| DB Performance Coaching Limited | Education | 2020 |
| Decade 5 Ltd | Administrative & support | 2019 |
| Dedo Food Limited | Hospitality | 2025 |
| Defence Association of Primary Care Ltd | Administrative & support | 2024 |
| Demx Consulting Ltd | Construction | 2019 |
| Denhes Ltd | Professional & scientific | 2009 |
| Designing Signs Ltd | Manufacturing | 2023 |
| Digitise My Business UK Ltd | Extraterritorial | 2023 |
| Dimut Limited | Real estate | 2008 |
| Diverseot Ltd | Health & social care | 2024 |
| DM Security Solutions Limited | Professional & scientific | 2016 |
| DMS Consulting (UK) Limited | Professional & scientific | 2009 |
| Dolphin Technical Solutions Ltd | Information & communication | 2025 |
| Dont Poke the Bear Limited | Construction | 2012 |
| Douglas & Anthony Ltd | Manufacturing | 2019 |
| DOWN2ERF Limited | Information & communication | 2001 |
| Dragn Labs UK Ltd | Manufacturing | 2025 |
| DS1-SPORTS Ltd | Wholesale & retail | 2020 |
| Duck & Dog Limited | Wholesale & retail | 2013 |
| Dunton Bassett Agri Organics Ltd | Water & waste | 2026 |
| Dust Bros Ltd | Manufacturing | 2024 |
| Earthworm Media Ltd | Information & communication | 2023 |
| East & West Music Limited | Arts & recreation | 2018 |
| East European Construction Limited | Construction | 2012 |
| East European Developments Limited | Construction | 2025 |
| Eastoak Limited | Real estate | 1997 |
| Eaton Advisory and Consultancy Ltd | Information & communication | 2023 |
| EC People Consulting Ltd | Professional & scientific | 2022 |
| Ecohausiq Ltd | Information & communication | 2025 |
| EDD & I Limited | Wholesale & retail | 2025 |
| Education Select Ltd | Education | 2025 |
| Eefeez Ltd | Wholesale & retail | 2025 |
| Ehcp Writers Limited | Administrative & support | 2016 |
| Ehouse Interiors Ltd | Wholesale & retail | 2020 |
| Eight Bells Hawkhurst Limited | Hospitality | 2024 |
| Electronic Specifier Limited | Information & communication | 2008 |
| Electronics Datasheets Limited | Administrative & support | 2013 |
| Elephant in My Handbag Limited | Wholesale & retail | 2013 |
| Elite Property Solutions (SE) Limited | Real estate | 2021 |
| ELITE2024 Ltd | Other services | 2024 |
| Elizabeth Iris Limited | Administrative & support | 2020 |
| ELK Promotions Sussex Ltd | Administrative & support | 2019 |
| Ella Barwick Interior Design Ltd | Professional & scientific | 2024 |
| Elsmore Creative Marketing Limited | Professional & scientific | 2003 |
| Embers Woodcraft C.I.C. | Health & social care | 2024 |
| Emblehope Limited | Arts & recreation | 2026 |
| Emergency Sprill Productions Ltd | Professional & scientific | 2011 |
| Emperor Tours Limited | Administrative & support | 2004 |
| Employment Legal Matters Ltd | Professional & scientific | 2007 |
| Envirorend (S.E.) Ltd | Construction | 2013 |
| Envirorend S-Works Ltd | Construction | 2025 |
| Epicair Ltd | Wholesale & retail | 2005 |
| Equinate Ltd | Professional & scientific | 2026 |
| Eres Properties & Real Estate Ltd | Real estate | 2026 |
| Ermwell Limited | Construction | 2022 |
| Ernest Bowles & Sons Limited | Agriculture & fishing | 1950 |
| Eternal Build Limited | Construction | 2016 |
| Eternal Living Limited | Real estate | 2023 |
| Etool Global UK Ltd | Professional & scientific | 2015 |
| Eurohouse Limited | Real estate | 1983 |
| European Timber Solutions Limited | Professional & scientific | 2010 |
| Euthymia Services Limited | Administrative & support | 2026 |
| Evan's Food Ltd | Hospitality | 2007 |
| Evermark Studio Ltd | Wholesale & retail | 2025 |
| Everything Outside (Landscaping) Limited | Administrative & support | 2025 |
| Everything’S Mental! Ltd | Other services | 2024 |
| Executive Sports Limited | Arts & recreation | 1982 |
| Expedeck Ltd | Information & communication | 2022 |
| Fairford Limited | Wholesale & retail | 1996 |
| Falcon Lease Ltd | Administrative & support | 2019 |
| Falkland Forever Limited | Agriculture & fishing | 2020 |
| Family Care UK Limited | Health & social care | 2003 |
| Fancy Pants Emporium Ltd | Wholesale & retail | 2020 |
| Fang Tian Well-Being Ltd | Health & social care | 2021 |
| Farriers Courtyard Limited | Real estate | 2015 |
| FCG Surveyors Ltd | Construction | 2024 |
| FD Surveys Ltd | Extraterritorial | 2023 |
| Fernshire Limited | Construction | 1985 |
| Ferraro Properties Limited | Construction | 2000 |
| Fettes Real Estate Advisors Ltd | Real estate | 2015 |
| Fierce Content Ltd | Professional & scientific | 2023 |
| Fight Corruption in Africa | Administrative & support | 2010 |
| Figment Limited | Arts & recreation | 2003 |
| FIN DE Guerre Strategies Limited | Professional & scientific | 2018 |
| Finders International Limited | Manufacturing | 1990 |
| Fine Homes and Interiors Limited | Other services | 2007 |
| Fisika Ltd | Extraterritorial | 2025 |
| Five High Street Ltd | Real estate | 2024 |
| Flock and Fleece Ltd | Agriculture & fishing | 2019 |
| Football Club Hertz Limited | Arts & recreation | 2025 |
| Forbes Profit Collectables Ltd | Wholesale & retail | 2021 |
| Forbes Profit Ltd | Information & communication | 2020 |
| Forget HER NOT Ltd | Wholesale & retail | 2025 |
| Forrest Carers Limited | Public administration | 2025 |
| Forsham Oak Ltd | Construction | 2023 |
| Fosten Green Consulting Limited | Information & communication | 2017 |
| Foster Financials Ltd | Professional & scientific | 2025 |
| Foster Scaffolding Ltd | Construction | 2023 |
| Fountain House Freeholders Limited | Households | 2006 |
| FOX Cooper Property Ltd | Construction | 2021 |