| 110 Dental Limited | Health & social care | 2013 |
| 1ST Packaging Ltd | Manufacturing | 2008 |
| 2GUYZ Pizza LLP | | 2025 |
| 2TI Limited | Information & communication | 2005 |
| 360 Event Consulting Limited | Professional & scientific | 2011 |
| 3D Tool Design and Components Limited | Professional & scientific | 2015 |
| 3SIXTY Event Consulting Limited | Professional & scientific | 2017 |
| 5TH Day Ltd | Professional & scientific | 2018 |
| 8 Blackwater Road Residents Limited | Households | 2018 |
| 8IGHTY 9INE Ltd | Financial services | 2022 |
| A J Print & Promotional Services Ltd | Manufacturing | 2020 |
| A Stallwood Limited | Construction | 2012 |
| A. D. and W. C. Lixton | | 1976 |
| A. J. Orpin Carpentry Ltd | Manufacturing | 2021 |
| ABS Consultants Ltd | Professional & scientific | 2019 |
| Ace 121 Tuition Limited | Education | 2026 |
| Ace Group Tuition Limited | Education | 2026 |
| ACJ Mechanical Ltd | Professional & scientific | 2023 |
| Active Surface Technology UK Limited | Administrative & support | 2003 |
| Adaptive Electrical Ltd | Construction | 2004 |
| Adcram Solutions Ltd | Wholesale & retail | 2009 |
| Adnora Limited | Information & communication | 2025 |
| Aerial Records Limited | Arts & recreation | 2018 |
| After Life Loans Limited | Financial services | 2025 |
| AGB F Holdings Ltd | Real estate | 2025 |
| Agem Catering Limited | Hospitality | 2022 |
| Ahkj Limited | Financial services | 2023 |
| Airtime Ltd | Administrative & support | 2023 |
| Ajce Developments Limited | Construction | 2025 |
| Ajpt Holdings Limited | Financial services | 2023 |
| Akusika Business Services Limited | Professional & scientific | 2009 |
| Alex & Sons Building Landscaping and Gardening Services Ltd | Construction | 2025 |
| Allen Landscaping Ltd | Administrative & support | 2011 |
| AMA Plumbing & Heating Ltd | Construction | 2023 |
| Amazing Therapy Centre C.I.C. | Health & social care | 2023 |
| AND Jump Ltd | Professional & scientific | 2017 |
| Anglo Amersham Management Ltd | Real estate | 2005 |
| Anne Bruggink Consulting Ltd | Professional & scientific | 2016 |
| AON GAS Services Limited | Energy | 2009 |
| Aquaservice Water Engineering UK Limited | Other services | 2025 |
| AR Automotive Limited | Wholesale & retail | 2017 |
| Arcc Trading Ltd | Hospitality | 2025 |
| Archibuild London Ltd | Construction | 2015 |
| Archivision London Limited | Manufacturing | 2017 |
| ARK One Group Ltd | Construction | 2018 |
| Asif Associates Limited | Professional & scientific | 2021 |
| ASK More Coaching Ltd | Health & social care | 2024 |
| Aston House (Chinnor) Management Ltd | Extraterritorial | 2013 |
| AT Vehicle Maintenance Ltd | Wholesale & retail | 2024 |
| Atypical Property Projects Ltd | Real estate | 2024 |
| Auravia Limited | Health & social care | 2025 |
| Autogenius Limited | Wholesale & retail | 2020 |
| Aztec Electrical Ltd | Construction | 2018 |
| Bains Property Management Limited | Real estate | 2011 |
| BAT Accounting Services Ltd | Professional & scientific | 2010 |
| Baybright Limited | Hospitality | 1986 |
| Beaconsfield Roofing Ltd | Construction | 2021 |
| Belmont Management Consultants Limited | Professional & scientific | 1997 |
| Bennetts Yard (KB) Management Limited | Households | 2004 |
| Bernwode Homes Ltd | Construction | 2015 |
| Bespoke M Limited | Administrative & support | 2016 |
| Bespoke Masonry Ltd | Manufacturing | 2018 |
| Bhooee Engineering Ltd | Professional & scientific | 2025 |
| Bibz Limited | Administrative & support | 2021 |
| Bijou Living Limited | Construction | 2019 |
| Bison Pictures Ltd | Information & communication | 2022 |
| Bitpusher Limited | Information & communication | 2006 |
| Blandings Consulting Ltd | Professional & scientific | 2021 |
| Bohemian Sparks Group Limited | Information & communication | 2024 |
| Bonners & Babingtons Limited | Real estate | 2010 |
| Bonners and Babingtons (Lettings) Limited | Real estate | 2022 |
| Bonners and Babingtons (Princes Risborough) Limited | Real estate | 2020 |
| Bonners and Babingtons (Properties) Limited | Real estate | 2008 |
| Boostr Music LLP | | 2025 |
| Bowyer Properties LLP | | 2017 |
| Box Tree Creative Ltd | Arts & recreation | 2019 |
| Bradley Heating and Plumbing Ltd | Construction | 2023 |
| Bravo AI Limited | Information & communication | 2025 |
| Brickspeed Garage Ltd | Wholesale & retail | 2025 |
| Bright Sky Enterprises Ltd | Information & communication | 2018 |
| Brinetech Limited | Mining & quarrying | 2025 |
| Brook Homes Ltd | Construction | 1995 |
| Brownswood Consultancy Ltd | Professional & scientific | 2020 |
| Btcs Ltd | Information & communication | 2023 |
| Bucks Garden Care Ltd | Administrative & support | 2022 |
| Buds That Blossom Aylesbury Limited | Health & social care | 2022 |
| Buds That Blossom Chinnor Limited | Health & social care | 2021 |
| Buds That Blossom Limited | Health & social care | 2019 |
| Business Maps Limited | Professional & scientific | 1996 |
| Buttercup Apparel Limited | Wholesale & retail | 2021 |
| BW Building Group Ltd | Manufacturing | 2022 |
| C P Sparks Limited | Information & communication | 2016 |
| C&Bw Services LLP | | 2021 |
| C2 Human Factors Education CIC | Education | 2025 |
| C2 Human Factors Ltd | Education | 2025 |
| Caliber Financial Planning Limited | Financial services | 2009 |
| Candid Money Limited | Information & communication | 2009 |
| Carpenters Empire Ltd | Wholesale & retail | 2024 |
| Cater & Company Limited | Professional & scientific | 2008 |
| Cerynia Limited | Arts & recreation | 2021 |