| E J Medical Limited | Health & social care | 2025 |
| E J Redford Property Ltd | Real estate | 2024 |
| E Jarvis Lettings Limited | Real estate | 2024 |
| E Maculate Mobile Valeting Ltd | Wholesale & retail | 2019 |
| E N Printing Ltd | Manufacturing | 2023 |
| E Solutions Ltd | Administrative & support | 2014 |
| E T Power Systems Limited | Wholesale & retail | 1998 |
| E&S Beauty Ltd | Other services | 2023 |
| E&S Derbyshire Properties Ltd | Real estate | 2026 |
| E-Preparation Ltd | Information & communication | 2018 |
| E-Rock Care Services Ltd | Health & social care | 2021 |
| E. Fletcher (Chesterfield) Limited | Wholesale & retail | 1974 |
| EAB Property Services Limited | Real estate | 2024 |
| Eade Construction Group Ltd | Construction | 2025 |
| Eades Holdings Limited | Health & social care | 2020 |
| Eagle Building Specialists Limited | Construction | 2016 |
| Eagle Green Installations Limited | Wholesale & retail | 2019 |
| Eagle View Consulting Limited | Health & social care | 2021 |
| Earlystate Limited | Manufacturing | 2001 |
| Earthbeat Energy Ltd | Construction | 2019 |
| Eason YU Limited | Hospitality | 2019 |
| East Lincolnshire Emergency Vets Ltd | Professional & scientific | 2021 |
| East Midlands Asset Funding Limited | Financial services | 2014 |
| East Midlands Business Crime Forum | Administrative & support | 2010 |
| East Midlands Chamber (Derbyshire, Nottinghamshire, Leicestershire) | Other services | 1984 |
| East Midlands Chambers of Commerce International LLP | | 2011 |
| East Midlands Investors Limited | Extraterritorial | 2009 |
| East Midlands Strategic Land Ltd | Construction | 2016 |
| East Midlands Thermal Insulations Limited | Wholesale & retail | 2006 |
| Eastbank House Residents Limited | Real estate | 2013 |
| Eastcliffe News Shops Limited | Wholesale & retail | 1996 |
| Eastlands Management Ltd | Real estate | 2000 |
| Easton Properties Limited | Water & waste | 1996 |
| Easy Action Limited | Wholesale & retail | 2008 |
| Easyliving Landscaping Limited | Administrative & support | 2021 |
| Easyramp Limited | Extraterritorial | 2007 |
| Easyramps Limited | Extraterritorial | 2008 |
| EAT & Go Ltd | Hospitality | 2022 |
| EB Aesthetics Group Ltd | Wholesale & retail | 2023 |
| Ebenezer Logistics and Service Ltd | Transport & logistics | 2025 |
| Ebero Pipe Systems Limited | Wholesale & retail | 2006 |
| EBG Driver Ltd | Transport & logistics | 2025 |
| Ebonyh Care Ltd | Public administration | 2022 |
| Ebury Court Residential Home Limited | Health & social care | 2003 |
| Ebusiness Works Limited | Information & communication | 2013 |
| Echoo Core UK Ltd | Information & communication | 2025 |
| Eckington Land Limited | Real estate | 2001 |
| Eco Ampz Ltd Ltd | Manufacturing | 2022 |
| Eco HAB Limited | Construction | 2009 |
| Eco Hybrid Cars Limited | Wholesale & retail | 2025 |
| Eco Plastic Group Ltd | Manufacturing | 2025 |
| Eco Print Euro Ltd | Manufacturing | 2013 |
| Eco Process Tech Limited | Administrative & support | 2022 |
| Ecoclean Commercial Limited | Administrative & support | 2024 |
| Ecoexecutive Travel Ltd | Transport & logistics | 2016 |
| Ecotech Engineers Limited | Professional & scientific | 2020 |
| Ecsiteservices Ltd | Water & waste | 2025 |
| Eden Electronics Limited | Professional & scientific | 2005 |
| Eden Global Limited | Hospitality | 2024 |
| Eden GM Ltd | Administrative & support | 2021 |
| Eden Properties Chesterfield Limited | Real estate | 2013 |
| Eden Source Limited | Health & social care | 2019 |
| Edenberry Group Ltd | Health & social care | 2025 |
| Edifice Assets Ltd | Construction | 2026 |
| Edmund Street Activity Centre Ltd | Health & social care | 2019 |
| Education in First Gear Ltd | Education | 2026 |
| Educational Computing Solutions Ltd | Education | 2020 |
| Educational Psychology Consultation Assessment & Support (E.P.C.A.S) Ltd | Education | 2016 |
| Edward Serrell Plumbing & Heating Ltd | Construction | 2012 |
| Edwards Joinery and Building Contractors Limited | Construction | 2009 |
| Edwards Logistic Solutions Ltd | Transport & logistics | 2019 |
| Edwards of Derbyshire Ltd | Agriculture & fishing | 2025 |
| Edwin Bikes Limited | Manufacturing | 2021 |
| Eggbod Information Technology Services Limited | Information & communication | 2010 |
| Egod Investment Limited | Health & social care | 2020 |
| EHJ Limited | Wholesale & retail | 2025 |
| Eigengrau Productions Ltd | Information & communication | 2024 |
| Eirflow Ltd | Energy | 2026 |
| EJB Homes Ltd | Real estate | 2022 |
| EJG Solutions Ltd | Professional & scientific | 2023 |
| Ejmc Ltd | Manufacturing | 2019 |
| Ekraj Limited | Real estate | 2005 |
| Elangasinghe Limited | Health & social care | 2013 |
| Elastic FM Limited | Information & communication | 2011 |
| Elba Logistics Limited | Transport & logistics | 2021 |
| Elder Bars Ltd | Hospitality | 2024 |
| Elec Recruitment Ltd | Administrative & support | 2024 |
| Electraplas Limited | Manufacturing | 1983 |
| Electric Eales Limited | Construction | 2022 |
| Electricon (Chesterfield) Ltd | Construction | 2023 |
| Electrified Marketing Limited | Extraterritorial | 2016 |
| Electrophysical Technologies Ltd | Extraterritorial | 2021 |
| Electrosteel Castings (UK) Limited | Manufacturing | 2000 |
| Electrotest Marine Ltd | Transport & logistics | 2019 |
| Element Mechanical Services Limited | Construction | 2015 |
| Elemental Academy Ltd | Arts & recreation | 2025 |
| Elendra Ltd | Wholesale & retail | 2025 |
| Elephants in Rooms Ltd | Education | 2022 |
| Elete Ltd | Public administration | 2026 |
| Elevate Aesthetics Ltd | Other services | 2026 |