| Accelerator BIO Consulting Ltd | Professional & scientific | 2026 |
| Andreou Sparks Ltd | Hospitality | 2014 |
| Andrew Turner Builders Limited | Construction | 2002 |
| Broto Management Ltd | Real estate | 2025 |
| Darren's Medical Ltd | Health & social care | 2024 |
| DB Technology (Cambridge) Limited | Professional & scientific | 1997 |
| DBR Data Ltd | Information & communication | 2017 |
| Dewlinks Limited | Manufacturing | 2010 |
| Digital Content Systems Limited | Information & communication | 2014 |
| Emilyhannah Ltd | Wholesale & retail | 2008 |
| Enee Design Ltd | Professional & scientific | 2018 |
| FEN END Projects C.I.C. | Other services | 2024 |
| Fullbeam European Limited | Wholesale & retail | 2006 |
| Gawn Associates Limited | Professional & scientific | 2002 |
| George Scarborough Ltd | Real estate | 2012 |
| HAZ-2-GO Ltd | Water & waste | 2005 |
| Heart Homecare Cambridge Ltd | Health & social care | 2018 |
| Hi-Biz Ltd | Manufacturing | 2007 |
| Host Healthcare Limited | Health & social care | 2024 |
| House of Prayer for ALL Nations - Europe Ltd | Other services | 2016 |
| Ingress Leak Detection Limited | Construction | 2021 |
| J P Roadways Limited | Construction | 2012 |
| J R Unwin Limited | Agriculture & fishing | 2003 |
| Jade Consultants (Cambridge) Limited | Professional & scientific | 1980 |
| Kelnetic Liberty Ltd | Financial services | 2025 |
| Kelnetic Properties Ltd | Real estate | 2023 |
| Linda Davey Limited | Other services | 2014 |
| Lorfirm Ukeig | Professional & scientific | 1993 |
| Lovefit Limited | Arts & recreation | 2011 |
| Meeting Space Limited | Professional & scientific | 2005 |
| Michelle Wilson Travel Ltd | Administrative & support | 2024 |
| Miniature Check Valves Ltd | Manufacturing | 2020 |
| NLJ Properties Limited | Construction | 2016 |
| Orca Intelligence Ltd | Professional & scientific | 2021 |
| Polarglaze Limited | Construction | 1978 |
| PRC (Cambs) Limited | Administrative & support | 2013 |
| Recruitment Hunters Limited | Administrative & support | 2017 |
| Rehab@Work Limited | Health & social care | 2005 |
| Sandy Hill Community Project Centre C.I.C. | Administrative & support | 2021 |
| Sentra Cyber Holdco Ltd | Information & communication | 2026 |
| Shastid Energy Limited | Administrative & support | 2001 |
| Smart UK Consulting Ltd | Professional & scientific | 2015 |
| SWK Maintenance Ltd | Administrative & support | 2025 |
| THE Huntingdonshire Swimming Academy LLP | | 2025 |
| THE London Business Magazine Ltd | Professional & scientific | 2024 |
| THE Product Counsel Limited | Professional & scientific | 2023 |
| THE Wineman (UK) Ltd | Wholesale & retail | 2012 |
| Treve Services Ltd | Administrative & support | 2023 |
| Tuppy Glossop Limited | Professional & scientific | 2019 |
| Version 22 Design Ltd | Manufacturing | 2012 |
| Zeoflex Limited | Manufacturing | 2000 |