| Snse Trustee Company Limited | Other services | 2023 |
| Snsed Limited | Real estate | 2021 |
| Snsem Limited | Mining & quarrying | 2021 |
| SNSEM2 Limited | Mining & quarrying | 2021 |
| Snser Limited | Real estate | 2021 |
| SNSER2 Limited | Real estate | 2021 |
| SNSER3 Limited | Real estate | 2021 |
| Solid Structures (UK) Limited | Professional & scientific | 2005 |
| SOLVE3 Limited | Professional & scientific | 2012 |
| Somasynthesis Ltd | Other services | 2021 |
| Sonar Intelligence Ltd | Information & communication | 2012 |
| Sonic Padel Limited | Education | 2025 |
| Sonnox Limited | Information & communication | 2006 |
| Soon Erased Ltd | Wholesale & retail | 2025 |
| Sorbus Management Limited | Financial services | 2019 |
| Sorrel One Limited | Health & social care | 2021 |
| Source Music Services Ltd | Information & communication | 2020 |
| South Coast Masts and Rigging Ltd | Financial services | 2021 |
| Southcombe Garage Holdings Limited | Professional & scientific | 2011 |
| Southcombe Garage Limited | Wholesale & retail | 2002 |
| SOW to Reap Limited | Professional & scientific | 2026 |
| SP Property Maintenance Limited | Construction | 2010 |
| Sparkly Clean Cotswolds Limited | Administrative & support | 2025 |
| Specialeffect.Org.Uk Limited | Arts & recreation | 2007 |
| Spellman Productions Limited | Information & communication | 2004 |
| Splendid Interiors Ltd. | Wholesale & retail | 1994 |
| Spotty Dog Post Ltd | Information & communication | 2020 |
| Spring Park Investments Limited | Financial services | 2017 |
| SRC Contracting Ltd | Construction | 2025 |
| Ssmi Property Ltd | Real estate | 2025 |
| ST Petrocs (Trebetherick) Limited | Construction | 2023 |
| Stadiaka Capital LLP | | 2025 |
| Start-Hub Ltd | Information & communication | 2026 |
| Stebbings Limited | Professional & scientific | 2003 |
| Steele Moon Films Limited | Arts & recreation | 2016 |
| Steve O'Reilly Lighting & Electrical Services Ltd | Construction | 2003 |
| Stone Barn Fitness Limited | Arts & recreation | 2014 |
| Stoneletters Limited | Manufacturing | 2008 |
| Stormfront Film Limited | Information & communication | 2025 |
| Structural & Building Design Consultants Limited | Professional & scientific | 2020 |
| Structure Craft Limited | Construction | 2013 |
| Studlands House Ltd | Hospitality | 2019 |
| Style Street Limited | Other services | 2021 |
| Sunley Events Limited | Administrative & support | 2017 |
| Sunny Oaks Renewable Energy Park Ltd | Energy | 2022 |
| Supersaurs Limited | Other services | 2011 |
| Supreme Gourmet Events Limited | Hospitality | 2022 |
| Surepride Limited | Wholesale & retail | 2005 |
| Surtees Furniture Ltd | Manufacturing | 2017 |
| Swailbrook Place Management Ltd | Real estate | 2018 |
| Sweetpeas of Great TEW Limited | Wholesale & retail | 2009 |
| Swift Venue Finders Limited | Administrative & support | 2007 |
| Sympathetic Services Ltd | Professional & scientific | 2026 |
| Synergi Europe Limited | Wholesale & retail | 2016 |
| T&T Worldwide Ltd | Wholesale & retail | 2020 |
| Tactical Tuition Limited | Education | 2017 |
| Talia Cobbold Ltd | Professional & scientific | 2018 |
| TAP Dancing Crocodile Productions Ltd | Information & communication | 2023 |
| Tapback Ltd | Information & communication | 2026 |
| Tarquin Holdings Limited | Financial services | 2026 |
| Tax Communications Limited | Information & communication | 2018 |
| TDR Driving School Limited | Education | 2012 |
| Team Pics Ltd | Professional & scientific | 2023 |
| Team Tea Ltd | Manufacturing | 2017 |
| TED George Limited | Real estate | 2022 |
| Teddy Tennis Limited | Wholesale & retail | 2002 |
| Templar Cctv Ltd | Administrative & support | 2019 |
| Temple Oaks Renewable Energy Park Ltd | Energy | 2022 |
| Templeco 374 Limited | Agriculture & fishing | 1997 |
| Templeton Dental Ltd | Health & social care | 2022 |
| TEN Teas Ltd | Wholesale & retail | 2024 |
| TGB Consultancy Ltd | Administrative & support | 2018 |
| Thames Valley Tree Care Ltd | Agriculture & fishing | 2015 |
| THE Agricultural Lime Company Ltd | Other services | 2025 |
| THE Atacama Trucking Company Limited | Professional & scientific | 2005 |
| THE Basket Room Limited | Wholesale & retail | 2018 |
| THE Charlbury Flower Co. Ltd | Wholesale & retail | 2016 |
| THE Chequers Inn Churchill Limited | Hospitality | 2017 |
| THE Chillford Company Limited | Professional & scientific | 2003 |
| THE Chippy Larder CIC | Hospitality | 2022 |
| THE Cinnamon Stick Ltd | Hospitality | 2017 |
| THE Concierge Connection Ltd | Administrative & support | 2021 |
| THE Copy Agency Ltd | Information & communication | 2020 |
| THE Cotswold (Britannia) Ltd | Hospitality | 2026 |
| THE Cotswold AI Company Limited | Information & communication | 2025 |
| THE Cotswold Bathroom Company Limited | Construction | 2015 |
| THE Cotswold Cleaning Company Ltd | Administrative & support | 2021 |
| THE Cotswold CUP Equestrian LLP | | 2021 |
| THE Cotswold Illustrated Company Limited | Wholesale & retail | 2022 |
| THE Cotswold Project Holistic CIC | Education | 2023 |
| THE Cottage Retreat Limited | Real estate | 2025 |
| THE Curiosities Company Ltd | Manufacturing | 2017 |
| THE Easy Button Ltd | Information & communication | 2025 |
| THE Economic Brief | | 1977 |
| THE Fibre Works Limited | Wholesale & retail | 2011 |
| THE Fleet Manager Limited | Administrative & support | 2013 |
| THE Founder and ME Limited | Professional & scientific | 2023 |
| THE Founders Network Group Ltd | Other services | 2023 |
| THE FOX MB Ltd | Wholesale & retail | 2026 |
| THE Fundraising Practice Limited | Administrative & support | 2011 |