| Thomas Deco Limited | Construction | 2016 |
| Thomas Melrose Ltd | Water & waste | 2018 |
| Thrive Property Holdings (NO. 1) Limited | Real estate | 2026 |
| Ticb Ltd | Hospitality | 2006 |
| Timbex Creations Ltd | Manufacturing | 2018 |
| Tocher Research Ltd | Professional & scientific | 2025 |
| Todd Electrical Ltd | Construction | 2017 |
| TOG Property (Dalkeith) Limited | Construction | 2016 |
| Togamawa Business Consultants Ltd | Professional & scientific | 2019 |
| TOM Bell Joinery Limited | Manufacturing | 2002 |
| Tomas Tiling Ltd | Construction | 2019 |
| TON Platinum Property Ltd | Real estate | 2022 |
| Top G Barbers Ltd | Other services | 2023 |
| Topbranduk Ltd | Wholesale & retail | 2025 |
| Trade Boss Coaching Limited | Education | 2024 |
| Tradeboss-Ai Limited | Information & communication | 2025 |
| Tradeline Plumbing & Joinery Ltd | Construction | 2025 |
| Tradeteam Contracts Ltd | Construction | 2018 |
| Traquair Property Ltd | Real estate | 2020 |
| Triaene Solutions Ltd | Professional & scientific | 2024 |
| Troy Cafeteria Ltd | Hospitality | 2025 |
| Trunking Mate Ltd | Manufacturing | 2021 |
| TRZSDN7 Limited | Transport & logistics | 2026 |
| TTP Building Services Limited | Construction | 2008 |
| Turnbull & Pryde Services Limited | Construction | 2023 |
| Turnbull & Sons Joinery and Landscaping Ltd | Other services | 2025 |
| Turret Inns Limited | Real estate | 1999 |
| TW Scaffolding Contracts Limited | Construction | 2018 |
| U & a Scotland Ltd | Wholesale & retail | 2015 |
| UBK Training and Consulting Ltd | Professional & scientific | 2017 |
| Ugly BAT Creative Studio Ltd | Information & communication | 2024 |
| UH Superstore Ltd | Hospitality | 2023 |
| UK Structural Inspections Ltd | Professional & scientific | 2019 |
| Unilith Limited | Manufacturing | 1988 |
| Universal Interior Solutions Limited | Construction | 2023 |
| Untamed Collective Limited | Other services | 2025 |
| UP Here Media Ltd | Information & communication | 2019 |
| Upoxy Limited | Manufacturing | 2022 |
| Urban Dream Limited | Construction | 2015 |
| Utropolis Ltd | Information & communication | 2023 |
| VA Brothers Ltd | Hospitality | 2024 |
| Valentino's Fast Food (Fife) Ltd | Hospitality | 2019 |
| VC Platform Ltd | Wholesale & retail | 2020 |
| Vector Podiatry Ltd | Health & social care | 2026 |
| Velman Limited | Wholesale & retail | 2026 |
| Velpula's Ltd | Wholesale & retail | 2025 |
| Vencedor Limited | Wholesale & retail | 2025 |
| Vertical Access and Rigging Ltd | Construction | 2024 |
| Vertical Rigging Services Ltd | Professional & scientific | 2012 |
| VIA Barracuda Ltd | Hospitality | 2025 |
| Viadomo Ltd | Information & communication | 2025 |
| VIP Records Limited | Information & communication | 2005 |
| Vipondcontractors Ltd | Manufacturing | 2025 |
| Vismaya Limited | Information & communication | 2009 |
| Visser & Co Ltd. | Real estate | 2019 |
| Visser Commercial Property Investments Ltd | Real estate | 2023 |
| Visser Developments Ltd | Real estate | 2024 |
| Visser North East Property Investments Ltd | Real estate | 2025 |
| Visser Property Group Ltd | Financial services | 2026 |
| Visser Property Investments Ltd | Real estate | 2020 |
| Vital Space Coaching Ltd | Administrative & support | 2022 |
| Volcano Sports and Entertainment Ltd | Professional & scientific | 2022 |
| Volunteer Centre Midlothian | Health & social care | 2003 |
| Vonnehot Ltd | Wholesale & retail | 2025 |
| Vulcan Services Ltd | Construction | 2009 |
| Warehouse Leisure Group Ltd | Other services | 2018 |
| Waste Cloud Limited | Water & waste | 2023 |
| Waterston Landscapes Ltd | Administrative & support | 2019 |
| Waterworks Detailing Auto Ltd | Administrative & support | 2026 |
| Waybridge Developments Ltd. | Real estate | 2005 |
| Wealth Synergy Assets Ltd | Real estate | 2025 |
| Web-Art-Soft Ltd | Information & communication | 2017 |
| Webstar Games Ltd | Wholesale & retail | 2019 |
| Westerhouse Developments (Penicuik) Limited | Construction | 2005 |
| White & Co Property Ltd | Real estate | 2025 |
| Whitson Bathroom Co Ltd | Construction | 2024 |
| Wild Boar Bathrooms Ltd | Wholesale & retail | 2018 |
| Wilkinson Property Ventures Ltd | Real estate | 2023 |
| William Robertson & Sons Limited | Manufacturing | 1986 |
| WIN Life Competitions Ltd | Wholesale & retail | 2025 |
| Wind Innovations Ltd | Construction | 2018 |
| Wisdom Lighthouse Limited | Extraterritorial | 2021 |
| Wiser Developments Ltd | Construction | 2024 |
| Wiser Plumbing and Heating Ltd | Construction | 2022 |
| WJB Consultancy Services Ltd | Professional & scientific | 2015 |
| WMP Trading Ltd | Wholesale & retail | 2024 |
| Women's AID East and Midlothian Limited | Hospitality | 2008 |
| Woodburn Licensed Grocers(Dalkeith) Ltd | Wholesale & retail | 2020 |
| Woodburn Spice Limited | Hospitality | 2025 |
| Woodburn Store Ltd | Wholesale & retail | 2022 |
| Wopa Group Ltd | Hospitality | 2025 |
| Wraysbury Developments Ltd. | Real estate | 2003 |
| Wright Property Sourcing Limited | Real estate | 2025 |
| WSJ Services Ltd | Administrative & support | 2022 |
| XL Consultants Limited | Real estate | 2019 |
| XYZ Maps Ltd | Information & communication | 2013 |
| YAI Construction Management Services Limited | Construction | 2023 |
| YES Chef Consultancy Ltd | Professional & scientific | 2024 |
| Yilmaz Denli Ltd | Hospitality | 2024 |
| YOU Understood Ltd | Information & communication | 2020 |