| 3CF Ltd | Professional & scientific | 2025 |
| A & J Transfers Ltd | Transport & logistics | 2024 |
| Aberdour Festival Limited | Arts & recreation | 2016 |
| Advanced Dyno Solutions Ltd | Manufacturing | 2022 |
| Aero Specialists Limited | Professional & scientific | 2022 |
| Ajrp Ltd | Construction | 2015 |
| Apex Networx Ltd | Administrative & support | 2014 |
| Bay Financial Planning Ltd | Financial services | 2023 |
| Bayleaf DRB Ltd | Hospitality | 2024 |
| Beluga Lagoon Ltd | Information & communication | 2020 |
| Beyond Your Thinking Ltd | Other services | 2016 |
| Black Thistle FIC Limited | Financial services | 2026 |
| Buzzco Ltd | Other services | 2023 |
| Cashmaster (Holdings) Limited | Manufacturing | 2013 |
| Cashmaster International Limited | Manufacturing | 1992 |
| CE Structural Ltd | Professional & scientific | 2021 |
| Christies Accountants and Tax Advisors Ltd | Professional & scientific | 2017 |
| Clovecrest Limited | Construction | 2004 |
| Coast Care and Support Ltd | Health & social care | 2022 |
| Cross the T Limited | Professional & scientific | 2021 |
| Cullaloe Consulting Ltd | Professional & scientific | 2019 |
| D & H Design Ltd. | Professional & scientific | 2008 |
| Dalgety Bay Powder Coating Ltd | Manufacturing | 2021 |
| Dunallan Homes Ltd. | Construction | 1997 |
| Eastway Construction Limited | Construction | 1994 |
| Eastwayfife Ltd | Households | 2024 |
| Ecowin Ltd | Construction | 2012 |
| ESS Investments Scotland Limited | Real estate | 2024 |
| Findt Ltd | Mining & quarrying | 2026 |
| First Scottish Document Management Limited | Administrative & support | 2002 |
| Forth Grass Machinery Limited | Other services | 2012 |
| Forth Scientific Ltd | Professional & scientific | 2022 |
| Fruan Limited | Professional & scientific | 2017 |
| Glenburn Water Services Limited | Water & waste | 2017 |
| Graham Michael Publishing Ltd | Extraterritorial | 2025 |
| Grattan and Hynds (Holdings) Limited | Financial services | 2013 |
| Grattan and Hynds Contracts Limited | Construction | 2015 |
| Grattan and Hynds Group Limited | Construction | 2013 |
| Grattan and Hynds Limited | Construction | 1989 |
| GSM Electrical Fife Ltd | Construction | 2016 |
| H W Manufacturing Limited | Manufacturing | 2004 |
| Habilis Prime Ltd | Professional & scientific | 2022 |
| Halyard Developments Ltd | Real estate | 2019 |
| Hannah Whisky Merchant's Ltd | Wholesale & retail | 2012 |
| Harley's Bookkeeping & Tax Returns Ltd | Professional & scientific | 2019 |
| HM Enviro Ltd | Professional & scientific | 2022 |
| HY Spec Storage Ltd | Construction | 2021 |
| Ignis Manufacturing Services Ltd | Manufacturing | 2014 |
| Jamieson Decorators Ltd. | Construction | 2002 |
| JBM Glass & Glazing Ltd | Construction | 2019 |
| Just Golf 18 Ltd | Wholesale & retail | 2019 |
| KDM Enterprises Limited | Financial services | 2020 |
| L Dougall Cleaning Services Ltd | Administrative & support | 2024 |
| L'Art DU VIN Limited | Hospitality | 2007 |
| L.J.S. Properties Ltd. | Real estate | 1995 |
| LE Saux Consulting Ltd | Public administration | 2018 |
| Ledger Control Limited | Administrative & support | 1986 |
| Life Sciences Awards (Scotland) Limited | Hospitality | 2024 |
| LMS Construction Services Limited | Construction | 2022 |
| Lord Veterinary Services Ltd | Professional & scientific | 2014 |
| Luscar Holdings Ltd | Real estate | 2021 |
| Maccraig Properties Limited | Real estate | 2023 |
| Magic Mike Electrical Ltd | Construction | 2020 |
| Marrlie Limited | Wholesale & retail | 2025 |
| MB Subsea Ltd | Professional & scientific | 2022 |
| Merlin House Limited | Real estate | 2001 |
| MSP Transport Ltd. | Transport & logistics | 2007 |
| North Facades Limited | Construction | 2005 |
| Old School Enterprises Limited | Information & communication | 2005 |
| Osprey Properties Limited | Real estate | 2003 |
| Paint Inspection and Training Ltd | Construction | 2021 |
| Periculum Operational Risk Ltd | Professional & scientific | 2024 |
| Pinnacle Fitness Solutions Ltd | Manufacturing | 2019 |
| Powerphotonic Limited | Manufacturing | 2004 |
| Project Bowmore Bidco Limited | Financial services | 2021 |
| Project Bowmore Topco Limited | Financial services | 2021 |
| Protool Engineering Supplies Ltd | Wholesale & retail | 2018 |
| RA Electrical Inspection Ltd | Administrative & support | 2017 |
| Rbav Ltd | Other services | 2024 |
| RC Suite Ltd | Information & communication | 2020 |
| Reddley Properties Limited | Real estate | 2019 |
| Robertson Dance Academy Ltd | Education | 2020 |
| Rustic Eco Properties Ltd. | Construction | 2008 |
| SBP (Scotland) Limited | Professional & scientific | 2008 |
| SHB (Commercial) Limited | Arts & recreation | 2005 |
| SHB (Golf) Limited | Arts & recreation | 2005 |
| SHB Developments Limited | Real estate | 2002 |
| Sips (Holdings) Limited | Financial services | 2016 |
| Sips Homes Ltd | Construction | 2019 |
| Sips Homes Scotland Ltd | Construction | 2019 |
| Skin Unique Limited | Other services | 2019 |
| Slaywithang Limited | Extraterritorial | 2024 |
| Smarthealth Technologies Ltd | Information & communication | 2025 |
| Stephen Marshall Ltd | Transport & logistics | 2026 |
| THE Edge Agency Ltd | Professional & scientific | 2018 |
| THE Mortgage Fixer Ltd | Financial services | 2019 |
| THE Period House Development Company Limited | Real estate | 1997 |
| Tilly Hire Ltd | Administrative & support | 2024 |
| Todd Fish Tech Ltd | Manufacturing | 2014 |
| Transgressive North | Arts & recreation | 2011 |