| Eagle Studio Inc Ltd | Information & communication | 2025 |
| East West Property Holdings (Scotland) Limited | Real estate | 2024 |
| EAT Fresh Coatbridge Ltd | Hospitality | 2026 |
| EB Recruitment Ltd | Administrative & support | 2020 |
| EBS Ice Hockey Limited | Education | 2024 |
| ECO4U Installations Ltd | Construction | 2023 |
| ECO5SCOTLAND Ltd | Professional & scientific | 2025 |
| Economy Emporium Ltd | Wholesale & retail | 2024 |
| Ecoseal Home Ltd | Construction | 2025 |
| Edayr Ltd | Other services | 2024 |
| EDDIE1971 Limited | Construction | 2025 |
| Edzell 1969 Limited | Extraterritorial | 2013 |
| EEJ Ltd | Professional & scientific | 2024 |
| EFP Investments Limited | Real estate | 2016 |
| EGR Automotive Solutions Ltd | Wholesale & retail | 2025 |
| EHL Holdings Ltd | Real estate | 2015 |
| EHL Property Holdings Limited | Financial services | 2023 |
| Eighty Eight F Limited | Hospitality | 2013 |
| EK Developments Limited | Real estate | 2008 |
| Eliaa Ltd | Wholesale & retail | 2025 |
| Elite Chef Services Ltd | Hospitality | 2026 |
| Elite Clean Services (Scotland) Limited | Administrative & support | 2010 |
| Elite Golden Key Ltd | Real estate | 2025 |
| Elite Instwall Ltd | Construction | 2016 |
| Elite Joinery and Fire Protection Ltd | Construction | 2024 |
| Elite Scotland Developments Ltd | Other services | 2025 |
| Elliots News Agency Limited | Wholesale & retail | 2013 |
| Ells Rest Ltd | Real estate | 2025 |
| Elroyd Consulting Ltd | Information & communication | 2024 |
| Emajen Enterprises Ltd | Hospitality | 2026 |
| Emajen Joinery Contracts Ltd | Construction | 2020 |
| Emily Shakarji Ltd | Real estate | 2018 |
| Empire Barber CUT Ltd | Other services | 2022 |
| Empower Care Scotland Ltd. | Health & social care | 2000 |
| Endoscot Limited | Health & social care | 2019 |
| Enercool Climate Solutions Ltd | Construction | 2019 |
| Energy Repair Team Ltd | Construction | 2016 |
| Energy Resource Solutions Ltd. | Other services | 2012 |
| Engage Recruitment Limited | Administrative & support | 2017 |
| Enquire Investments Ltd | Real estate | 2024 |
| Enquire Property Ltd | Real estate | 2023 |
| EO Asbestos Specialists Ltd | Professional & scientific | 2020 |
| Eprotek PCB Solutions Limited | Manufacturing | 2021 |
| Ereventis Ltd | Professional & scientific | 2026 |
| ERG (Central Scotland) Ltd | Construction | 2022 |
| Erilyn Ltd | Households | 2025 |
| ERP Property Group Ltd | Extraterritorial | 2020 |
| Escj Contracts Limited | Construction | 2017 |
| ESS Bookkeeping Ltd | Professional & scientific | 2025 |
| Essbee Coaches Limited | Transport & logistics | 1999 |
| Euro Hydraulics Scotland Holdings Limited | Financial services | 2023 |
| Euro Hydraulics Scotland Limited | Wholesale & retail | 2000 |
| Euro Japs Scotland Ltd | Wholesale & retail | 2017 |
| Eurojaps Salvage Ltd | Wholesale & retail | 2025 |
| European Minimart Ltd | Wholesale & retail | 2023 |
| Evergreen Store Limited | Wholesale & retail | 2026 |
| Everpris African Shop Ltd | Wholesale & retail | 2021 |
| Exmel Solutions Limited | Wholesale & retail | 2008 |
| Express Yourself NOW Ltd | Education | 2025 |
| Extra Gleam Cleaning Limited | Administrative & support | 2023 |
| Eyebolls Limited | Information & communication | 2015 |
| F M Roofing Solutions Ltd | Construction | 2026 |
| F&A Building Services Limited | Construction | 2017 |
| F.M.T.C Limited | Professional & scientific | 2023 |
| Facility Pro Electrical Services Scotland Limited | Construction | 2025 |
| Fameuk Ltd | Wholesale & retail | 2025 |
| Fastfix Electrix Ltd | Construction | 2025 |
| Faulds Homes Limited | Households | 2022 |
| FD Claims Ltd | Other services | 2024 |
| FG MK2 Limited | Hospitality | 2023 |
| Fibretech Solutions Ltd | Construction | 2025 |
| Fife Silica Sands Limited | Extraterritorial | 2002 |
| Fifth Gear Limited | Administrative & support | 2003 |
| Finance Assistance Ltd | Professional & scientific | 2013 |
| Finance First & Accountancy Services Limited | Professional & scientific | 2023 |
| Financial Compensation Centre Limited | Financial services | 2003 |
| Firefight Productions Ltd | Arts & recreation | 2026 |
| Firehouse Grill Ltd | Hospitality | 2019 |
| Firstcoder.Com Group Ltd | Manufacturing | 2017 |
| Fixit Facilities Ltd | Other services | 2025 |
| Fixora Repairs Ltd | Other services | 2025 |
| Flametec Heating Ltd | Construction | 2024 |
| Flawless Limited | Administrative & support | 2026 |
| Fleet Automotive P/S Scotland Limited | Extraterritorial | 2020 |
| Fleet Automotive Scotland Limited | Wholesale & retail | 2017 |
| Flexible Healthcare One Solutions Ltd | Administrative & support | 2015 |
| Fmck Developments Ltd | Other services | 2026 |
| Forsyth Mechanical and Electrical Ltd | Construction | 2024 |
| Forward Security Solutions Limited | Administrative & support | 2026 |
| Fountain Bar (Coatbridge) Limited | Hospitality | 2017 |
| FP Business Services Limited | Professional & scientific | 2009 |
| Fraser Fire Alarms Limited | Extraterritorial | 2008 |
| Fraser Haulage Limited | Transport & logistics | 1986 |
| Fraser Holdings Limited | Transport & logistics | 1986 |
| Frontline Consultants Limited | Professional & scientific | 1990 |
| Full Insight Ltd. | Other services | 2017 |
| G & T Sports Limited | Hospitality | 2015 |
| G & W Tiling Ltd | Construction | 2024 |
| G&J Joinery and Building Services Ltd | Construction | 2024 |
| G.K.A Specialist Consultants Ltd | Construction | 2024 |