| Elmnt KBN Limited | Wholesale & retail | 2025 |
| Elms Court RTM Company Ltd | Real estate | 2019 |
| Elobau UK Limited | Administrative & support | 2008 |
| Elorarez Limited | Financial services | 2022 |
| Elpoint Ltd | Administrative & support | 2011 |
| Elsayed Ahmed DOH Ltd | Health & social care | 2025 |
| Elson Services Limited | Transport & logistics | 2018 |
| Eltom Consulting Ltd | Wholesale & retail | 2011 |
| Elton JFC Ltd | Arts & recreation | 2025 |
| Elucidate Group Ltd | Professional & scientific | 2016 |
| Elworth Park Management Company Limited | Households | 2014 |
| Elysium Health and Fitness (Aldford) Ltd | Other services | 2016 |
| Elysium Wellness Limited | Health & social care | 2025 |
| Elysium-Chambers Ltd | Professional & scientific | 2013 |
| Emanuel Oliver Limited | Professional & scientific | 2015 |
| Embark Chemical Consulting Ltd | Professional & scientific | 2024 |
| Emberton Marketing Limited | Professional & scientific | 2020 |
| Embrace Education Ltd | Information & communication | 2020 |
| Emco Investments Ltd | Real estate | 2021 |
| Emdr Treatment Ltd | Health & social care | 2023 |
| Emello Limited | Wholesale & retail | 2025 |
| Emerald Insight IT Consultancy Limited | Information & communication | 2025 |
| Emerane Ltd | Information & communication | 2023 |
| Emerge Online Limited | Professional & scientific | 2018 |
| Emergency Department Staffing Limited | Administrative & support | 2014 |
| Emergency Healthcare Staffing Limited | Administrative & support | 2014 |
| Emergent Group Ltd | Manufacturing | 2017 |
| Emerson Bond Limited | Education | 2015 |
| Emery Developments Limited | Real estate | 1996 |
| Emery Farm Estates Limited | Real estate | 1961 |
| Emery Investments Limited | Real estate | 1956 |
| Emery Properties Limited | Real estate | 1996 |
| EMF Associates Limited | Professional & scientific | 2023 |
| EMJ Chester Property Limited | Real estate | 2025 |
| Emma Mackley Private Travel Limited | Administrative & support | 2022 |
| Emma Woolley Ltd | Wholesale & retail | 2017 |
| Emmanuel Environmental Ltd | Administrative & support | 2018 |
| Empartna Ltd | Professional & scientific | 2025 |
| Empire Fencing and Gates Ltd | Administrative & support | 2021 |
| Empire Global Finance Ltd | Financial services | 2013 |
| Employee Passport Limited | Information & communication | 2026 |
| Empor Magazine | Financial services | 2020 |
| Emporium Power Property Solutions Limited | Households | 2025 |
| Emree Limited | Financial services | 2016 |
| Emrol Estates Limited | Real estate | 2019 |
| EMU Estates Ltd | Real estate | 2023 |
| EN Powerclean Ltd | Administrative & support | 2026 |
| ENC Shop Management Co. | | 2008 |
| Enchanted Patisserie Ltd | Hospitality | 2022 |
| Endeavour Education Ltd | Education | 2024 |
| Endless Energy Limited | Construction | 2006 |
| Enduring Gardener Limited | Information & communication | 2014 |
| Enem Estate Ltd | Real estate | 2025 |
| Energy Generation Experts Ltd | Professional & scientific | 2024 |
| Energy Service Solutions Ltd | Energy | 2019 |
| Enernetiq Limited | Information & communication | 2011 |
| Engage Presenters Limited | Education | 2013 |
| Engine House Management Company Limited | Households | 2014 |
| Engineered Composites Holdings Limited | Financial services | 2021 |
| Engineered Composites Limited | Manufacturing | 1986 |
| Engineering Developments Limited | Manufacturing | 2006 |
| Enhance Industries Limited | Financial services | 2024 |
| Enlightened Money Ltd | Professional & scientific | 2012 |
| Enofaber Ltd | Wholesale & retail | 2021 |
| Enosis Properties Limited | Real estate | 2023 |
| Enrichment Technology Company Limited | Manufacturing | 2003 |
| Enrichment Technology UK Limited | Construction | 2003 |
| ENS Care and Support Limited | Health & social care | 1991 |
| Ensemble Performing Arts Education C.I.C. | Arts & recreation | 2025 |
| Entsurgerynet Ltd. | Health & social care | 2014 |
| Entyce Creative Limited | Professional & scientific | 2008 |
| Entyce Property Ltd | Real estate | 2015 |
| Envenergia Solutions Ltd | Professional & scientific | 2023 |
| Envirohale Limited | Professional & scientific | 2023 |
| Environelle Ltd | Other services | 2022 |
| ENW Limited | Manufacturing | 2005 |
| EOS Bespoke Ltd | Manufacturing | 2024 |
| EP Law Ltd | Professional & scientific | 2022 |
| EP Marine & Rail Ltd | Professional & scientific | 2022 |
| EP Pubs Ltd | Hospitality | 2020 |
| Ephesus Estates Limited | Real estate | 2020 |
| Ephesus Grill Ltd | Hospitality | 2019 |
| Epic Getaways Ltd | Administrative & support | 2025 |
| Epicbite Ltd | Hospitality | 2023 |
| Epicurio Limited | Wholesale & retail | 2009 |
| Epmsec Limited | Administrative & support | 2025 |
| Epona Ventures Limited | Hospitality | 2017 |
| Epoxy FOX Limited | Construction | 2022 |
| Equi-Spread Limited | Manufacturing | 2024 |
| Equicantis Ltd | Information & communication | 2022 |
| Equilibrium Movement Ltd | Arts & recreation | 2020 |
| Equimed Europe Ltd | Wholesale & retail | 2005 |
| Equinett Ltd | Wholesale & retail | 2024 |
| Equistables Direct Ltd | Manufacturing | 2021 |
| Eren Family Foods Ltd | Wholesale & retail | 2024 |
| Eric London Limited | Financial services | 2019 |
| ERJ Limited | Wholesale & retail | 2007 |
| Erlas Black Wood (Holdings) Limited | Financial services | 2019 |
| Erlas Black Wood Limited | Real estate | 2019 |
| Erlas Black Wood NO 2 Limited | Financial services | 2019 |