comm Services Ltd | Professional & scientific | 2018 |
| Scotland Services Limited | Administrative & support | 2017 |
| Skyecho Limited | Administrative & support | 2024 |
| Smartech Software Limited | Information & communication | 2022 |
| Storageit Limited | Administrative & support | 2014 |
| THE BEG Farms Limited | Agriculture & fishing | 2019 |
| THE Financial Claims Group Ltd | Financial services | 2018 |
| TOM Grant Plant Limited | Administrative & support | 2014 |
| Udul Retail Ltd | Wholesale & retail | 2016 |
| VIEW360 Scotland Limited | Professional & scientific | 2008 |
| Weststreetdarvrl Ltd | Wholesale & retail | 2025 |