| A D Tyres (Wholesale) Limited | Wholesale & retail | 2000 |
| A F P Properties Limited | Real estate | 2025 |
| A G GAS & Plumbing Services Ltd | Construction | 2025 |
| A Hardy Electrical Limited | Construction | 2025 |
| A J Clarke Consulting Ltd | Information & communication | 2025 |
| A J Graves Limited | Professional & scientific | 2017 |
| A J Hill Holdings Limited | Professional & scientific | 2021 |
| A J Properties (Chesterfield) Limited | Real estate | 2023 |
| A J Ward Transport (Chesterfield) Ltd | Transport & logistics | 2024 |
| A K 2005 Limited | Administrative & support | 2013 |
| A K Computer Systems & Consulting Limited | Information & communication | 1998 |
| A K Harmony Limited | Wholesale & retail | 2022 |
| A L Metal Fabrications Limited | Manufacturing | 2021 |
| A LA Carte Business Services Limited | Transport & logistics | 2002 |
| A Line (Chesterfield) Ltd | Transport & logistics | 2011 |
| A Line Travel Limited | Administrative & support | 2020 |
| A Marples Landscapes Limited | Construction | 2017 |
| A Morton Financial Planning Ltd | Professional & scientific | 2020 |
| A O Storage Limited | Transport & logistics | 2018 |
| A P Building Design (Commercial) Limited | Professional & scientific | 2015 |
| A P Building Design Limited | Professional & scientific | 2013 |
| A P Henman Ltd | Manufacturing | 2025 |
| A P Morris Limited | Construction | 2020 |
| A R Transport Solutions Limited | Transport & logistics | 2009 |
| A S I Security Systems Limited | Administrative & support | 2010 |
| A Star Leisure Ltd | Arts & recreation | 2006 |
| A T Commercial Finance 2 Limited | Financial services | 2023 |
| A T Commercial Finance Limited | Financial services | 2021 |
| A T D Precision Engineering Ltd | Manufacturing | 2006 |
| A Team Cranes UK Ltd | Manufacturing | 2022 |
| A to Z Business Solutions Limited | Administrative & support | 2019 |
| A UP Marketing Ltd | Professional & scientific | 2010 |
| A W Structures Limited | Professional & scientific | 2019 |
| A&A Carwash Detailing Ltd | Administrative & support | 2026 |
| A&D Groundworks Chesterfield Ltd | Construction | 2017 |
| A&J Richardson Holdings Limited | Financial services | 2026 |
| A&V Distribution Limited | Wholesale & retail | 2021 |
| A-L Mechanical Engineering Services Limited | Professional & scientific | 2022 |
| A-Rock Construction Ltd. | Construction | 2011 |
| A-Rock Limited | Real estate | 2006 |
| A. Count 'N' C Ltd | Financial services | 2009 |
| A. Pearson & Co. Limited | Agriculture & fishing | 1954 |
| A.C.I Solutions Ltd | Wholesale & retail | 2024 |
| A.I.M Flooring Solutions Ltd | Construction | 2023 |
| A.J.Harris and Son(Farmers)Limited | Agriculture & fishing | 1952 |
| A.Jones Engineering Ltd | Professional & scientific | 2024 |
| A.L.Gladwin Ltd | Hospitality | 2019 |
| A.Leary Tiling Ltd | Construction | 2018 |
| A.P.T. Patterns Limited | Manufacturing | 2005 |
| A1 Auto Locksmiths Limited | Wholesale & retail | 2025 |
| A1 Engineering Surplus Limited | Wholesale & retail | 2022 |
| A1 Garage Doors (UK) Ltd | Construction | 2015 |
| A1 Nails & Tanning Ltd | Other services | 2011 |
| A61 Autos and Recovery Ltd | Wholesale & retail | 2023 |
| A61 Bathrooms Limited | Wholesale & retail | 2016 |
| Aaem Limited | Construction | 2025 |
| AAG Cyber Security Limited | Extraterritorial | 2002 |
| AAG I.T. Services Ltd | Information & communication | 2013 |
| Aaordidge Ltd | Other services | 2022 |
| Aaron Industries Ltd | Hospitality | 2025 |
| Aaron Michael Humphries Limited | Real estate | 2025 |
| Aaroncare Limited | Health & social care | 1986 |
| Aasdz Limited | Real estate | 2015 |
| AAV Car Parts Ltd | Wholesale & retail | 2023 |
| AAV Properties Ltd | Real estate | 2025 |
| AB Civils Design Ltd | Administrative & support | 2018 |
| AB Detailing Ltd | Wholesale & retail | 2024 |
| AB Plastering & Rendering Ltd | Construction | 2026 |
| AB Race Cruisers Limited | Manufacturing | 2017 |
| ABA Mechanical Limited | Construction | 2016 |
| Abacus Funding Ltd | Financial services | 2014 |
| Abatech Ltd | Information & communication | 2018 |
| Abbey Hill Complete Electrical Ltd | Construction | 2016 |
| Abbey Perfumery Limited | Manufacturing | 2023 |
| Abbey Portable Buildings Limited | | 1961 |
| Abbotsbury Contractors Limited | Construction | 2003 |
| ABC (Chesterfield) Ltd | Wholesale & retail | 2025 |
| ABC Fire Solutions Limited | Public administration | 2009 |
| ABC Nursery School Limited | Education | 2011 |
| Abca Holdings Ltd | Construction | 2026 |
| Abcode Ltd | Information & communication | 2024 |
| Abel Systems Limited | Manufacturing | 1985 |
| Abella Beauty Studio Limited | Other services | 2025 |
| Abesther Care Cohesion Limited | Administrative & support | 2026 |
| ABH (Holdings) Ltd | Financial services | 2023 |
| ABI Financial Services Ltd | Financial services | 2025 |
| Abik Food Ltd | Hospitality | 2025 |
| Ability Supported Living Derbyshire Limited | Hospitality | 2013 |
| Abisso International Ltd | Real estate | 2025 |
| Abiton Limited | Wholesale & retail | 2002 |
| Able Alterations and Improvements Ltd | Construction | 2026 |
| ABM Radiology Limited | Health & social care | 2025 |
| ABR Construction (Cobnash) Limited | Construction | 2025 |
| Abrahams Equestrian Ltd | Agriculture & fishing | 2019 |
| AC Events Crewing Ltd | Arts & recreation | 2019 |
| AC Plant Services Ltd | Wholesale & retail | 2021 |
| Acacia Property Solutions Ltd. | Real estate | 2017 |
| Acacia Psychology Ltd | Health & social care | 2025 |
| Acaris AG Limited | Information & communication | 2026 |
| Access 4 U (Scaffolding) Ltd | Construction | 2009 |