| Steven Page Tours Limited | Arts & recreation | 2024 |
| Stomarty Ltd | Wholesale & retail | 2026 |
| Stuart LEE Transport Limited | Transport & logistics | 2025 |
| Subsea Inspection & Test Services Limited | Professional & scientific | 2007 |
| Suvam Retail Limited | Wholesale & retail | 2020 |
| Suvshree Catering Ltd | Hospitality | 2025 |
| Swift Scaffolding Scotland Ltd | Construction | 2023 |
| Sylva Care Partners Ltd | Health & social care | 2025 |
| Sync Point Limited | Wholesale & retail | 2025 |
| SÓ Property Group Limited | Real estate | 2024 |
| SÓ Supplies Group Limited | Construction | 2024 |
| T Power Transmission Ltd | Professional & scientific | 2025 |
| TAN4LESS Limited | Other services | 2015 |
| Tanxpress Dundee Limited | Other services | 2022 |
| Tanxpress Limited | Other services | 2017 |
| TAY Hardware Ltd | Wholesale & retail | 2023 |
| Technically Ginger Ltd | Professional & scientific | 2023 |
| Tfaqist Ltd | Wholesale & retail | 2026 |
| THE Better Life Blueprint Ltd | Information & communication | 2026 |
| THE Blazing Stone Ltd | Hospitality | 2025 |
| THE Caledonian Craft Beer Merchant Ltd | Wholesale & retail | 2018 |
| THE Fife Veranda Company Ltd | Construction | 2025 |
| THE Forecourt Group Ltd | Wholesale & retail | 2019 |
| THE Hair Spa Scotland Ltd | Other services | 2024 |
| THE Moray Kelty Ltd | Arts & recreation | 2025 |
| TVN Properties Ltd | Real estate | 2025 |
| U Save Grocers Ltd | Extraterritorial | 2017 |
| U TAN Limited | Wholesale & retail | 2011 |
| U2TAN Limited | Other services | 2013 |
| UGO TAN Limited | Other services | 2011 |
| UGO2TAN Limited | Other services | 2011 |
| UGO4TAN Limited | Other services | 2011 |
| Ultimate Sports Goods Limited | Wholesale & retail | 2017 |
| Universal Bricks Ltd | Wholesale & retail | 2026 |
| Urban Regeneration & Development Ltd | Professional & scientific | 2025 |
| Usave Broad ST Limited | Wholesale & retail | 2021 |
| Value Heating & Plumbing Limited | Construction | 2007 |
| Vantage Signs Ltd | Other services | 2022 |
| Venue Security Services Ltd | Administrative & support | 2018 |
| VGN Bakery Co. LLP | | 2020 |
| VIP Building Services Limited | Construction | 2011 |
| VIP Nail and Beauty Limited | Other services | 2022 |
| Virtual Pro Golf Limited | Wholesale & retail | 2025 |
| Wallace GAS Services Ltd | Construction | 2012 |
| Weeks Media & Services Ltd | Transport & logistics | 2026 |
| Wembley Streamline Services Ltd | Transport & logistics | 2025 |
| Wookiee Decor Ltd | Construction | 2023 |
| Xing Hong Limited | Hospitality | 2025 |
| Xpresstan Limited | Other services | 2011 |
| Yasir Delivery Limited | Transport & logistics | 2019 |
| Zorbas Mediterranean Food Company Ltd | Hospitality | 2022 |
| Zybertrail Ltd | Wholesale & retail | 2025 |