| Advanced Coated Products Limited | Manufacturing | 2015 |
| Advanced Skin Cheltenham Limited | Health & social care | 2021 |
| Advanced Skin Cheltenham Plasma Limited | Health & social care | 2021 |
| Advancedplan Limited | Professional & scientific | 1994 |
| Advantage Sports Management Limited | Arts & recreation | 2015 |
| Advent Broker Services Ltd | Financial services | 2022 |
| Advent Insurance Services Limited | Financial services | 1998 |
| Adventure Capital Partners Limited | Professional & scientific | 1997 |
| Adventure Riding Experience Ltd | Arts & recreation | 2019 |
| Adventureland Soft Play Ltd | Arts & recreation | 2025 |
| ADVS2000 Limited | Information & communication | 2010 |
| AE Cheltenham Ltd | Real estate | 2023 |
| Aegeus Properties,Limited | Real estate | 1957 |
| Aegles Health Limited | Health & social care | 2014 |
| Aemulator CIC | Real estate | 2021 |
| Aeneid Nominees Limited | Professional & scientific | 2013 |
| Aero Doctor Ltd. | Health & social care | 2017 |
| Aerobatx Ltd | Wholesale & retail | 2020 |
| Aerocad Design Services Limited | Manufacturing | 2006 |
| Aerodynamics DRY Leasing Ltd | Manufacturing | 1985 |
| Aeroforma Technologies (West) Limited | Manufacturing | 2003 |
| Aeroforma Technologies Group Limited | Administrative & support | 1997 |
| Aeroforma Technologies Holdings Limited | Financial services | 2020 |
| Aerospike UK Limited | Administrative & support | 2016 |
| Aerton Limited | Professional & scientific | 1988 |
| AES Terra Property Ltd | Real estate | 2026 |
| Aesthetic Prosthetic Limited | Education | 2021 |
| Aevum Consultancy Ltd | Information & communication | 2018 |
| AF Scaffolding Cotswolds Ltd | Construction | 2021 |
| AFC Finners Limited | Information & communication | 2022 |
| Afdos Pharmaceuticals Limited | Wholesale & retail | 2014 |
| Affable Explorations Limited | Hospitality | 2012 |
| Affluens Realty Ltd | Real estate | 2023 |
| Affric Consulting Ltd | Professional & scientific | 2020 |
| Afgf Limited | Arts & recreation | 2018 |
| Afgf Properties Ltd | Real estate | 2018 |
| Afkdlt Ltd | Professional & scientific | 2024 |
| AFKO2 Ltd | Administrative & support | 2024 |
| Afmjk Ltd | Wholesale & retail | 2015 |
| Afrasia Ltd | Arts & recreation | 2021 |
| Afreen Spice Limited | Hospitality | 2017 |
| Afrls Ltd | Administrative & support | 2024 |
| Afroscool Ltd | Wholesale & retail | 2025 |
| AFX Audio Visual Services Limited | Information & communication | 2010 |
| AG Agri Ltd | Agriculture & fishing | 2018 |
| AG Carpet Specialist Ltd | Administrative & support | 2025 |
| Agasco Biochar Limited | Extraterritorial | 2025 |
| Agasco Household Devices Africa Limited | Professional & scientific | 2022 |
| Agasco Limited | Other services | 2017 |
| Agate Holdings Limited | Information & communication | 2017 |
| AGC Pet Products Limited | Wholesale & retail | 2021 |
| AGD Systems Limited | Manufacturing | 1991 |
| Agency Associates Ltd | Real estate | 2020 |
| Aggregate Developments Limited | Real estate | 1995 |
| Aggs Hill Limited | Professional & scientific | 2001 |
| Aghoco 1256 Limited | Financial services | 2014 |
| Agilico (Holdings) Limited | Financial services | 2026 |
| Agilico Bidco Limited | Financial services | 2018 |
| Agilico Group Limited | Financial services | 2018 |
| Agilico Holdco Limited | Financial services | 2018 |
| Agilico Midco Limited | Financial services | 2018 |
| Agilico Software Limited | Information & communication | 1997 |
| Agilico Systems Limited | Manufacturing | 1990 |
| Agilico Workplace Technologies (Midlands) Limited | Wholesale & retail | 1969 |
| Agilico Workplace Technologies (North) Limited | Wholesale & retail | 1987 |
| Agilico Workplace Technology Limited | Wholesale & retail | 1990 |
| Agilis Recruitment Ltd | Administrative & support | 2016 |
| Agimude Limited | Real estate | 1977 |
| Agri Mush Limited | Agriculture & fishing | 2022 |
| Agrifinance Ltd | Financial services | 2013 |
| Agrimax Limited | Wholesale & retail | 1999 |
| Agrinergy Limited | Professional & scientific | 2003 |
| Agriselect (PVT) Limited | Agriculture & fishing | 2014 |
| Agriworx Cotswolds Ltd | Agriculture & fishing | 2023 |
| AH Care Ltd | Health & social care | 2011 |
| AH Cheltenham Ltd | Hospitality | 2019 |
| AHP Property Developments Limited | Construction | 2019 |
| AI Biomed Limited | Health & social care | 2023 |
| AI Studio 24/7 Ltd | Information & communication | 2026 |
| Aichlo Limited | Construction | 2015 |
| AIM UP | Health & social care | 2001 |
| Ainsly Limited | Wholesale & retail | 2017 |
| Air DM Ltd | Transport & logistics | 2022 |
| Air Scaffolding Solutions Ltd | Construction | 2025 |
| Air Sea Freight Limited | Extraterritorial | 2014 |
| Air Traffic Services Resourcing Solutions Ltd | Transport & logistics | 2019 |
| Aircraft Production Tooling Co. Limited | Construction | 1958 |
| Aircraft Tractor Parts Ltd | Wholesale & retail | 2020 |
| Airdeska Ltd | Other services | 2019 |
| Airline Investments Limited | Professional & scientific | 2012 |
| Airpeople Limited | Real estate | 2001 |
| Airportax Ltd | Transport & logistics | 2025 |
| Airtime Aerobatics Limited | Arts & recreation | 2006 |
| Airvault Ltd | Information & communication | 2022 |
| Aithra Limited | Professional & scientific | 2018 |
| Aixcll Ltd | Extraterritorial | 2026 |
| AJ Architects Limited | Professional & scientific | 2011 |
| AJ Consultancy (Cheltenham) Limited | Professional & scientific | 2025 |
| AJ Lockey Services Ltd | Construction | 2022 |
| AJ Process Engineering Ltd | Professional & scientific | 2015 |