| Rhubarb Lime Ltd | Hospitality | 2013 |
| Rice Products Limited | Wholesale & retail | 2009 |
| RJR Groundworks Ltd | Construction | 2025 |
| RNR Electrical Ltd | Construction | 2022 |
| Ross Bell Associates Ltd | Financial services | 2014 |
| Rossal Holdings Limited | Financial services | 2024 |
| Rowan Integrated Management Systems Limited | Manufacturing | 2010 |
| RTS Forestry Limited | Financial services | 2017 |
| RTS Limited | Agriculture & fishing | 1984 |
| Ryanf Engineering Limited | Professional & scientific | 2019 |
| S Michael Hospitality Limited | Hospitality | 2023 |
| S&L Hospitality Ltd | Hospitality | 2018 |
| SAX Players Scotland Ltd | Arts & recreation | 2022 |
| Scoor Estate Limited | Agriculture & fishing | 1996 |
| Scotdance International Limited | Arts & recreation | 1986 |
| Scots Style Safaris Management Limited | Hospitality | 2011 |
| Scott Alexander Consulting Limited | Professional & scientific | 2022 |
| Secaura Solutions Limited | Wholesale & retail | 2017 |
| Securename Limited | Professional & scientific | 1990 |
| Security Solutions (Scotland) Ltd | Manufacturing | 2011 |
| Servelogic Ltd | Information & communication | 2026 |
| Shakoor Properties Ltd | Construction | 2021 |
| Short-Term Vacation Properties Ltd | Hospitality | 2023 |
| Sika Group Ltd | Construction | 2024 |
| Sirple Games CIC | Other services | 2026 |
| SJR Caravan Storage Ltd | Other services | 2020 |
| Snakatak (Crieff) Ltd | Hospitality | 2016 |
| SPACE21 Creative Marketing Ltd | Professional & scientific | 2022 |
| SPB CAP Ltd | Real estate | 2025 |
| Sports Academy (Scotland) Limited | Professional & scientific | 2010 |
| Srps Holdings Ltd | Wholesale & retail | 2023 |
| ST Fillans Community Trust Limited | Arts & recreation | 2007 |
| ST Linton Ltd | Hospitality | 2015 |
| Strathearn Ecology Limited | Professional & scientific | 2022 |
| Strathearn Fire Protection Ltd | Administrative & support | 2025 |
| Strathearn Hygiene Services Ltd | Administrative & support | 2024 |
| Strathearn Optical Limited | Wholesale & retail | 2019 |
| Strathearn Pest Control Ltd | Administrative & support | 2023 |
| Strathearn Print Ltd | Manufacturing | 2019 |
| Strathearn Taxi's Ltd | Transport & logistics | 2025 |
| Strathearn Tyres Limited | Wholesale & retail | 2004 |
| Strathearn Vets Limited | Professional & scientific | 2025 |
| Strathseal Ltd | Construction | 2026 |
| Stratton Financial Limited | Professional & scientific | 2024 |
| Strowan Consultants Ltd | Professional & scientific | 2018 |
| Strowan Joinery Limited | Construction | 2013 |
| Strowan Moto Trials Ltd | Wholesale & retail | 2019 |
| Stuart Cramb Joiners Ltd | Construction | 2026 |
| Stuart Roberts Joinery Limited | Construction | 2022 |
| Subsguru Ltd | Information & communication | 2025 |
| Sukha (Scotland) Ltd | Hospitality | 2021 |
| Sun and Moon Limited | Hospitality | 2018 |
| Sweeney Plumbing & Heating Ltd | Construction | 2017 |
| T & G Property Services Ltd | Hospitality | 2016 |
| Taylor Construction (Scotland) Ltd. | Construction | 2006 |
| Tayside Home Care Limited | Public administration | 2019 |
| Teachknology Ltd | Information & communication | 2023 |
| Teal Mango Limited | Professional & scientific | 2024 |
| Tectactoe Ltd | Information & communication | 2020 |
| TEE Towel Co. Limited | Wholesale & retail | 2025 |
| Telework Scotland Ltd. | Professional & scientific | 1999 |
| Terra Food and Drink Company Ltd | Manufacturing | 2023 |
| TH15 Limited | Professional & scientific | 2010 |
| THE Abbotsford@The Sirple Ltd | Hospitality | 2023 |
| THE Abercairny Estates Limited | Agriculture & fishing | 1924 |
| THE Bridge Awards Ltd | Administrative & support | 2013 |
| THE Cairn Compass CIC | Administrative & support | 2025 |
| THE Chinese Tea Club Limited | Health & social care | 2025 |
| THE Clydesdale Horse Society | Other services | 2000 |
| THE Comrie Pet People Ltd | Other services | 2026 |
| THE Crown@The Sirple Ltd | Hospitality | 2022 |
| THE Dunfermline Car Club Ltd. | Arts & recreation | 1963 |
| THE Four Seasons Hotel (Perthshire) Limited | Hospitality | 2017 |
| THE Glass Onion (Crieff) Limited | Wholesale & retail | 2020 |
| THE Great Outdoor Gym Company (Scotland) Limited | Arts & recreation | 2021 |
| THE Heartland Radio Foundation Limited | Arts & recreation | 1991 |
| THE Kelvin Consultants Limited | Professional & scientific | 1986 |
| THE Meadow@The Sirple Ltd | Hospitality | 2026 |
| THE Online Business 2025 Ltd | Wholesale & retail | 2025 |
| THE Proper Brand Limited | Wholesale & retail | 2015 |
| THE Schiehallion Property Company Limited | Construction | 2015 |
| THE Sirple Ltd | Hospitality | 2017 |
| THE Snak-Atak Catering Ltd | Hospitality | 2026 |
| THE Strathearn Gallery Limited | Wholesale & retail | 2013 |
| THE Sun Salon Limited | Other services | 2026 |
| THE Tree LAB (Perthshire) Ltd | Agriculture & fishing | 2024 |
| THE Vikki Bar Ltd | Hospitality | 2019 |
| THE West END Electrical Services Ltd | Professional & scientific | 2014 |
| THE Wright Joinery Ltd | Construction | 2025 |
| Thirldene Limited | Financial services | 2024 |
| Tomaknock Services Limited | Mining & quarrying | 2019 |
| Tomatin Trading Company Limited | Real estate | 2010 |
| Tomnah'A Market Garden Ltd | Agriculture & fishing | 2015 |
| Tpitb Limited | Hospitality | 2025 |
| TRADEB2B Limited | Information & communication | 2018 |
| Traynor Accounting Ltd | Professional & scientific | 2008 |
| Treaclesponge Ltd | Real estate | 2023 |
| Trex Ecology Ltd | Professional & scientific | 2021 |
| Trowan Limited | Construction | 2024 |
| Truckee Holdings Limited | Financial services | 2021 |