| Addhere Technologies Limited | Information & communication | 2021 |
| Aeomica Ltd | Extraterritorial | 2009 |
| AI Sight Limited | Professional & scientific | 2022 |
| Alpha Subco Limited | Professional & scientific | 2020 |
| Applied Healthcare Limited | Administrative & support | 1984 |
| Ashton Governance Limited | Professional & scientific | 2020 |
| Ashton Risk Consulting Ltd | Administrative & support | 2018 |
| Avian Ecology Ltd | Professional & scientific | 2009 |
| Axentia Group Ltd | Professional & scientific | 2020 |
| Axentia Tech Limited | Information & communication | 2020 |
| Azinq Ltd | Information & communication | 2016 |
| B-B-L Protect Limited | Manufacturing | 2020 |
| Blocktrack Ltd | Information & communication | 2018 |
| Chatty Learning Ltd | Public administration | 2012 |
| Chester Diocesan Learning Trust | Education | 2025 |
| Congleton Garden Centre Ltd | Administrative & support | 2008 |
| Credit-Hero Ltd | Administrative & support | 2009 |
| Daresbury Equestrian Ltd | Agriculture & fishing | 2017 |
| DOW Schofield Watts Transaction Services (Leeds) LLP | | 2015 |
| DRM Supply Chain Consultancy Limited | Administrative & support | 2015 |
| DSW Angels (Carried Interest) LLP | | 2018 |
| EJ Peak Technology Solutions Limited | Information & communication | 2004 |
| Embankment Development Management Services Limited | Real estate | 2001 |
| Entire Property Direct Ltd | Real estate | 2024 |
| EXA Robotic Systems Ltd | Financial services | 2023 |
| Expervent Limited | Manufacturing | 2014 |
| Express Bidco Limited | Financial services | 2022 |
| Goddess International Limited | Other services | 2002 |
| Imago Consulting Limited | Professional & scientific | 2001 |
| Imio Software Solutions Limited | Information & communication | 2013 |
| Lumin Tech Limited | Information & communication | 2020 |
| Medcotec Ltd. | Professional & scientific | 2021 |
| Mercer and Sons Limited | Wholesale & retail | 1915 |
| Midnight Grovers Friends Ltd | Wholesale & retail | 2026 |
| Mishmash I O UK Ltd | Information & communication | 2019 |
| Moon and Back Videography Limited | Information & communication | 2017 |
| Myriad Contracts Limited | Construction | 2003 |
| Olsen Actuation UK Ltd | Professional & scientific | 2009 |
| Olsen Motion Dynamics Ltd | Professional & scientific | 2015 |
| Origami AI Ltd | Manufacturing | 2025 |
| PHD IH Growth LLP | | 2021 |
| PLASMA2X Limited | Manufacturing | 2023 |
| Quantum Science Ltd | Manufacturing | 2018 |
| RE Speciality Chemicals | | 1996 |
| RE Specialty Chemicals Limited | Manufacturing | 2017 |
| Resilient Space Innovations Ltd | Manufacturing | 2025 |
| ROBOTIZ3D Technology Ltd | Professional & scientific | 2024 |
| Signum Solutions Limited | Information & communication | 2003 |
| Sky Medical Technology Limited | Professional & scientific | 2006 |
| Sterling Trust Limited | Administrative & support | 1983 |
| Tatton Consulting Ltd | Professional & scientific | 2014 |
| Thinkittech Ltd | Information & communication | 2020 |
| Tynicare Ltd | Manufacturing | 2021 |
| Wadaro Solutions Limited | Information & communication | 2019 |
| Wattl Ltd | Information & communication | 2016 |
| Whitby Jennings Property Limited | Real estate | 2025 |
| Wilson Hastings Ltd | Professional & scientific | 2019 |
| WTC Tax Ltd | Other services | 2020 |