| 24-7 Property Maintenance Team Limited | Construction | 2016 |
| 2810 Consulting Ltd | Information & communication | 2016 |
| 4FRONT Farming Co Ltd | Agriculture & fishing | 2016 |
| 4S Talking Torque Limited | Professional & scientific | 2004 |
| 77 Cycles Limited | Manufacturing | 2026 |
| 88 Desserts Ltd | Wholesale & retail | 2026 |
| A & a Donaldson Ltd | Wholesale & retail | 2022 |
| A G Akermo & Sons Ltd | Construction | 2014 |
| A Mcpherson & Co Ltd | Manufacturing | 2015 |
| A.M. Tree Survey Specialist Ltd | Professional & scientific | 2023 |
| Aaron Andrews Ltd | Arts & recreation | 2018 |
| AB Appliance Care Limited | Manufacturing | 2010 |
| Aberdour Developments Limited | Construction | 2017 |
| Aberhill Metals Limited | Water & waste | 1986 |
| Access Archaeological Condition Services Ltd | Wholesale & retail | 2013 |
| Access Business Consultants Ltd. | Information & communication | 2012 |
| Ace Ladybank Ltd | Hospitality | 2021 |
| Ace Radio Taxis Ltd | Transport & logistics | 2020 |
| Achates Offshore Limited | Transport & logistics | 2019 |
| Active Propertyscape Ltd | Real estate | 2025 |
| Adam Barber Cupar Ltd | Other services | 2026 |
| Adam Riedi Arboriculture Ltd. | Professional & scientific | 2011 |
| Adamson Doors Limited | Wholesale & retail | 2012 |
| Adamson Group Limited | Real estate | 2019 |
| Adamson Holdings (Scotland) Limited | Financial services | 2024 |
| Adriatic Oriental Foods (UK) Ltd | Hospitality | 2024 |
| Aeronics Ltd | Manufacturing | 2024 |
| AES (Scotland) Limited | Manufacturing | 2003 |
| AES Fabrication CE Limited | Professional & scientific | 2018 |
| Agbags Packaging Limited | Wholesale & retail | 2022 |
| Agri-Bio Limited | Wholesale & retail | 1989 |
| AHL Contracts Ltd | Construction | 2026 |
| AIM Joinery Services Ltd | Construction | 2019 |
| Ainscough Contracting Limited | Hospitality | 2019 |
| AJD Medical Ltd | Health & social care | 2024 |
| Akku Dodd Hill Limited | Construction | 2023 |
| Akku Energy Limited | Energy | 2022 |
| Alan Bennie Motor Engineers Limited | Wholesale & retail | 2003 |
| ALB4 Bespoke Ltd | Construction | 2021 |
| Albahall Leasing Services Limited | Administrative & support | 2018 |
| Albany Property (Fife) Limited | Real estate | 2015 |
| Alder-Mckay Ltd | Administrative & support | 2025 |
| Alex Moffat Business Services Ltd | Construction | 2026 |
| Alexander Benjamin Associates Limited | Other services | 2019 |
| Alimond Limited | Administrative & support | 2012 |
| Alistair Thorpe Cupar Limited | Construction | 2011 |
| Allan Wyllie Consulting Limited | Administrative & support | 2020 |
| Allandale Limited | Construction | 2021 |
| AM Fitness Ltd | Arts & recreation | 2013 |
| AM Sportstours Limited | Administrative & support | 2009 |
| Amax Plant Hire Limited | Administrative & support | 2018 |
| Amber ST Andrews Ltd | Hospitality | 2026 |
| AMG Golf Limited | Manufacturing | 2019 |
| Anco Distributors Limited | Wholesale & retail | 2008 |
| Anderson Veitch & Company Limited | Wholesale & retail | 2007 |
| Andrew Bruce Contracts Limited | Professional & scientific | 2023 |
| Andrew Clayson Limited | Professional & scientific | 2009 |
| Andrew Laing Joiners Ltd | Manufacturing | 2026 |
| Angus Sawmilling Services Limited | Manufacturing | 2018 |
| ARC Architects Ltd | Professional & scientific | 2014 |
| Ardilla Landscapes Limited | Administrative & support | 2026 |
| Areon Play Ltd | Construction | 2023 |
| ARL Business Services Limited | Professional & scientific | 2003 |
| Armagnac Properties Limited | Construction | 2012 |
| Ascent Advisory Services Limited | Professional & scientific | 2021 |
| Ascent Projects Limited | Professional & scientific | 2012 |
| Ashgard Investments Limited | Real estate | 2015 |
| Ashgard Limited | Real estate | 2014 |
| Ashnor Ltd | Wholesale & retail | 2016 |
| Ashnor Properties Ltd | Real estate | 2017 |
| Ashures Ltd | Professional & scientific | 2017 |
| ASR Incorporated Ltd | Real estate | 2012 |
| Assess Marketing Ltd | Professional & scientific | 2025 |
| Astute (Scotland) Business Consultancy Limited | Professional & scientific | 2019 |
| AT the Sign of the Pelican Ltd | Administrative & support | 2015 |
| Atomised Co-Operative Ltd | Information & communication | 2008 |
| Aurora Private Equity Limited | Financial services | 2003 |
| AW Mcpherson Plant Hire Limited | Administrative & support | 2007 |
| B2B Flooring Ltd | Construction | 2016 |
| B2B Holdings Ltd | Real estate | 2021 |
| Backhouse Nurseries (York) Limited | Administrative & support | 2019 |
| Backhouse Rossie Heritage & Education Centre Limited | Administrative & support | 2018 |
| Bake Scotland Ltd | Hospitality | 2023 |
| Balass Lettings Ltd | Hospitality | 2020 |
| Balass Small Holdings Ltd | Hospitality | 2020 |
| Balbirnie Estate Developments Limited | Construction | 2005 |
| Balgarvie Court Limited | Professional & scientific | 1990 |
| Balgay Farming Company Limited | Professional & scientific | 1985 |
| Ballantyne Wealth Management Ltd | Financial services | 2018 |
| Ballet Grand Prix C.I.C. | Arts & recreation | 2025 |
| Balletto Monson C.I.C. | Arts & recreation | 2024 |
| Ballinbreich Limited | Professional & scientific | 2021 |
| Ballindalloch Hospitality Ltd | Hospitality | 2025 |
| Ballindalloch Property Ltd | Real estate | 2025 |
| Balmalcolm DEN Ltd | Real estate | 2023 |
| Balmeadowside Ltd | Construction | 2017 |
| Balmuir Estates Nominees Limited | Extraterritorial | 2000 |
| Baltilly Holdings Limited | Construction | 2021 |
| Bandirran Farm Limited | Agriculture & fishing | 2008 |
| BCM Microelectronics Limited | Information & communication | 2014 |