| Dalcin Barbershop Ltd | Other services | 2021 |
| DAN Bizzarro Method Ltd | Education | 2026 |
| Daraja Arts Limited | Wholesale & retail | 2007 |
| Darren Freeman Limited | Professional & scientific | 2012 |
| Dash Grange Stud Limited | Agriculture & fishing | 2021 |
| Davenport Close Management Company Limited | Households | 2011 |
| David Armfield Limited | Professional & scientific | 2010 |
| David Law Land Agency Limited | Real estate | 2019 |
| DAY2DAY Limited | Agriculture & fishing | 1999 |
| DC Automotive Car Sales Limited | Wholesale & retail | 2014 |
| DCS Group Holdings Limited | Real estate | 2001 |
| DCS Inc Limited | Financial services | 2010 |
| DE Vater & Co Limited | Other services | 2015 |
| Dean and Chadlington Summer Music Festival | Arts & recreation | 2007 |
| Dean Mill Investments Limited | Professional & scientific | 2017 |
| Deanmor Investments | Financial services | 2021 |
| Decoris Home Limited | Professional & scientific | 2023 |
| Delicious Designs Ltd | Wholesale & retail | 2012 |
| Designbrown Limited | Information & communication | 1999 |
| Detail Cleaning Ltd | Extraterritorial | 2022 |
| Develop Inclusion Limited | Professional & scientific | 2022 |
| DG Associates Ltd | Professional & scientific | 2024 |
| DGL Partners Limited | Financial services | 2024 |
| Diamond Fuel Supplies Limited | Wholesale & retail | 1980 |
| Digital DRS Limited | Information & communication | 2021 |
| DIO Davies Ltd | Wholesale & retail | 2016 |
| Dipak Kalra Limited | Health & social care | 2008 |
| Direct TM Ltd | Construction | 2025 |
| Ditchley Foundation(The) | Education | 1958 |
| Ditchley Park Conference Centre Limited | Other services | 1985 |
| DOJO25 Limited | Transport & logistics | 2024 |
| Donica Publishing Ltd. | Information & communication | 1996 |
| Dopara Group Ltd | Information & communication | 2026 |
| Dore Brickwork Ltd | Construction | 2025 |
| Dore Plumbing and Heating Ltd | Construction | 2022 |
| Dovetail Carpentry & Installations Ltd | Manufacturing | 2022 |
| Doxa Wine Limited | Manufacturing | 2020 |
| DR Edwina Thompson & Associates Limited | Professional & scientific | 2010 |
| DR Lara Watson Ltd | Health & social care | 2020 |
| DR Malcolm W Brown Digital Health Medical Affairs Ltd | Health & social care | 2025 |
| Drella Productions Limited | Information & communication | 2023 |
| Drive With Manuela Limited | Education | 2026 |
| Drone Football Limited | Education | 2025 |
| DSA Project Management Limited | Professional & scientific | 2021 |
| Dulwich Acupuncture Limited | Wholesale & retail | 2006 |
| Duncan Threadgould Consulting Limited | Information & communication | 2011 |
| Dunstan Builders Ltd | Extraterritorial | 2010 |
| Duralock (UK) Limited | Manufacturing | 1991 |
| E-Experience Limited | Wholesale & retail | 2000 |
| Early Years Klique Ltd | Information & communication | 2025 |
| Eazypost Limited | Extraterritorial | 2017 |
| EB Academy Landscaping and Maintenance Ltd | Administrative & support | 2026 |
| Echo-Sunpower Solutions Limited | Professional & scientific | 2026 |
| Eclipse Auctions Limited | Wholesale & retail | 2012 |
| Eclipse Auctions Property Limited | Extraterritorial | 2023 |
| Eclipse Corporate Finance Limited | Financial services | 2019 |
| Eclipse Equity Limited | Extraterritorial | 2022 |
| Economed Limited | Manufacturing | 2020 |
| Editions Systeme D Ltd | Information & communication | 2012 |
| Effective Command Ltd | Information & communication | 2022 |
| Eldon Accountants Limited | Financial services | 1999 |
| Electronic Dream Plant Limited | Professional & scientific | 2021 |
| Elite Autos Oxford Ltd | Wholesale & retail | 2025 |
| Elizabeth Holloway Communications Limited | Professional & scientific | 2020 |
| ELM Crescent Ltd | Real estate | 2013 |
| ELM Tree and Partners Limited | Manufacturing | 2009 |
| Elmsfield Management Company Limited | Real estate | 2000 |
| Elmstead Management Limited | Households | 2019 |
| Elsa Ducker Coaching Limited | Administrative & support | 2009 |
| EME Trading Ltd | Wholesale & retail | 2017 |
| Emerita Limited | Professional & scientific | 2002 |
| Emma Brooks Homes Ltd | Real estate | 2020 |
| EN Todo Ltd | Professional & scientific | 2016 |
| Energise Brand Communications Limited | Administrative & support | 1996 |
| ENJ Construction Ltd | Construction | 2024 |
| Enstone Sales & Services Limited | Administrative & support | 2009 |
| Entodo Party Wall Surveyors Limited | Professional & scientific | 2021 |
| Equal Partners UK Limited | Financial services | 2000 |
| Equilibrium Wellness Limited | Other services | 2023 |
| Ernigrip Motors Limited | Wholesale & retail | 2003 |
| Essentium Ltd | Professional & scientific | 2011 |
| Estone FOX Limited | Administrative & support | 2022 |
| Euro Investments (Properties) Limited | Real estate | 1985 |
| Evade Limited | Wholesale & retail | 2015 |
| Evenlode Analytics Ltd | Information & communication | 2025 |
| Evenlode Cottages Management Company Limited | Households | 2006 |
| Evenlode Landscape Recovery Limited | Agriculture & fishing | 2024 |
| Evenlode Vale Ministry Trust | Other services | 1994 |
| Evenlode View (Management Company) Limited | Households | 2022 |
| Evergreen Properties Limited | Real estate | 1970 |
| Evernutra Foods Ltd | Wholesale & retail | 2025 |
| Evernutra Properties Ltd | Real estate | 2025 |
| Eversley Lane Management Company Limited | Households | 2021 |
| Exclusive Property Limited | Real estate | 2017 |
| Exclusive Travel Limited | Administrative & support | 2024 |
| Express Builders (Cotswolds) Limited | Construction | 2012 |
| Ext.Day Ltd | Information & communication | 2024 |
| Extraloo Limited | Administrative & support | 2018 |
| EYE SPY Land Limited | Real estate | 2025 |
| Eynsham Press Limited | Real estate | 1992 |