| AJ Signalling Ltd | Construction | 2022 |
| Another Lane EN Limited | Financial services | 2011 |
| Ashwood Business Services Limited | Administrative & support | 2013 |
| Axis Project Management Limited | Construction | 2008 |
| B & S Electrical Services Ltd | Construction | 2018 |
| Big Indie Limited | Administrative & support | 2016 |
| BIO Decontamination Ltd | Professional & scientific | 2019 |
| Blackspot Holdings Ltd | Financial services | 2024 |
| Blaxton Property Services Ltd | Construction | 2014 |
| Brick Products Limited | Manufacturing | 2002 |
| Browns Construction Services Ltd | Construction | 2019 |
| Cayley Court Management Company Limited | Households | 2002 |
| Chris Coetzee Fencing Limited | Other services | 2015 |
| CMS Staycations Limited | Hospitality | 2020 |
| Collimoore Assets and Estates Ltd | Real estate | 2026 |
| Comewel Limited | Professional & scientific | 2015 |
| Contact Sezc Ltd | Professional & scientific | 2023 |
| Crown Solicitors Limited | Professional & scientific | 2012 |
| CTT Logistics Ltd | Manufacturing | 2018 |
| DC Training & Development Services Limited | Administrative & support | 2002 |
| DH Golf & Performance Limited | Arts & recreation | 2018 |
| Direct Rail Solutions Ltd | Construction | 2015 |
| Dragonfly Acoustics Limited | Professional & scientific | 2007 |
| E.A. Gibbs & SON Limited | Construction | 1961 |
| Ebor Systems Ltd | Information & communication | 1996 |
| Exelus Consulting Limited | Professional & scientific | 2011 |
| Farrell Business Solutions Limited | Professional & scientific | 2013 |
| Firebox.Com Limited | Wholesale & retail | 1999 |
| Fletcher Farming and Dairy Services Limited | Agriculture & fishing | 2017 |
| Full Circuit Group Ltd | Construction | 2011 |
| Getting There Groundworks Ltd | Administrative & support | 2018 |
| Gifting Monkeys Ltd | Manufacturing | 2021 |
| GPS Services Doncaster Ltd | Manufacturing | 2021 |
| Grey Wolf Services Ltd | Professional & scientific | 2020 |
| Growth and Airway Ltd | Health & social care | 2026 |
| Hayes Construction York Ltd | Construction | 2021 |
| Henry Thacker Limited | Administrative & support | 2011 |
| HI Form Equine Ltd | Wholesale & retail | 2017 |
| Horticulinary Ltd | Hospitality | 2024 |
| Houz of Harrogate Ltd | Wholesale & retail | 2013 |
| Impress Construction Limited | Construction | 2015 |
| Industrial Doors R US Limited | Financial services | 2012 |
| Invest and Gift Limited | Financial services | 2013 |
| Isaac Poad Brewing Ltd | Manufacturing | 2016 |
| IT Project Experts Limited | Information & communication | 2013 |
| IWP Inc Ltd | Professional & scientific | 2017 |
| J G Windows (York) Limited | Construction | 2018 |
| J Rowe Enterprises Ltd | Wholesale & retail | 2018 |
| J W Enterprises (York) Ltd | Hospitality | 2023 |
| Jfeathers Active Recruitment Ltd | Administrative & support | 2024 |
| Jwpcreers LLP | | 2011 |
| Kepwick Design Limited | Information & communication | 1995 |
| King and Captain Ltd | Other services | 2017 |
| Kmel (UK) Ltd | Hospitality | 2016 |
| KPM Trading Limited | Hospitality | 2013 |
| Land Innovations Limited | Administrative & support | 2014 |
| Leapfrog Recruitment Ltd | Administrative & support | 2016 |
| LEO Interiors Ltd | Construction | 2018 |
| Little Acorns (Clifton Green) Limited | Health & social care | 2011 |
| Little Motel Motion Studio Ltd | Information & communication | 2015 |
| LJW Construction Ltd | Construction | 2002 |
| LN Home Improvements Ltd | Water & waste | 2022 |
| Loaf Marketing & Media Ltd | Professional & scientific | 2023 |
| Local Properties (Pontefract) Limited | Real estate | 2011 |
| LW Services Ltd | Manufacturing | 2021 |
| Malton Transport Services Limited | Transport & logistics | 2005 |
| Manga Energy Limited | Energy | 2016 |
| Meditemp Limited | Administrative & support | 2015 |
| MR Andrews Online Limited | Education | 2013 |
| Mygloe Limited | Information & communication | 2015 |
| Nash Groundworks Ltd | Construction | 2018 |
| Nunns Enterprises Ltd | Financial services | 2021 |
| Oakwood Park Homes Limited | Manufacturing | 2014 |
| Oliver Excavations Limited | Construction | 2015 |
| Olivers Design House Limited | Construction | 2014 |
| Orangery Solutions Limited | Construction | 2010 |
| Peerless Hygiene Solutions Ltd | Wholesale & retail | 2014 |
| Planning Gain Experts (Training Academy) Limited | Administrative & support | 2022 |
| Planning Gain Experts Ltd | Real estate | 2021 |
| PLM Trading Limited | Hospitality | 2016 |
| Plush Café Ltd | Hospitality | 2019 |
| Professional Recruiters Limited | Administrative & support | 2008 |
| Project 2017 Limited | Real estate | 2017 |
| Rare Recycling Limited | Water & waste | 2015 |
| Rasmoco Ltd | Wholesale & retail | 2022 |
| Regulatory Solutions Consulting Ltd | Administrative & support | 2011 |
| Rideal Associates Ltd | Professional & scientific | 2017 |
| ROC Properties Limited | Real estate | 2007 |
| Roger Petch Transport Limited | Transport & logistics | 2010 |
| Rose Centre Business Park Limited | Construction | 2012 |
| Royal Oak Ripon Ltd | Hospitality | 2013 |
| S&A+ Ltd. | Professional & scientific | 2019 |
| Saftem Business and Safety Services Limited | Other services | 2009 |
| Sanders Melber Limited | Real estate | 2007 |
| SBR Heating Services Ltd | Construction | 2015 |
| Spmi Consulting Ltd | Administrative & support | 2017 |
| Sssurgery Ltd | Health & social care | 2019 |
| Taylor ATV Limited | Wholesale & retail | 2011 |
| THE Caulfield Conservatory Company Limited | Construction | 2001 |
| THE Flamingo Corporation Ltd | Hospitality | 2023 |