| 21 SIX Holdings Limited | Professional & scientific | 2014 |
| 21 SIX Investments Limited | Financial services | 2020 |
| 21 SIX Limited | Information & communication | 2013 |
| 21 SIX Media Limited | Information & communication | 2018 |
| 21 SIX Platform Limited | Financial services | 2021 |
| 21SIX Publishing Limited | Information & communication | 2009 |
| Acorns (Southern) Propco Limited | Real estate | 2024 |
| American Sports Ltd | Information & communication | 2018 |
| ARJ Technical Services Ltd | Professional & scientific | 2008 |
| Bespoke GAS Ltd | Construction | 2015 |
| Bison Industrial Limited | Wholesale & retail | 2022 |
| Blizzard Media Ltd | Information & communication | 2011 |
| Ebitt Consulting Limited | Professional & scientific | 2017 |
| Exterior Clocks Ltd | Manufacturing | 2011 |
| Folding Pocket Ltd | Information & communication | 2020 |
| Format Social Limited | Information & communication | 2020 |
| Forward Press Ltd | Financial services | 2020 |
| Foziefabrications Ltd | Professional & scientific | 2024 |
| Fruit Studios Limited | Professional & scientific | 2006 |
| GA Commercial Solutions Limited | Administrative & support | 2007 |
| Hampshire and Isle of Wight Wildlife Trust | Other services | 1960 |
| Hewitt Britton Limited | Administrative & support | 2015 |
| Hudson Homes (Southern) Limited | Construction | 2019 |
| Infinity Od-L Ltd | Professional & scientific | 2020 |
| Inside Fighting Limited | Information & communication | 2020 |
| Jellyfish Solutions Limited | Manufacturing | 2006 |
| Jumbo Events Limited | Financial services | 2021 |
| Kitnocks Farms Limited | Agriculture & fishing | 1987 |
| Mcdowell Property Holdings Ltd | Financial services | 2026 |
| MJC Tree Surgeons Limited | Agriculture & fishing | 2003 |
| Movedia Limited | Information & communication | 2018 |
| Racecheck Limited | Information & communication | 2016 |
| Southcott Homes Limited | Construction | 2013 |
| Stag Homes (Southern) Ltd | Construction | 2019 |
| Tech Connect Group Limited | Administrative & support | 2019 |
| THE Good the BAD and the Rugby Ltd | Information & communication | 2020 |
| THE White House (Curdridge) Limited | Health & social care | 2003 |
| Trinorth Limited | Information & communication | 2002 |
| Volando Partners Limited | Financial services | 2021 |