| Twentythreehundred Limited | Professional & scientific | 2018 |
| Twin Trees Properties Limited | Hospitality | 2017 |
| Tygerberg Limited | Professional & scientific | 2010 |
| Tylgarth Services Limited | Professional & scientific | 2003 |
| U Save Automotive Limited | Transport & logistics | 1999 |
| Ubicoat Limited | Professional & scientific | 2014 |
| UK Detailing Academy Ltd. | Education | 2017 |
| UK Detailing Limited | Wholesale & retail | 2011 |
| UK Planning Studio Ltd | Professional & scientific | 2019 |
| Ukko Organics Limited | Agriculture & fishing | 2019 |
| Uktt Limited | Education | 2015 |
| Ulterior Tech Limited | Information & communication | 2010 |
| Ultimate Renaissance Limited | Wholesale & retail | 1999 |
| Uncommon Distillery Limited | Extraterritorial | 2024 |
| Unicosystems Ltd | Construction | 2024 |
| Unyvation Limited | Professional & scientific | 2026 |
| Upper Coln Farm and Stud Limited | Agriculture & fishing | 1993 |
| Upshift Consultancy Ltd | Manufacturing | 2020 |
| Urbana Property Ltd | Construction | 2023 |
| Urza Ltd | Wholesale & retail | 2025 |
| Usthree Group Holdings Ltd | Professional & scientific | 2015 |
| V.I.Global Ltd | Financial services | 2012 |
| V12 Telecom Limited | Information & communication | 1998 |
| Valka Enterprises Ltd | Agriculture & fishing | 2026 |
| Value Holdings Limited | Extraterritorial | 2025 |
| Valuematrix Limited | Professional & scientific | 2024 |
| Varg Group Limited | Information & communication | 2016 |
| Vaykay Ltd | Real estate | 2020 |
| Vector Talent Limited | Administrative & support | 2020 |
| Velocitude Events Ltd | Extraterritorial | 2025 |
| Verdicotts Creative Services LLP | | 2021 |
| Veritas Coaching Global Ltd | Health & social care | 2023 |
| Veritas Finance Services Limited | Professional & scientific | 2019 |
| Veritas Hotels Ltd | Financial services | 2016 |
| Veronika International Ltd | Extraterritorial | 2022 |
| Vertex Executive Ltd | Professional & scientific | 2017 |
| Vertigrip Fitness Ltd | Manufacturing | 2025 |
| Vetsbarn Limited | Professional & scientific | 2026 |
| Viadi Services (UK) Limited | Administrative & support | 2023 |
| Vianexus Purpose Ltd | Information & communication | 2025 |
| Villa 66 Limited | Construction | 2021 |
| Vince Janes Ltd | Agriculture & fishing | 2013 |
| Virtual CFO Ltd | Professional & scientific | 2010 |
| Vision Wave Limited | Information & communication | 2003 |
| Voga Interiors Ltd | Construction | 2015 |
| Voices Together Group Limited | Arts & recreation | 2021 |
| Volcanic Investments Limited | Financial services | 2013 |
| Voler Aviation Services Ltd | Transport & logistics | 2014 |
| Vortal Homes Limited | Other services | 2017 |
| W. Roberts Transport Limited | Information & communication | 2003 |
| Wacd Property Group Ltd | Real estate | 2025 |
| Waddell Capital Ltd | Real estate | 2026 |
| Wakefield James Ltd | Professional & scientific | 2024 |
| Wakfer Limited | Financial services | 2001 |
| Walter Bull & SON (Cirencester) Limited | Wholesale & retail | 1951 |
| Warrens Gorse Mews Management Company Limited | Real estate | 2013 |
| Waterland Gear Limited | Arts & recreation | 1988 |
| Waterloo Cleaners Ltd | Other services | 2023 |
| Waterloo Letting Limited | Households | 2025 |
| Watermark Building Consultants Limited | Construction | 2009 |
| Watermark Cotswolds Limited | Professional & scientific | 1997 |
| Watermark Developments Limited | Real estate | 2005 |
| Watermark Leisure and Property Group Limited | Professional & scientific | 2004 |
| Watermark Management Company Limited | Professional & scientific | 2005 |
| Watermoor House R C H | Health & social care | 2014 |
| Watermoor Point Ltd | Real estate | 2021 |
| Waterwell Limited | Construction | 1998 |
| Ways With Words Translation Services Ltd | Professional & scientific | 2009 |
| WB Capital Ventures Limited | Construction | 2024 |
| WBM Commercial Property Limited | Real estate | 2013 |
| WDV Roofing and Property Maintenance Limited | Construction | 2025 |
| WE Love 9AM Limited | Information & communication | 2016 |
| Wealth and Home Limited | Professional & scientific | 2018 |
| Wearmode Limited | Wholesale & retail | 1983 |
| Web & Digital Marketing Solutions Ltd | Manufacturing | 2013 |
| Webb and Jeffery Farming Ltd | Agriculture & fishing | 2011 |
| Webbs Garage South Cerney Ltd | Wholesale & retail | 2023 |
| Weekend Candy Limited | Professional & scientific | 2012 |
| Welcome Health Ventures Ltd | Professional & scientific | 2024 |
| Well Deep Therapy Ltd | Wholesale & retail | 2025 |
| Well Hill Kitchens Limited | Hospitality | 2015 |
| Wellspring Safety Limited | Professional & scientific | 2002 |
| West Bar Developments Limited | Real estate | 2003 |
| West Lodge (Cirencester) Management Company Limited | Households | 2005 |
| West London Pet Services Ltd | Other services | 2022 |
| Westcon Group Africa Operations Limited | Information & communication | 1976 |
| Westcon Group European Operations Limited | Information & communication | 2002 |
| Westcon Group Middle East Holdings Limited | Information & communication | 2010 |
| Westcon International Group Holdings Limited | Information & communication | 2003 |
| Westcon International Limited | Information & communication | 2002 |
| Weston Mitchell Limited | Wholesale & retail | 1986 |
| Whelford Properties Limited | Professional & scientific | 2006 |
| Wheretostay UK Limited | Administrative & support | 2013 |
| WHG Privacy Ltd | Extraterritorial | 2023 |
| While at the Waterside Limited | Hospitality | 2023 |
| Whitaker and Seager Properties Limited | Real estate | 2019 |
| Whitehall Advisers Limited | Professional & scientific | 2003 |
| Whiteway Rocket Limited | Financial services | 2021 |
| Wickstead Horseplay Ltd | Agriculture & fishing | 2013 |
| Wild & Angell Consulting Ltd | Professional & scientific | 2025 |