| THE Spray Doctor Limited | Wholesale & retail | 2009 |
| THE Spread Eagle (Chesterfield) Ltd | Hospitality | 2019 |
| THE Storage Place Ltd | Transport & logistics | 2024 |
| THE Storeage Company Limited | Real estate | 2026 |
| THE Straight Escape Ltd | Wholesale & retail | 2025 |
| THE Sustainable Spirit Co. Ltd. | Manufacturing | 2006 |
| THE Tabletop Taphouse Ltd | Wholesale & retail | 2023 |
| THE Tall GUY Ltd | Hospitality | 2013 |
| THE Tanning Rooms Chesterfield Ltd | Other services | 2025 |
| THE Tile Studio by Lesley Webster Ltd | Wholesale & retail | 2017 |
| THE Trades Hub Limited | Professional & scientific | 2012 |
| THE Travelling VET Ltd | Professional & scientific | 2016 |
| THE UK Group (Chesterfield) Ltd | Construction | 2024 |
| THE Unique Gift Store Ltd | Wholesale & retail | 2013 |
| THE V.M. Specialist Limited | Professional & scientific | 2017 |
| THE Value Book-Keeping Ltd | Professional & scientific | 2020 |
| THE Van Yard Limited | Wholesale & retail | 2006 |
| THE Very Good PUB Company Ltd | Hospitality | 2014 |
| THE Very Important Marketing Corporation Ltd | Professional & scientific | 2023 |
| THE Village (Chesterfield) Limited | Administrative & support | 2015 |
| THE Vintage Hanger Limited | Wholesale & retail | 2022 |
| THE Volunteer Centre - Chesterfield and North East Derbyshire | Health & social care | 2005 |
| THE Watch Hub Ltd | Wholesale & retail | 2017 |
| THE Web & App Design Company Ltd | Wholesale & retail | 2026 |
| THE Wellness Brand CIC | Wholesale & retail | 2024 |
| THE Western Shop Ltd | Wholesale & retail | 2014 |
| THE Wholistic Coach Limited | Administrative & support | 2023 |
| THE Wilkinson VA Ltd | Administrative & support | 2023 |
| THE Window People Ltd | Construction | 2012 |
| THE Window Repair GUY Ltd | Construction | 2018 |
| THE Wood Fired Dough Co Limited | Wholesale & retail | 2020 |
| THE Workshop - Independent Specialist Limited | Wholesale & retail | 2025 |
| THE Workwear Shed Limited | Wholesale & retail | 2023 |
| Thea Property Group Ltd | Construction | 2023 |
| Them UP North Limited | Professional & scientific | 2019 |
| Themotorspecialists Ltd | Wholesale & retail | 2019 |
| Theotokos Services Limited | Administrative & support | 2025 |
| Therapist Limited | Other services | 2012 |
| Theserviceteam Ltd | Information & communication | 2015 |
| Thesius Limited | Public administration | 2017 |
| Theupsteam Ltd | Professional & scientific | 2023 |
| Thewellnesscompany Limited | Wholesale & retail | 2023 |
| Thillainathan Sritharan Limited | Construction | 2020 |
| Thirdway Strategies Ltd | Professional & scientific | 2025 |
| This IS Asha Ltd | Education | 2025 |
| Thistle Build Ltd | Construction | 2025 |
| Thistle Healthcare Services Limited | Health & social care | 2016 |
| Thomas Nathan Thacker Ltd | Extraterritorial | 2025 |
| Thomfam Holdings Limited | Financial services | 2026 |
| Thompson Cockers Ltd | Agriculture & fishing | 2023 |
| Thompson Podiatry & Foot Care Ltd | Health & social care | 2020 |
| Thomson’S Auto Locksmiths Ltd | Wholesale & retail | 2025 |
| Thor-Weld Industries Limited | Extraterritorial | 1988 |
| Thorley International Transport Services Limited | Transport & logistics | 2013 |
| Thornit Ltd | Wholesale & retail | 2005 |
| Thorworld Industries Limited | Manufacturing | 1986 |
| THQ Chesterfield Ltd | Arts & recreation | 2023 |
| Three Counties Flying School C.I.C. | Education | 2023 |
| Three Pillar Trading Ltd | Information & communication | 2025 |
| Three S Property Limited | Extraterritorial | 2016 |
| Thrux Ltd | Administrative & support | 2025 |
| Thsh Consulting Ltd | Professional & scientific | 2025 |
| Tiam Food Ltd | Hospitality | 2025 |
| Ticklemybeard Ltd | Other services | 2026 |
| Tiddly Drinks Ltd | Hospitality | 2024 |
| Tigerprecision Ltd | Manufacturing | 2023 |
| Tiley Medica CIC | Health & social care | 2025 |
| Tilly Tinker Ltd | Manufacturing | 2020 |
| TIM Shepherd Properties Limited | Real estate | 2025 |
| TIM Wilton Site Services Ltd | Construction | 2016 |
| Timbercraft Design Limited | Manufacturing | 2009 |
| Timberline Joinery Services Limited | Construction | 2025 |
| Time Fire Solutions Ltd | Construction | 2025 |
| Time to BE Ltd | Extraterritorial | 2011 |
| Timepro Limited | Information & communication | 2004 |
| Timm Holdings Limited | Financial services | 2022 |
| Tinkley Lane Bidco Ltd | Financial services | 2023 |
| Tiny Tags Ltd | Wholesale & retail | 2023 |
| Tiny Tots Childcare Limited | Health & social care | 2015 |
| Tipi & Kata Limited | Arts & recreation | 2012 |
| Tishon Retail Ltd | Wholesale & retail | 2024 |
| TIT Chicken Ltd | Hospitality | 2024 |
| Titan Trackhire Limited | Other services | 2022 |
| Tj&H Denestates Limited | Real estate | 2026 |
| Tj&H Denservices Allestree Limited | Health & social care | 2026 |
| Tj&H Denservices Limited | Health & social care | 2026 |
| TJA Investments Limited | Real estate | 2012 |
| TJA Properties Limited | Real estate | 2013 |
| TJD Consultancy & Training Limited | Administrative & support | 2016 |
| TJD Engineering Solutions Limited | Information & communication | 2016 |
| TJG Brickworks Ltd | Construction | 2018 |
| TJI Property Limited | Real estate | 2026 |
| TJK Driving Limited | Transport & logistics | 2023 |
| TJP Telecomms Limited | Professional & scientific | 2015 |
| TLC Care Bidco Limited | Financial services | 2018 |
| TLC Care Homes Blamsters Residential Limited | Health & social care | 2019 |
| TLC Care Homes Clacton Residential Limited | Health & social care | 2019 |
| TLC Care Homes Colchester Supported Living Limited | Health & social care | 2019 |
| TLC Care Homes Limited | Health & social care | 1996 |
| TLC Care Homes Supported Living Limited | Health & social care | 2019 |