| Salaar’S Tandoori Limited | Hospitality | 2026 |
| Saltire Confectionery Limited | Manufacturing | 2018 |
| SAN Pettigrew Ltd | Real estate | 2017 |
| Saunderson Engineering Ltd | Professional & scientific | 2024 |
| Scott Allan Services Ltd. | Transport & logistics | 2019 |
| Scott Wham Architect Ltd | Professional & scientific | 2014 |
| Seals Perfect Scotland Ltd. | Professional & scientific | 2014 |
| Security Door Repairs Ltd | Manufacturing | 2021 |
| Sevtec Electrical Limited | Construction | 2025 |
| Sharon. Stewart Cleaning Limited | Real estate | 2026 |
| Shroud Box Services Limited | Manufacturing | 2018 |
| Silverbirch Contracts Limited | Construction | 2011 |
| Simply Toppers Limited | Extraterritorial | 2020 |
| SJS SJS Ltd | Wholesale & retail | 2025 |
| SKC Property Holdings Ltd | Real estate | 2026 |
| SKC Renewable Services Ltd | Professional & scientific | 2024 |
| Smashedcumnock Limited | Hospitality | 2025 |
| Sorcerer Property Ltd | Real estate | 2025 |
| Spittal Haulage Scotland Limited | Transport & logistics | 2015 |
| SPY Car Wash Ltd | Other services | 2020 |
| Squarely Circle Solutions Ltd. | Administrative & support | 2024 |
| SR. Leon Ltd | Wholesale & retail | 2019 |
| SS Cleaning Services Ltd | Administrative & support | 2023 |
| Stark Adventures Ltd | Administrative & support | 2025 |
| Step TAE IT Ltd | Health & social care | 2025 |
| Stepend Lettings Ltd | Real estate | 2025 |
| Strategic Support Services Ltd | Professional & scientific | 2017 |
| Subfusion Ltd | Construction | 2023 |
| Sundrum Castle Residents Association Limited | Real estate | 2015 |
| Sunnyside Renewables Ltd. | Professional & scientific | 2012 |
| SWEETTREATS7867 Ltd | Hospitality | 2026 |
| T French & SON (Holdings) Ltd | Extraterritorial | 2012 |
| T French & SON Ltd | Transport & logistics | 2005 |
| T. French & SON (Earthwork) Limited | Administrative & support | 2000 |
| TAL Safety Solutions Ltd | Professional & scientific | 2016 |
| Tanner's Carpets Ltd | Wholesale & retail | 2025 |
| Tarar Grocers Ltd | Wholesale & retail | 2025 |
| TDT GMS Ltd | Health & social care | 2023 |
| Tesla Private Hire Cumnock Ltd | Transport & logistics | 2024 |
| TFM Construction Ltd | Construction | 2025 |
| THE 8789 Co Ltd | Manufacturing | 2018 |
| THE Big Mouth Distilling Co. Ltd | Extraterritorial | 2014 |
| THE Bride Bible Ltd | Administrative & support | 2025 |
| THE Calm Chameleon Ltd | Health & social care | 2020 |
| THE Coach House Inn Muirkirk Ltd | Hospitality | 2025 |
| THE Coffee POD Symington Ltd | Hospitality | 2025 |
| THE Dumfries Arms Hotel Ltd | Hospitality | 2018 |
| THE Fitness and Health Box Ltd | Arts & recreation | 2019 |
| THE Instant Card Company Ltd | Manufacturing | 2026 |
| THE King's Foundation | Arts & recreation | 2007 |
| THE Little Posh Company Ltd | Arts & recreation | 2024 |
| THE Lost Distillery Company Limited | Wholesale & retail | 2013 |
| THE Masala Cafe Ltd | Hospitality | 2026 |
| THE Revive Clinic C.I.C. | Arts & recreation | 2024 |
| THE Steamship SIR Walter Scott Limited | Transport & logistics | 2004 |
| THE Steamship SIR Walter Scott Trust | Arts & recreation | 2004 |
| THE Wage Shop Cumnock Ltd | Administrative & support | 2025 |
| THE Way Forward 2045 Limited | Professional & scientific | 2020 |
| THE Wretched Company Ltd | Information & communication | 2024 |
| Therapy Hand Ltd | Manufacturing | 2020 |
| Thistle Inn @Skerington Limited | Hospitality | 2025 |
| Thistle Transport (Scotland) Ltd. | Extraterritorial | 2008 |
| TI Capital Ltd | Real estate | 2019 |
| Timbermills Limited | Wholesale & retail | 2006 |
| Top Dog Clothes and Sports Goods Ltd | Wholesale & retail | 2024 |
| Tresani Ltd | Wholesale & retail | 2025 |
| Trustcare Group Limited | Professional & scientific | 2019 |
| Trustcare International Ltd. | Other services | 2007 |
| Trustcare Safety Solutions Ltd | Financial services | 2024 |
| Turtle Turtle Ltd | Other services | 2015 |
| Twelve Weeks Limited | Construction | 2020 |
| Urban Hair Academy Ltd | Other services | 2020 |
| V Direct Ltd | Wholesale & retail | 2013 |
| Vakn Collection Ltd | Financial services | 2026 |
| Valorant Heating Solutions Ltd | Construction | 2025 |
| VAT (UK) Limited | Professional & scientific | 2021 |
| VCP Commercial Limited | Professional & scientific | 2025 |
| VEHICLES4WORK Ltd | Administrative & support | 2017 |
| Vertree Limited | Manufacturing | 2015 |
| Vestigare Canine & Equine Ltd | Other services | 2019 |
| Veterans of Scottish Motorsport Association | Other services | 2006 |
| Visions (Cumnock) Limited | Arts & recreation | 2004 |
| VLR Rail Ltd | Administrative & support | 2024 |
| Volkstec (AYR) Ltd | Wholesale & retail | 2026 |
| W & J Smith Holdings Ltd | Financial services | 2021 |
| W&E Lammie Limited | Construction | 2019 |
| W&Z Convenience Ltd | Wholesale & retail | 2024 |
| W.J. Mclean Engineering Limited | Professional & scientific | 1995 |
| Wellsdone Ltd | Professional & scientific | 2024 |
| Windyparkservice UK Ltd | Manufacturing | 2019 |
| Woolly Willow Limited | Wholesale & retail | 2020 |
| WR (Scotland) Ltd | Professional & scientific | 2018 |
| WR Accountants Ltd. | Professional & scientific | 2018 |
| WRC VAW Limited | Professional & scientific | 2018 |
| WRC Zebs Ltd | Professional & scientific | 2018 |
| WRK Solutions Limited | Wholesale & retail | 2010 |
| YH Admin Services Limited | Administrative & support | 2024 |
| Young Brothers LLP | | 2013 |
| Zagreus Empire Ltd | Other services | 2025 |
| Zed's Superstore Ltd | Wholesale & retail | 2025 |