| R Howell Building Services Ltd | Construction | 2012 |
| R&C Cooke Limited | Financial services | 2022 |
| RAM Property Developments Ltd | Real estate | 2020 |
| Ramptine Limited | Professional & scientific | 2016 |
| Ravenstone (Chalford) Management Limited | Households | 2005 |
| RAW Sports Limited | Wholesale & retail | 1996 |
| RCL Electrical Services (UK) Limited | Construction | 2009 |
| Recycleline Limited | Transport & logistics | 2020 |
| Redivider Limited | Professional & scientific | 2018 |
| Redz Hairdressing Ltd | Other services | 2025 |
| Regal Rims Ltd | Wholesale & retail | 2026 |
| Regent Street Garage Limited | Wholesale & retail | 2009 |
| Rella Limited | Administrative & support | 2024 |
| Resolver Consultancy Ltd | Professional & scientific | 2020 |
| Retro EV Ltd | Manufacturing | 2017 |
| Retrospect Holdings Ltd | Financial services | 2024 |
| Rhoades Young Design Limited | Professional & scientific | 2006 |
| Rialta Holdings Limited | Real estate | 2018 |
| Richard Butler (SW) Ltd | Real estate | 2025 |
| Riddle (Holdings) Limited | Households | 1999 |
| Risk and Security Solutions Ltd | Professional & scientific | 2024 |
| River and Ridge Ltd | Arts & recreation | 2010 |
| RMC Group Ltd | Construction | 2015 |
| Roaming Hemispheres Limited | Extraterritorial | 2025 |
| ROB Hughes Installations Ltd | Construction | 2013 |
| RON Martin (Ross Building) Limited | Construction | 2001 |
| Ross Firewood Company Ltd | Agriculture & fishing | 2022 |
| Ross Pastry Products Ltd | Hospitality | 2022 |
| Rothdean Limited | Manufacturing | 1972 |
| Roya 2015 Limited | Financial services | 2015 |
| RSA Bennett Ltd | Real estate | 2021 |
| Ryeford Property Limited | Real estate | 2024 |
| Rythmico Ltd | Education | 2021 |
| S & E Carter Investments Ltd | Real estate | 2017 |
| S. Wolf Estates Limited | Real estate | 1996 |
| S.J.Harris Ltd | Construction | 2011 |
| SA Builders Limited | Construction | 2002 |
| Sampson Commercial Properties Ltd | Construction | 2026 |
| Satch's Snacks Limited | Wholesale & retail | 2022 |
| SCG IFA Ltd | Financial services | 2016 |
| Schmooly Limited | Professional & scientific | 2017 |
| Scoff's Coffee House Limited | Hospitality | 2020 |
| Scuff Solutions Ltd | Wholesale & retail | 2019 |
| SD & CY Holdings Limited | Real estate | 2016 |
| Sea Eagles Retail Ltd | Wholesale & retail | 2026 |
| Seal Glass Limited | Administrative & support | 2000 |
| Seal Lettings Limited | Real estate | 2023 |
| Searancke Properties Limited | Real estate | 2019 |
| Sebbie-J Ltd | Real estate | 2022 |
| Seby Services Ltd | Construction | 2016 |
| Seeking Sense and Science Limited | Information & communication | 2015 |
| Send for Families Ltd | Education | 2026 |
| Serg Carpentry Limited | Construction | 2011 |
| Severn Dean Support Ltd | Health & social care | 2011 |
| Severn Ready Space Ltd | Extraterritorial | 2011 |
| Severnside Relocatable Systems Limited | Wholesale & retail | 1997 |
| Shergill One Ltd | Wholesale & retail | 2021 |
| Shire Fuels Ltd | Wholesale & retail | 2021 |
| Sitatunga Limited | Professional & scientific | 1989 |
| SJS Book Keeping & Accountancy Services Limited | Professional & scientific | 2022 |
| SJW Consultancy Ltd | Financial services | 2016 |
| Slabs 2 U Limited | Manufacturing | 2009 |
| SMR Motors Southwest Ltd | Wholesale & retail | 2025 |
| Somerwood Designs Group Limited | Financial services | 2026 |
| Somerwood Designs Limited | Wholesale & retail | 2012 |
| Sonic Industries Limited | Information & communication | 2024 |
| Sorella Hair Boutique Ltd | Other services | 2019 |
| SOS D&C Cleaning Services Ltd | Administrative & support | 2026 |
| SOS Scrap Ltd | Water & waste | 2026 |
| Sosgas Ltd | Construction | 2026 |
| Soudley Fishing Syndicate Limited | Arts & recreation | 1950 |
| Speedwerks Ltd | Wholesale & retail | 2019 |
| Speedy Feet Ltd | Wholesale & retail | 2015 |
| Sprung Suspension Workshop Ltd | Manufacturing | 2015 |
| Squiff Limited | Professional & scientific | 2006 |
| SRS Cabins Ltd | Extraterritorial | 2011 |
| ST White’S Developments Limited | Construction | 2017 |
| Stellatum Ltd | Other services | 2013 |
| Stem Financial Ltd | Financial services | 2015 |
| Stock Excavators Limited | Manufacturing | 2016 |
| Stock Group Limited | Financial services | 2018 |
| Stock Group Properties Limited | Real estate | 2025 |
| Stock Re-Manufacturing Limited | Manufacturing | 2015 |
| Stock Sweepers Limited | Manufacturing | 2007 |
| Stocks (UK) Limited | Manufacturing | 2007 |
| Stocks Innovations Limited | Professional & scientific | 2014 |
| Sublime Heritage Ltd | Construction | 2025 |
| Success in Plastics Engineering Ltd | Manufacturing | 2008 |
| SUP Gloucester Ltd | Arts & recreation | 2016 |
| Sylvan Cottages Ltd | Real estate | 2018 |
| T & L Optics Limited | Health & social care | 2006 |
| T.H.Haskett & SON Limited | Construction | 1960 |
| Taag Private Ltd | Wholesale & retail | 2024 |
| Taste of Romania Market Ltd | Wholesale & retail | 2026 |
| TDP Engineering Ltd | Manufacturing | 2025 |
| Temco Wire Products Limited | Manufacturing | 1947 |
| Tenancy Assured Ltd | Real estate | 2026 |
| Tfrf Ltd | Professional & scientific | 2023 |
| THE Bespoke Brewing Co. Limited | Manufacturing | 2012 |
| THE Chippy Cinderford Ltd | Hospitality | 2024 |