| Actium Financial Advice Ltd | Real estate | 2018 |
| Alectoris Rufa Limited | Professional & scientific | 2015 |
| Automotive Restore Ltd | Wholesale & retail | 2026 |
| Blairflow Ltd | Transport & logistics | 2025 |
| Border E-Bike Hire Limited | Administrative & support | 2023 |
| Border Electronics Ltd | Wholesale & retail | 2026 |
| Borders Country Lets Ltd | Real estate | 2022 |
| C&H Winwood Catering Ltd | Hospitality | 2025 |
| Cleghorn Electrical Services Ltd | Construction | 2023 |
| Coldstream Arms Limited | Hospitality | 2016 |
| Coldstream Consultants Limited | Professional & scientific | 2018 |
| Coldstream Kebab House Ltd | Hospitality | 2026 |
| Coldstream Recycling Limited | Water & waste | 2013 |
| Coldstream Spice Ltd | Hospitality | 2025 |
| Cromar Holdings Limited | Financial services | 2015 |
| Cromar Metal Products Limited | Manufacturing | 2000 |
| D & S Innes Enterprises Ltd | Hospitality | 2021 |
| D Gibson Tree & Forestry Services Ltd | Agriculture & fishing | 2012 |
| DJX Ltd | Real estate | 2024 |
| Dwagro Limited | Professional & scientific | 2021 |
| Earth School of Higher Consciousness Ltd | Professional & scientific | 2025 |
| Eco One Electrical Ltd | Construction | 2022 |
| Family 29 Limited | Hospitality | 2025 |
| FOX Fencing (Kiwi) Limited | Construction | 2016 |
| G J Sanderson Butchers Limited | Wholesale & retail | 2026 |
| Goated Entertainment Limited | Other services | 2026 |
| GRF Farms (Ruthven) | | 1999 |
| Hhhk Ltd | Wholesale & retail | 2017 |
| Hirsel Homestead LP | | 2020 |
| Hive Gallery Ltd | Wholesale & retail | 2024 |
| Infostream Media and Publishing Limited | Information & communication | 2016 |
| Invest Partner Property Ltd | Real estate | 2025 |
| Keith Wilson Haulage Limited | Transport & logistics | 2013 |
| Kircal Woodlands Ltd | Agriculture & fishing | 2018 |
| KRJ Construction (Leitholm) Ltd | Construction | 2024 |
| Laughing Ducks Soft Play Limited | Arts & recreation | 2013 |
| Leet Financial Planning Limited | Professional & scientific | 2023 |
| Leitholm Campervans Limited | Wholesale & retail | 2023 |
| Letham Limited | Hospitality | 2008 |
| Merse & Company Limited | Professional & scientific | 1999 |
| Miller (Agricultural Sprayers) Holdings Limited | Financial services | 2025 |
| NSL Signalling Consultancy Ltd | Construction | 2015 |
| Nuvola Blue Ltd | Wholesale & retail | 2020 |
| Penelope Textiles Limited | Wholesale & retail | 2014 |
| Pittlesheugh Bess Property LLP | | 2024 |
| Pre-Landfill Solutions Ltd | Information & communication | 2026 |
| Premier Berwick Ltd | Wholesale & retail | 2024 |
| Property Maintenance Construction Ltd | Construction | 2026 |
| Proxy Scholar Ltd | Professional & scientific | 2025 |
| QVS Consultants Ltd | Information & communication | 2023 |
| R F Roofing Limited | Construction | 2014 |
| R.J. & A.C. Miller (Agricultural Sprayers) Limited | Wholesale & retail | 2003 |
| Ramsay W (Holdings) Ltd | Financial services | 2016 |
| RB Aero Services Limited | Manufacturing | 2017 |
| Remedial Property Services Ltd. | Construction | 2018 |
| RGR Trading Ltd | Wholesale & retail | 2023 |
| Ross Architectural Ltd | Professional & scientific | 2008 |
| RWS Management Services Ltd | Administrative & support | 2023 |
| S Millar & Sons Ltd | Agriculture & fishing | 2022 |
| Saris 12 Limited | Hospitality | 2025 |
| Sarj Travels Limited | Transport & logistics | 2025 |
| SC599437 Limited | Financial services | 2018 |
| Skaithmuir Farming Limited | Agriculture & fishing | 2010 |
| Sprouston Fishings LLP | | 2025 |
| Sustainability Research Services Ltd | Professional & scientific | 2025 |
| Tailored Event Services Ltd | Arts & recreation | 2025 |
| Telford Accountancy Limited | Professional & scientific | 2026 |
| THE Besom Coldstream Ltd | Hospitality | 2025 |
| THE Economic Development Academy Limited | Administrative & support | 2024 |
| THE Equestrian Jump Company LP | | 2013 |
| THE Hirsel Gallery LP | | 2017 |
| Thepaddockco Ltd | Information & communication | 2022 |
| Trees With Roots Ltd | Agriculture & fishing | 2023 |
| Tweed Leisure Limited | Hospitality | 1995 |
| Tweedbeats Limited | Agriculture & fishing | 2014 |
| Tyandlissa Ltd | Wholesale & retail | 2023 |
| Unique Renewables Limited | Professional & scientific | 2013 |
| Woodland Management Services (UK) Ltd | Administrative & support | 2012 |