| Indian Amber Ltd | Professional & scientific | 2024 |
| Inmuco Ltd | Education | 2026 |
| Innovation Technology Scotland Ltd | Professional & scientific | 2024 |
| Insured Solutions Ltd | Financial services | 2025 |
| Integrated Surgery Ltd | Health & social care | 2012 |
| Integri (International) Ltd | Professional & scientific | 2024 |
| Integri Developments Ltd | Construction | 2021 |
| Integri PM Ltd | Construction | 2023 |
| Intelligent Architecture Limited | Information & communication | 2018 |
| Intercultural Connections Initiative CIC | Professional & scientific | 2023 |
| Interflex Scotland Limited | Manufacturing | 1917 |
| Interpreting Academy Ltd | Other services | 2021 |
| Inverallan Advisory Limited | Professional & scientific | 2024 |
| Inveresk Mills Ltd | Real estate | 2026 |
| Ipwi Ltd | Information & communication | 2018 |
| Iris Inspirational Retreats Ltd | Hospitality | 2024 |
| Ironmills Dalkeith Ltd | Wholesale & retail | 2026 |
| Ironmills Developments Limited | Construction | 2007 |
| Ironmills Leasing Ltd | Transport & logistics | 2025 |
| Isaac Miller Limited | Wholesale & retail | 2022 |
| Itemp Limited | Information & communication | 2005 |
| J & I Scaffolding Contracts Ltd. | Construction | 2012 |
| J & I Scaffolding Equipment Ltd | Administrative & support | 2012 |
| J & J Cleaners and Beauty Limited | Administrative & support | 2025 |
| J DOW Limited | Wholesale & retail | 2017 |
| J Houston Consultancy Ltd | Professional & scientific | 2008 |
| J L Consultancy Group Limited | Professional & scientific | 2019 |
| J Murdoch Gardening Ltd | Administrative & support | 2026 |
| J&M Gardening Services & Landscaping Limited | Construction | 2016 |
| J&T Supply Chain Solutions Ltd | Professional & scientific | 2024 |
| J&V Better Home Ltd. | Construction | 2015 |
| J. Jordan Steel Fabricators Ltd. | Administrative & support | 2009 |
| J.Goree Ltd | Administrative & support | 2023 |
| J.J.C. Taxis Ltd. | Professional & scientific | 1998 |
| Jack Mortgages Ltd | Financial services | 2024 |
| Jack's Engineering Control Solutions Limited | Professional & scientific | 2018 |
| Jaksik Properties Ltd | Real estate | 2023 |
| James Paulin Transport Ltd | Transport & logistics | 2020 |
| James T Properties Ltd | Real estate | 2025 |
| Jamieson Joiners & Builders Limited | Construction | 2011 |
| JANA2017 Limited | Transport & logistics | 2019 |
| Jarek's Painting Ltd | Construction | 2022 |
| Jays Hair Ltd | Other services | 2024 |
| JB Food Industry Specialist Ltd | Administrative & support | 2017 |
| JD Naismith Surveying Ltd | Professional & scientific | 2025 |
| JD55 Sports Limited | Arts & recreation | 2025 |
| Jeff W Morren & Co. Ltd | Extraterritorial | 2013 |
| Jeheil and Co Ltd | Professional & scientific | 2021 |
| Jema Investments PLD Limited | Real estate | 2018 |
| JJ Foods Limited | Wholesale & retail | 2017 |
| Jofi Ltd | Professional & scientific | 2026 |
| John B. Little Ltd. | Construction | 2002 |
| John Bartholomew & SON Limited | Information & communication | 1999 |
| John Monteith Properties Limited | Real estate | 1948 |
| John Owen Photographer Limited | Professional & scientific | 2002 |
| Joinery Plumbing Elecrical Solutions Ltd | Construction | 2025 |
| Joli Cafe & Gifts Limited | Administrative & support | 2017 |
| Jonathan James O’Keefe Ltd | Hospitality | 2025 |
| JP DIT Ltd | Professional & scientific | 2019 |
| Jpmm Belmont Limited | Real estate | 2017 |
| Jppa Properties Limited | Other services | 2017 |
| Jrdn Group Limited | Financial services | 2025 |
| JRM Testing Services Limited | Information & communication | 2022 |
| JS Digital Ltd | Professional & scientific | 2020 |
| JS Elcano Limited | Real estate | 2025 |
| JS Legend Ltd | Professional & scientific | 2021 |
| JT Supplies (Edinburgh) Ltd | Wholesale & retail | 2025 |
| Jtfox Ltd | Health & social care | 2012 |
| Jupitr Ltd | Information & communication | 2025 |
| Jusi Ltd | Health & social care | 2019 |
| Just Perfect Landscaping Limited | Administrative & support | 2020 |
| Just Shot Ltd | Information & communication | 2023 |
| K C E & I Ltd | Construction | 2022 |
| K Dyet Ltd | Construction | 2026 |
| K. & I. Limited | Wholesale & retail | 1955 |
| KAI RAY Ltd | Real estate | 2024 |
| Kairo’S Kafe Ltd | Hospitality | 2025 |
| Kalyango Group Ltd | Real estate | 2019 |
| Kamo Holdings Ltd | Real estate | 2020 |
| Kamrai & Sons Ltd | Wholesale & retail | 2024 |
| Kanban Delivery Ltd. | Information & communication | 2016 |
| Kaswar Ltd | Wholesale & retail | 2025 |
| Katewell Quarry Limited | Real estate | 2006 |
| Katie Does Comms Ltd | Professional & scientific | 2023 |
| KAY Property Investments (Edinburgh) Ltd | Real estate | 2019 |
| Kayaas Ltd | Other services | 2017 |
| KB Harvesting Ltd. | Agriculture & fishing | 2012 |
| KEE Properties (Scotland) Ltd | Real estate | 2017 |
| Keith Goodwin Painting and Decorating Services Limited | Construction | 2017 |
| Keith Roseburgh Financial Ltd | Financial services | 2019 |
| Kempton Developments Ltd. | Real estate | 2006 |
| Kennisis Consulting Ltd | Health & social care | 2019 |
| Kennth Arthur Thomson Ltd | Extraterritorial | 2024 |
| Kenscot Property Ltd | Real estate | 2024 |
| Kerax Land Ltd | Administrative & support | 2022 |
| Key Contacts Letting Solutions Limited | Real estate | 2011 |
| Keystone Trading Ltd | Wholesale & retail | 2025 |
| Kgdmarine Ltd. | Professional & scientific | 2018 |
| KGM Joinery Ltd | Construction | 2025 |
| KH Fire Protection Ltd | Public administration | 2022 |