| Balobasha Limited | Hospitality | 2026 |
| Balteram Limited | Real estate | 1999 |
| BAM Motorsport Management Limited | Administrative & support | 2020 |
| BAM Motorsport Promotions Limited | Other services | 2012 |
| BAM Motorsports Limited | Arts & recreation | 2009 |
| Bamber Lister Properties Limited | Real estate | 2025 |
| Bambi Kids Limited | Wholesale & retail | 2025 |
| Bambubar Ltd | Hospitality | 2021 |
| Band Factory Ltd | Arts & recreation | 2011 |
| Banner Jones Limited | Professional & scientific | 2008 |
| Bannister Publications Limited | Information & communication | 1991 |
| Banwood Properties Ltd | Real estate | 2024 |
| BAR21 (Chesterfield) Ltd | Hospitality | 2021 |
| Barber G Ltd | Other services | 2013 |
| Barbermazeme Ltd | Other services | 2020 |
| Barclays Web Crafters Ltd | Other services | 2025 |
| Barkers Plumbing and Heating Services Ltd | Construction | 2024 |
| Barktropolis Limited | Agriculture & fishing | 2024 |
| Barlborough Pharma Limited | Wholesale & retail | 2018 |
| Barlborough School House Trust | Arts & recreation | 2000 |
| Barlboroughs on Going Options Throughout Schooling Limited | Education | 2010 |
| Barley Homes Ltd | Construction | 2015 |
| Barley Mews Cottages Limited | Real estate | 1968 |
| Barlow Country Club Limited | Hospitality | 2022 |
| Barlow Fisheries Limited | Agriculture & fishing | 2019 |
| Barlow Rhodes Limited | Construction | 2024 |
| Barmy Army Limited | Other services | 1995 |
| Barn OWL Furniture Ltd | Manufacturing | 2024 |
| Barn to Box Ltd | Real estate | 2025 |
| Barnard Engineering Services Ltd | Administrative & support | 2012 |
| Barncroft Development Limited | Real estate | 2025 |
| Barnes Estates Limited | Hospitality | 2015 |
| Barnes Land & Property Limited | Real estate | 2017 |
| Barnes Woodlands Limited | Real estate | 2023 |
| Barre & Yoga Studio Ltd | Other services | 2022 |
| Barrons of Chesterfield Ltd | Wholesale & retail | 2010 |
| Barrow Hill Memorial Hall Ltd | Hospitality | 2025 |
| Barry Gollop Consulting Ltd | Information & communication | 2014 |
| Barsan Estates Ltd | Real estate | 2021 |
| Barthorpe Groundworks Ltd | Construction | 2015 |
| Barton Hill Croft Management Limited | Households | 2021 |
| Barton's Electrical Services Limited | Wholesale & retail | 2013 |
| Base Fitness Ltd | Wholesale & retail | 2022 |
| Basilisk Data Limited | Information & communication | 1994 |
| Bass Bags Limited | Wholesale & retail | 2013 |
| Batemans Investments Company Limited | Real estate | 2001 |
| Bates (Central) Limited | Real estate | 1964 |
| Bathra Ltd | Agriculture & fishing | 2020 |
| Baxter's Groom Shack Limited | Other services | 2024 |
| Bay Tree Accounting Limited | Professional & scientific | 2024 |
| Bayiravi Ltd | Agriculture & fishing | 2020 |
| Baysal Barber Ltd | Other services | 2024 |
| Baysallar Pizza Ltd | Hospitality | 2024 |
| BB Eight Seven Ltd | Professional & scientific | 2024 |
| BB Flexcare Solutions Limited | Health & social care | 2026 |
| BB Physiques Ltd | Arts & recreation | 2025 |
| BC Chesterfield Ltd | Health & social care | 2019 |
| BC Roofing Ltd | Construction | 2016 |
| BCB Amber Valley Ltd | Hospitality | 2018 |
| BCG Boxx Ltd | Education | 2024 |
| BCS Clinical Consulting Limited | Hospitality | 2011 |
| BE Bare Clinic Ltd | Other services | 2023 |
| BE Free to Grieve Ltd | Other services | 2023 |
| BE Inspired Media & Education Limited | Transport & logistics | 2018 |
| Beachcroft Solutions Limited | Professional & scientific | 2021 |
| Beaconmedaes Ltd | Manufacturing | 1994 |
| Bear Soles Limited | Education | 2009 |
| Bearwood Construction Ltd | Construction | 2019 |
| Beat Factory Records Limited | Arts & recreation | 2021 |
| Beatbridge Solutions Ltd | Information & communication | 2026 |
| Beauchief Estates (UK) Limited | Real estate | 2014 |
| Beaumont Care Contracts Limited | Health & social care | 2022 |
| Beaumont Care Homes Holdings Limited | Financial services | 2021 |
| Beaumont Care Homes Limited | Health & social care | 2021 |
| Beaumont Care Homes Property Limited | Real estate | 2021 |
| Beautique Beauty Ltd | Other services | 2014 |
| Beauty at Belle VIE Limited | Other services | 2018 |
| Beauty Care Academy and Aesthetics Limited | Education | 2025 |
| Beauty Growth Academy Chesterfield Limited | Professional & scientific | 2023 |
| Beauty Secrets by Laura Ltd | Education | 2026 |
| Beaver Contracts Limited | Construction | 2003 |
| Beck JJC Ltd | Health & social care | 2024 |
| Beck Procure Ltd | Other services | 2014 |
| Becker Orthopedic UK Limited | Wholesale & retail | 2005 |
| BEDIFFERENT2 Ltd | Wholesale & retail | 2024 |
| BEE Well Products Ltd | Manufacturing | 1994 |
| Beech Lodge Limited | Health & social care | 1994 |
| Beechers Brook Limited | Hospitality | 2012 |
| Beef King Chesterfield Limited | Hospitality | 2023 |
| Beehive Storage Limited | Real estate | 2012 |
| Beeley Close Management Company Ltd | Households | 2025 |
| Beeley Ltd | Professional & scientific | 2019 |
| Beepart Limited | Real estate | 1921 |
| Beeston Estates Limited | Construction | 2025 |
| Behavioural Therapy Ltd | Other services | 2020 |
| Behind the Business Lens Ltd | Professional & scientific | 2025 |
| Belcie Property Limited | Real estate | 2025 |
| Belcie Property Rentals Limited | Real estate | 2025 |
| Belfry Access Ltd | Construction | 2024 |
| Bella Mahes Pizzeria Ltd | Hospitality | 2024 |