| A & W Plumbing & Heating Limited | Construction | 2008 |
| A K Wilson Roofing Ltd | Construction | 2026 |
| A P Thomson Ltd | Wholesale & retail | 2016 |
| A.E.G. Rigging and Antenna Services Ltd | Administrative & support | 2020 |
| AAE Vertex Limited | Hospitality | 2026 |
| Abbey Automation Limited | Professional & scientific | 2025 |
| Abbey Metal Ltd | Water & waste | 2014 |
| Access Solutions (SCO) Ltd | Construction | 2023 |
| ACM Engineering Services Limited | Construction | 2022 |
| Active Events Ltd | Arts & recreation | 2012 |
| Addiction Recovery Systems Limited | Administrative & support | 2018 |
| Adrian K Wilson Roofing Limited | Construction | 2009 |
| Aegir Fleet Solutions Ltd | Information & communication | 2026 |
| AJ Forestry Ltd | Agriculture & fishing | 2018 |
| AJ Traders Scot Limited | Wholesale & retail | 2021 |
| AJA Rail Ltd | Construction | 2018 |
| Alex Johnstone a I Services Limited | Agriculture & fishing | 2012 |
| All-Tec Builders Limited | Construction | 2015 |
| All-Tec Joiners Builders Limited | Construction | 2026 |
| Alltec Joiners & Builders Limited | Construction | 2023 |
| Altostratus Systems Ltd | Information & communication | 2014 |
| AM Building and Quality Services Ltd | Administrative & support | 2022 |
| American Groceries Ltd | Wholesale & retail | 2025 |
| Amjco Ltd | Real estate | 2023 |
| Anchor Lettings Limited | Real estate | 2023 |
| Anderson Engraving Ltd | Administrative & support | 2020 |
| Anderson RMS Limited | Professional & scientific | 2017 |
| Anderson's Jewellery Workshop Ltd | Wholesale & retail | 2019 |
| Andersons Valuation and Appraisal Limited | Financial services | 2025 |
| Andy Mason Access Ltd | Construction | 2014 |
| Angie Campbell Hair & Beauty Limited | Other services | 2012 |
| Angie Campbell Limited | Other services | 2026 |
| Animal Behaviour Clinic Ltd | Other services | 2023 |
| Antalya Traditional Turkish Barbers Ltd | Other services | 2025 |
| Arcmac Ltd | Hospitality | 2015 |
| Arete Contracts Ltd | Construction | 2017 |
| ARL Wilson Electrical Services Ltd | Construction | 2019 |
| Arran Protective Coatings Ltd | Administrative & support | 2025 |
| ASL Adams Limited | Wholesale & retail | 2017 |
| Atikan Ltd | Agriculture & fishing | 2022 |
| Atmosperse Ltd. | Professional & scientific | 2004 |
| Auld Clock Cafe Limited | Hospitality | 2017 |
| Aura Health & Beauty Ayrshire Ltd | Public administration | 2025 |
| Auto Locksmith Doctor Limited | Wholesale & retail | 2022 |
| Ayrshire & Glasgow Bricklaying Services Ltd | Construction | 2019 |
| Ayrshire Body and Paint Ltd | Wholesale & retail | 2026 |
| Ayrshire Holiday LET Cleaning Ltd | Administrative & support | 2023 |
| Bankside Stud Farm Limited | Agriculture & fishing | 2023 |
| Bano Foods Limited | Wholesale & retail | 2025 |
| Barra Holiday Lets Limited | Real estate | 2023 |
| Beithcraft Double Glazing Limited | Construction | 2018 |
| Bellvue Associates Ltd | Information & communication | 2025 |
| BEN Wilson Rigging Ltd | Construction | 2016 |
| Best for Bathrooms Ayrshire Limited | Construction | 2020 |
| Betty’S Bunkers Ltd | Real estate | 2025 |
| Bike Revs Ltd | Wholesale & retail | 2020 |
| Binning Stores (Dalry) Ltd | Wholesale & retail | 2013 |
| Birkentop Transport Services Limited | Transport & logistics | 2018 |
| Blueacres Holdings Ltd | Professional & scientific | 2025 |
| Bmac Heating Plumbing GAS Ltd | Construction | 2025 |
| Bmac Property Solutions Ltd | Real estate | 2022 |
| BON888 Limited | Manufacturing | 2026 |
| Borestone Repairs Limited | Wholesale & retail | 2002 |
| Borked IT Ltd | Information & communication | 2018 |
| Bowser Mclean Limited | Arts & recreation | 2024 |
| Brighthaus Ltd | Administrative & support | 2023 |
| Broadstone Consulting Solutions Ltd | Professional & scientific | 2019 |
| Bruce Wealth Consultancy Ltd | Professional & scientific | 2013 |
| BS79 Ltd | Information & communication | 2024 |
| Busby Deli Limited | Hospitality | 2024 |
| C N Gilbert Ltd | Manufacturing | 2013 |
| C&J Weddings & Party Hire Ltd | Administrative & support | 2022 |
| Cafe Creme Glasgow Ltd | Hospitality | 2020 |
| Cairn Visuals Limited | Professional & scientific | 2024 |
| Calistri Hotels (Dalry) Limited | Hospitality | 2010 |
| Capital Business Park Limited | Construction | 2016 |
| Capital Steadings Limited | Real estate | 2021 |
| Capital Steel Group Limited | Professional & scientific | 2008 |
| Capital Steel Limited | Manufacturing | 2004 |
| Carrie Marshall Media Ltd | Professional & scientific | 2020 |
| Carsehead Property Renovations Ltd | Real estate | 2023 |
| Castlepark Livestock Ltd | Agriculture & fishing | 2025 |
| Cc-Arch Ltd | Professional & scientific | 2024 |
| Ccorpp Limited | Construction | 2018 |
| CE Technical Ltd | Manufacturing | 2023 |
| Chef DU Jour Limited | Hospitality | 2024 |
| Clarity (Scotland) Limited | Construction | 2024 |
| Clashwhannon Holiday Park Ltd | Hospitality | 2020 |
| Clashwhannon Restaurant Ltd | Administrative & support | 2018 |
| Class 1 Elevators Ltd | Administrative & support | 2018 |
| Classic Events & Entertainments Ltd | Hospitality | 2025 |
| Client Communications Ltd. | Information & communication | 2011 |
| Clyde Escalators Limited | Manufacturing | 2016 |
| CO2 Compliance Limited | Water & waste | 2007 |
| Coffee JAM Limited | Hospitality | 2021 |
| Comicrazy Cafe Ltd | Wholesale & retail | 2017 |
| Comtek Enterprise Ltd | Wholesale & retail | 2022 |
| Crackin Sea Tours Limited | Transport & logistics | 2024 |
| CSW Joinery & Maintenance Ltd | Construction | 2023 |
| Ctmmechanicalservices Limited | Other services | 2022 |