| Bass Rock Consulting Limited | Professional & scientific | 2008 |
| Collective Media Limited | Information & communication | 2014 |
| Dunglass Estate Events Limited | Hospitality | 2009 |
| Evans Engineering Solutions Ltd | Construction | 2020 |
| Evolve and Emerge Limited | Professional & scientific | 2013 |
| Ferneylea Glamping Ltd | Hospitality | 2024 |
| Fulfordlees Farmers Limited | Agriculture & fishing | 2016 |
| Good Hood Homes Ltd | Real estate | 2022 |
| Greyland Energy Park Limited | Construction | 2020 |
| HAY Barn Homes Ltd | Construction | 2024 |
| John Mcgregor Consulting Limited | Professional & scientific | 2014 |
| Neep Peep Limited | Wholesale & retail | 2020 |
| Oilcustom Limited | Construction | 1998 |
| Oldhamstocks Church Trust | Other services | 2024 |
| R & C Crichton Ltd | Agriculture & fishing | 2007 |
| R & K Drysdale (Holdings) Limited | Financial services | 1996 |
| Rose & Thistle Social Ltd | Professional & scientific | 2023 |
| SN Printing Solutions Ltd | Manufacturing | 2024 |
| Spear Renovations Ltd | Construction | 2020 |
| T111 Graphics Limited | Wholesale & retail | 2025 |
| TD Tree & Land Services Limited | Agriculture & fishing | 2009 |
| THE Overlander SUB Ltd | Information & communication | 2026 |
| THE Wholebeing Project CIC | Health & social care | 2021 |
| Tower Farm Holidays LLP | | 2015 |
| Transition Energy Management Limited | Administrative & support | 2011 |
| Volta DO MAR Ltd | Financial services | 2024 |
| W.A. Gillespie & SON Limited | Construction | 1986 |
| Walking Awake Ltd | Health & social care | 2025 |
| Werc Pulse Limited | Professional & scientific | 2008 |
| West Mains Holdings Ltd | Agriculture & fishing | 2026 |
| ZOC Construction (Scotland) Ltd | Construction | 2025 |