| Rankeilour Farms Limited | Agriculture & fishing | 2015 |
| Rankein Corporate Ltd | Professional & scientific | 2020 |
| Rankein Group Ltd | Professional & scientific | 2020 |
| RAS Technology Ltd | Agriculture & fishing | 2023 |
| Rathillet Farms Limited | Agriculture & fishing | 2011 |
| RC76 Ventures Ltd | Real estate | 2025 |
| RDH Joinery Ltd | Construction | 2026 |
| Rebearth Ltd | Construction | 2015 |
| Red Lichtie Holdings Ltd | Financial services | 2025 |
| Red Star Property Rentals LLP | | 2015 |
| Reed Insulation Limited Liability Partnership | | 2007 |
| Reeryte Limited | Information & communication | 2026 |
| Renew Energy Services Ltd | Construction | 2025 |
| Rennick Development Limited | Construction | 2016 |
| Rennick Investments Limited | Administrative & support | 2019 |
| Rennick Property Limited | Construction | 2012 |
| Rennie Capital Limited | Financial services | 2024 |
| Rennie Group Limited | Administrative & support | 2008 |
| Resolute Fitness Limited | Arts & recreation | 2024 |
| Rethinking Change Ltd | Professional & scientific | 2020 |
| RG & SA Brown Ltd | Agriculture & fishing | 2025 |
| RGP Limited | Professional & scientific | 2010 |
| RHR Bathrooms & Kitchens Limited | Construction | 2024 |
| Richard Johnson (Drilling Fluids) Ltd. | Professional & scientific | 1997 |
| Ricks Plastering Ltd | Construction | 2022 |
| Ritchie QA Ltd | Professional & scientific | 2021 |
| RK Caravan Repairs Ltd | Other services | 2014 |
| RM Brown Ltd | Information & communication | 2023 |
| RM Motor Repairs Ltd | Wholesale & retail | 2023 |
| Rmha Limited | Hospitality | 2016 |
| Rmlmd & Partners LLP | | 2025 |
| Robb Developments (Scotland) Ltd | Construction | 2017 |
| Robb Partners LLP | | 2026 |
| Robb Properties (Scotland) Ltd. | Real estate | 2019 |
| Robb Real Estate Ltd | Real estate | 2023 |
| Robert Mason Contracts Limited | Construction | 1988 |
| Robert Mason Developments Limited | Construction | 2018 |
| Robertson & Smart (Builders) Limited | Construction | 1976 |
| Robertson Mcgregor Property Solutions Limited | Construction | 2011 |
| Robyn a Graham Ltd | Real estate | 2012 |
| Rochie Developments Limited | Real estate | 2008 |
| Rock and Spindle Ltd | Health & social care | 2022 |
| ROE & Deer Property Limited | Real estate | 2026 |
| Rofsie Limited | Professional & scientific | 2014 |
| Rohan Perth Ltd | Other services | 2022 |
| Rokin Consult Limited | Professional & scientific | 2023 |
| Rollos Law LLP | | 2012 |
| Rollos Town & Gown Limited | Real estate | 2003 |
| Rollos Trustee Services Limited | Professional & scientific | 2012 |
| Ronaldson Canines Limited | Other services | 2022 |
| Roolco Ltd | Real estate | 2015 |
| Rosas Ranch Ltd | Hospitality | 2026 |
| Roseangle Early Years Ltd | Education | 2025 |
| Ross Automation Ltd | Professional & scientific | 2016 |
| Ross Hood Ltd | Professional & scientific | 2018 |
| Rowan Private Investments Ltd | Financial services | 2015 |
| Roxburgh Capital Markets Ltd | Real estate | 2006 |
| RP Structural Design Ltd | Professional & scientific | 2025 |
| RR Whisky Ltd | Wholesale & retail | 2018 |
| RS Property Services(Maintenance) Limited | Administrative & support | 2011 |
| RUG Washers Limited | Other services | 2023 |
| Ruman Homes and Renovations Ltd | Construction | 2021 |
| Ruman Properties Limited | Construction | 2024 |
| Russell J Graham Ltd | Real estate | 2014 |
| Russell Mainstream Supply Limited | Wholesale & retail | 1994 |
| S Laing Electrical Limited | Manufacturing | 2020 |
| S M Graham Ltd | Agriculture & fishing | 2012 |
| S Mcdill Welding Limited | Manufacturing | 2015 |
| S. Saunas Limited | Other services | 2024 |
| S.C.R.U.M. Signature Ltd | Manufacturing | 2026 |
| Saffron's Cupar Ltd | Hospitality | 2024 |
| Sairfeet Partners | | 1990 |
| Salt and Pine Limited | Hospitality | 2018 |
| Saltire Associates Limited | Professional & scientific | 2017 |
| Saltire Corporation Limited | Information & communication | 2018 |
| Saltire Rare Malt Limited | Wholesale & retail | 2022 |
| Saltire Security Products Limited | Wholesale & retail | 1999 |
| Sandy Subsea Engineering Ltd | Mining & quarrying | 2010 |
| Sargeant Orthopaedics Ltd | Health & social care | 2025 |
| Scaramanga Trading Ltd | Wholesale & retail | 2010 |
| SCL Takeaway Ltd | Hospitality | 2023 |
| Sco-Tech (Services) Limited | Manufacturing | 2016 |
| Scooply Ltd | Manufacturing | 2022 |
| Scotch Whisky International Real Estate Limited | Financial services | 2022 |
| Scotch Whisky International Warehousing Limited | Administrative & support | 2022 |
| Scotch Whisky Investments Ltd | Manufacturing | 2021 |
| Scotrom 224 Ltd | Hospitality | 2024 |
| Scottish Ecological Design Association | Information & communication | 2011 |
| Sdde Ltd | Manufacturing | 2021 |
| Sea Spider Limited | Information & communication | 2018 |
| Seeca Ltd | Professional & scientific | 2026 |
| Senza RIT Limited | Real estate | 2020 |
| Serenity Wellbox Ltd | Wholesale & retail | 2025 |
| SES Staging Ltd | Construction | 2019 |
| Setter Properties Limited | Real estate | 2021 |
| Sgmsc Limited | Manufacturing | 2024 |
| Sgrl Holding Limited | Other services | 2013 |
| SH Phones Ltd | Wholesale & retail | 2026 |
| Shane Charlton Ltd | Extraterritorial | 2010 |
| Share the Chores Ltd | Arts & recreation | 2023 |