| 1ST Choice Home Improvements Scotland Ltd | Construction | 2022 |
| 23X.NET Ltd | Information & communication | 2010 |
| 286 Solutions Ltd. | Professional & scientific | 2015 |
| 2M Builders Limited | Construction | 2021 |
| 3C Coachbuilder Limited | Manufacturing | 2017 |
| 5 Star Turkish Barbers Ltd | Other services | 2019 |
| A&A Spittal Limited | Wholesale & retail | 2016 |
| A.T. Mclean Driveway, Patio & Groundwork Specialists Ltd | Construction | 2026 |
| AA ANP Locum Ltd | Health & social care | 2026 |
| AAA Landscapers Limited | Administrative & support | 2021 |
| ABH Joinery Limited | Construction | 2018 |
| Action Engineering Ltd | Manufacturing | 2025 |
| Adam Mcpheators Limited | Construction | 2013 |
| Aesthetic Touch Clinic Ltd | Other services | 2025 |
| Afton (Holdings) Ltd | Financial services | 2017 |
| Afton Builders Limited | Construction | 2023 |
| Air Review Ltd | Arts & recreation | 2025 |
| Air Smith Limited | Wholesale & retail | 2022 |
| AJ Green International Ltd | Wholesale & retail | 2025 |
| AJ Ranjha Ltd | Transport & logistics | 2023 |
| AKO News Ltd | Wholesale & retail | 2019 |
| Alcorn Contracts Ltd | Construction | 2023 |
| Alexander Muir Funeral Directors Limited | Other services | 2018 |
| Amcn Renewables Ltd | Professional & scientific | 2024 |
| ANA 5 Stars Ltd | Other services | 2026 |
| ANDREA15 Ltd | Wholesale & retail | 2025 |
| Angel Nails & Spa 29 Ltd | Other services | 2025 |
| Angel Tuning Scotland Ltd | Wholesale & retail | 2022 |
| Apex Roofing and Building Specialists Ltd | Construction | 2026 |
| Archie Kane Ltd | Arts & recreation | 2021 |
| Arkt Coachworks Ltd | Manufacturing | 2019 |
| Arowe Electrics Ltd | Manufacturing | 2018 |
| AS Sports Therapy Ltd | Other services | 2025 |
| ATK Collective Ltd | Financial services | 2026 |
| ATT Cumnock Ltd | Hospitality | 2025 |
| Auchinleck Community Development Initiative | Health & social care | 2005 |
| Aurora Interiors (Scotland) LLP | | 2021 |
| AYR Fight Team Limited | Arts & recreation | 2024 |
| Ayrshire Care Solutions Ltd | Health & social care | 2015 |
| Ayrshire Craft Distillers Ltd | Manufacturing | 2017 |
| Ayrshire FAB Ltd | Manufacturing | 2025 |
| Ayrshire Leasing Ltd. | Administrative & support | 2019 |
| Ayrshire Memorials Ltd | Information & communication | 2002 |
| Ayrshire Roughcasting Ltd | Construction | 2019 |
| Ayrshire Travel Ltd. | Transport & logistics | 2019 |
| B and G Fitness (Scotland) LLP | | 2021 |
| BA & EL Whyte Limited | Extraterritorial | 2007 |
| Baird Welding and Mechanical Ltd | Manufacturing | 2026 |
| Baljaffray Properties Limited | Real estate | 2000 |
| Balmoral Funeral Directors AYR Ltd | Other services | 2020 |
| Balmoral Irvine Ltd | Other services | 2021 |
| Barber Republic Ltd | Other services | 2021 |
| Barlosh Luxury Glamping Pods Ltd | Hospitality | 2021 |
| Barony Golf LAB Ltd | Education | 2026 |
| Barrhill Stores Limited | Wholesale & retail | 2019 |
| Barshare Stores Limited | Wholesale & retail | 2022 |
| BC Civils & Groundworks Ltd | Construction | 2021 |
| Beauty & Wellness Products Ltd | Other services | 2026 |
| Beauty by Irene Ltd | Other services | 2023 |
| Belston Plasma Profiles Ltd | Manufacturing | 2020 |
| Benn's Global Catering Ltd | Hospitality | 2024 |
| BGS Auto Repairs Ltd | Wholesale & retail | 2022 |
| Big Bear Investment Group Limited | Real estate | 2019 |
| BK Building & Repairs Ltd | Construction | 2025 |
| Blackstone Clydesdales Ltd | Agriculture & fishing | 2024 |
| Blackwood Farms Limited | Agriculture & fishing | 2023 |
| Blackwood Leisure (Knockroon) Ltd | Hospitality | 2025 |
| Blackwood Retail Ltd | Wholesale & retail | 2021 |
| Bloom Residential Care Limited | Health & social care | 2025 |
| BMG Surveys Limited | Construction | 2011 |
| Boath Properties Ltd | Real estate | 2012 |
| Bonnie Renovations Ltd | Real estate | 2021 |
| Boss Civils and Engineering Ltd | Construction | 2025 |
| Boss Kitchen (Scotland) Ltd | Hospitality | 2024 |
| Boss Rentals Ltd | Administrative & support | 2026 |
| Boswell Travel Ltd | Transport & logistics | 2023 |
| Bounce N Roar Ltd | Arts & recreation | 2025 |
| Brae Rose Consultancy Ltd | Professional & scientific | 2025 |
| Brilliantly Different Limited | Professional & scientific | 2017 |
| Britannia Sport and Exercise Science Academy Ltd | Professional & scientific | 2016 |
| Brix Joinery Limited | Manufacturing | 2022 |
| Bruce Lorimer Limited | Transport & logistics | 2021 |
| BSK Commercial Ltd | Construction | 2024 |
| Buchanan Surveyors Ltd | Professional & scientific | 2020 |
| Burnanne Spirits Ltd | Manufacturing | 2022 |
| Burnet (Fairfield Licence) Limited | Professional & scientific | 1993 |
| Burnock Mill Lodges Limited | Hospitality | 2023 |
| Burnsland Enterprise Group CIC | Other services | 2018 |
| C&K Cleaning Services Ltd | Administrative & support | 2017 |
| Cairns Builders (Scotland) Ltd. | Construction | 2011 |
| Caldwell Sheeting Limited | Construction | 2021 |
| Calton Renewables Limited | Energy | 2012 |
| Campbell Holdings (Scotland) Ltd | Financial services | 2018 |
| Campbell Homes (Afton Braes) Limited | Construction | 2017 |
| Campbell Homes (Sorn) Limited | Construction | 2021 |
| Campbell Homes (Tarbolton) Limited | Construction | 2024 |
| Campbell Homes Cumnock Limited | Construction | 2017 |
| Campbell Management Ltd | Financial services | 2026 |
| Capital Development Consultants LLP | | 2013 |
| Capital Estates (Scotland) Limited | Real estate | 2015 |