| ASB Consulting UK Ltd | Professional & scientific | 2024 |
| Asbestos Control Ltd | Construction | 2024 |
| ASG Seals Ltd | Wholesale & retail | 2014 |
| Asha Installations Limited | Construction | 2006 |
| Ashburton Housing Limited | Households | 2025 |
| Ashcroft SC Ltd | Health & social care | 2023 |
| Ashdell Finance Limited | Financial services | 2025 |
| Ashe Group (UK) Limited | Health & social care | 2010 |
| Ashese Visionary Limited | Other services | 2014 |
| Ashgate Electrical Ltd | Construction | 2012 |
| Ashgate Heritage Arts Community Interest Company | Arts & recreation | 2019 |
| Ashgate Hospicecare | Health & social care | 1988 |
| Ashgate Hospicecare Shops Limited | Wholesale & retail | 1991 |
| Ashington Blasting Services Limited | Construction | 2026 |
| Ashlea Trading Co. Ltd. | Wholesale & retail | 1996 |
| Ashley Thomas Limited | Professional & scientific | 2013 |
| Ashoka Cuisine Ltd | Hospitality | 2018 |
| Ashover Agricultural & Horticultural Society | Other services | 1998 |
| Ashover Brew Co Bars Ltd | Hospitality | 2026 |
| Ashover Brew Co Ltd | Manufacturing | 2025 |
| Ashover Brewery Limited | Manufacturing | 2012 |
| Ashover Community Medical Centre Ltd | Real estate | 2013 |
| Ashover Construction Ltd | Construction | 2026 |
| Ashover Landscaping Ltd | Administrative & support | 2023 |
| Ashover Limited | Professional & scientific | 2013 |
| Ashthorne Limited | Construction | 2015 |
| Ashton Mechanical & Electrical Limited | Construction | 2025 |
| Ashton Plumbing & Heating Limited | Construction | 2005 |
| ASJ Drummond Limited | Wholesale & retail | 2007 |
| ASK4 Logistics Group Ltd | Wholesale & retail | 2026 |
| Aspinall Crabb & Daughters Ltd | Real estate | 2021 |
| Aspirant Construction Ltd | Construction | 2006 |
| Aspire Advocacy Limited | Health & social care | 2024 |
| Aspire Brickwork Limited | Construction | 2003 |
| Aspire Business Support Ltd | Administrative & support | 2011 |
| Aspire Eco Energy Limited | Construction | 2010 |
| Aspire Games Ltd | Wholesale & retail | 2026 |
| Aspire Gates Limited | Manufacturing | 2003 |
| Aspire Pediatrics Ltd | Health & social care | 2026 |
| Aspire Property (Chesterfield) Limited | Real estate | 2024 |
| Aspire Safety and Health Limited | Professional & scientific | 2018 |
| Aspirion Wealth Limited | Financial services | 2016 |
| Assembledwears Ltd | Wholesale & retail | 2024 |
| Asset Complete Property Solutions Limited | Construction | 2007 |
| Asset Drop Limited | Wholesale & retail | 2017 |
| Asset Maintenance (Chesterfield) Limited | Construction | 2022 |
| Assure Education Limited | Administrative & support | 2014 |
| Assure Secure Protect Ltd | Financial services | 2012 |
| Assured Property Ltd | Real estate | 2017 |
| Astari Communications UK Limited | Information & communication | 2024 |
| Astley Bros. Ltd | Construction | 2016 |
| Aston Manor Construction Ltd | Construction | 2019 |
| Astor Construction Limited | Construction | 2014 |
| Astra Designs UK Ltd | Wholesale & retail | 2021 |
| Astrada Property Management Ltd | Households | 2008 |
| Astralis Arena Ltd | Education | 2025 |
| Astralis Intelligence Ltd | Information & communication | 2025 |
| Astro Branding Services Ltd | Professional & scientific | 2022 |
| Astute Executive Recruitment Limited | Administrative & support | 2016 |
| Astute Financial Recruitment Limited | Administrative & support | 2015 |
| ATB Civil Engineering Limited | Construction | 2012 |
| Athena Global Ltd | Information & communication | 2024 |
| Athena Moon Ltd | Real estate | 2023 |
| Atkinson Construction Group Ltd | Professional & scientific | 1991 |
| Atkinson Electrical Installations Ltd | Construction | 2014 |
| Atkinson F Holdings Ltd | Financial services | 2025 |
| Atkinson Homes (Hickleton) Ltd | Construction | 2016 |
| Atlantean Graphics Limited | Manufacturing | 2024 |
| Atlantic Pumps Group Limited | Financial services | 2024 |
| Atlantic Pumps Limited | Manufacturing | 2015 |
| Atlas Production Studio Ltd | Information & communication | 2019 |
| Atob Football Ltd | Arts & recreation | 2024 |
| Atollie Property Management Ltd. | Real estate | 2011 |
| Atoz Property Invest Ltd | Real estate | 2021 |
| Attest Digital Ltd | Information & communication | 2020 |
| Audioscene Limited | Real estate | 2002 |
| Aumann Housing Limited | Real estate | 2023 |
| Aurora FM Limited | Manufacturing | 2022 |
| Aurora Homes (Derbyshire) Ltd | Construction | 2024 |
| Ausom Vehicle Management Limited | Transport & logistics | 2007 |
| Austin Fletcher Limited | Professional & scientific | 2019 |
| Austins Electrical Ltd | Construction | 2007 |
| Auto Ease Ltd | Wholesale & retail | 2025 |
| Auto Reform Ltd | Wholesale & retail | 2026 |
| Auto Testing Centre Limited | Wholesale & retail | 2006 |
| Auto-Bright Valeting Ltd | Wholesale & retail | 2019 |
| AUTO2018 Car Sales Ltd | Wholesale & retail | 2022 |
| Autochx Limited | Financial services | 1999 |
| Autoclean Valeting Ltd | Administrative & support | 2025 |
| Autocraft MC Staveley Limited | Wholesale & retail | 2017 |
| Autofix 2018 Limited | Wholesale & retail | 2018 |
| Autofixblundell Ltd | Wholesale & retail | 2025 |
| Autoinspire (Chesterfield) Ltd | Wholesale & retail | 2018 |
| Autologix Limited | Wholesale & retail | 2006 |
| Automotive Compliance Limited | Information & communication | 2008 |
| Automotive Distributors Limited | Wholesale & retail | 2014 |
| Automotive Solutions Chesterfield Limited | Wholesale & retail | 2013 |
| Autoscreens Direct Limited | Wholesale & retail | 2001 |
| Autoshine DP Ltd | Wholesale & retail | 2025 |
| Autosol (UK) Limited | Wholesale & retail | 1989 |