| Fitzroy Lending Limited | Financial services | 2023 |
| Five Alls Management Ltd | Hospitality | 2019 |
| Five Financial Planning Group Ltd | Financial services | 2024 |
| Five Keys Family Office (UK) Limited | Professional & scientific | 2021 |
| Five Valleys Financial Planning Ltd | Professional & scientific | 2020 |
| Five Valleys HR Limited | Professional & scientific | 2020 |
| FIX Heating Ltd | Construction | 2025 |
| Fixaball Ltd. | Professional & scientific | 2021 |
| FJ Glacier Properties Ltd | Real estate | 2021 |
| FJS Property Group Ltd | Real estate | 2026 |
| Flag Vehicle Deliveries Limited | Administrative & support | 2010 |
| Flame 01 Ltd | Hospitality | 2025 |
| Flaming Chelt Ltd | Hospitality | 2023 |
| Flamingo Bay Ltd | Information & communication | 2023 |
| Flasko Limited | Professional & scientific | 2018 |
| Flawless Landscapes Limited | Administrative & support | 2007 |
| Flec Holdings Limited | Financial services | 2020 |
| Fleet Interaction Limited | Administrative & support | 2006 |
| Fleet Rewards Ltd | Administrative & support | 2024 |
| Fleethawk Limited | Information & communication | 2025 |
| Fleming Architects Ltd | Professional & scientific | 2013 |
| Fletcher Mechanical Ltd | Construction | 2018 |
| Fletcher Risk Consultants Ltd | Information & communication | 2024 |
| Fleur DE LYS Bampton Ltd | Hospitality | 2021 |
| Flewitt Consulting Limited | Professional & scientific | 2023 |
| Flex Decals Ltd | Professional & scientific | 2024 |
| Flexfurn Limited | Manufacturing | 1997 |
| Flexipack Ltd | Wholesale & retail | 2015 |
| Flexpay Limited | Financial services | 2013 |
| Flexplans Limited | Financial services | 2016 |
| Flightpartner Limited | Transport & logistics | 2006 |
| Flipfitfinds Ltd | Wholesale & retail | 2025 |
| Flipside Records Ltd | Information & communication | 2020 |
| Flodis Ltd | Professional & scientific | 2021 |
| Flood Avoidance System Ltd | Wholesale & retail | 2011 |
| Flood Painters & Decorators Limited | Construction | 2015 |
| Floods Management Services Ltd | Professional & scientific | 2018 |
| Floorline (Cheltenham) Limited | Wholesale & retail | 2011 |
| Floors ARE US Ltd | Construction | 2014 |
| Floreo One Limited | Construction | 2025 |
| Florin Capital Limited | Financial services | 2018 |
| Flourish Project C.I.C. | Information & communication | 2018 |
| Flow Civil Engineering Ltd | Construction | 2020 |
| Flow SW Ltd | Construction | 2023 |
| Flow-Soul Ltd | Professional & scientific | 2019 |
| Flow-Trade Investments Limited | Financial services | 2023 |
| Flowban Limited | Manufacturing | 2007 |
| Flowerdale Property Management Limited | Real estate | 2002 |
| Flowstate Creative Limited | Information & communication | 2023 |
| Floyd Developments Ltd | Construction | 2025 |
| Fluent Facilities Management Limited | Administrative & support | 2023 |
| Flux Pilates Limited | Arts & recreation | 2025 |
| FLY2HELP | Professional & scientific | 2006 |
| Flycycle Ltd | Administrative & support | 2020 |
| Flykartel Limited | Wholesale & retail | 2016 |
| FM Finance Ltd | Financial services | 2018 |
| FM Ventilation Services Ltd | Wholesale & retail | 2008 |
| Fmcg Export Experts Limited | Wholesale & retail | 2017 |
| FMF Services Ltd | Transport & logistics | 2018 |
| FMS Oral Rehabilitation Limited | Health & social care | 2010 |
| Fnest Properties Ltd | Real estate | 2021 |
| FO365 Contractors Limited | Construction | 2024 |
| Focus Consulting Group Ltd | Professional & scientific | 2016 |
| Focus Development Projects Ltd | Real estate | 2017 |
| Focus Interior Design Limited | Construction | 2014 |
| Focusnetworks-Business LLP | | 2013 |
| FOD Property Group Ltd | Real estate | 2016 |
| FOD Waste Clearance Limited | Water & waste | 2025 |
| Folio Group Consulting Limited | Information & communication | 2024 |
| Folio Group Ltd | Information & communication | 2021 |
| Folio Homes Limited | Construction | 2017 |
| Folly Farm T LLP | | 2010 |
| Folscott Ltd | Hospitality | 2025 |
| Font Consulting Ltd. | Professional & scientific | 2014 |
| Fontworks UK Limited | Wholesale & retail | 1990 |
| FOO GUI Health Limited | Health & social care | 2026 |
| FOO GUI Spa Ltd | Health & social care | 2025 |
| Food Festivals Ltd | Arts & recreation | 2013 |
| Food for Thought 2024 CIC | Professional & scientific | 2024 |
| Food Sovereignty Ltd | Agriculture & fishing | 2019 |
| Foodfarm Agribusiness Limited | Manufacturing | 2025 |
| Foodies Cheltenham Limited | Hospitality | 2026 |
| Foodswingss Ltd | Hospitality | 2025 |
| Football FUN Factory (Community 115) Ltd | Arts & recreation | 2024 |
| Football Trustee Limited | Professional & scientific | 2024 |
| Footsteps Advisory Ltd | Professional & scientific | 2014 |
| FOR Better for Worse | Information & communication | 2020 |
| FOR Every Cloud Ltd | Professional & scientific | 2021 |
| FOR the Brave Ltd | Professional & scientific | 2025 |
| Foran Property Ltd | Real estate | 2022 |
| Forar Ltd | Professional & scientific | 2021 |
| Forbes George Ltd | Administrative & support | 2013 |
| Forbidden Fruits Limited | Wholesale & retail | 2024 |
| Force 8 (Business Services) Limited | Financial services | 2014 |
| Ford Farm Cottages Management Limited | Real estate | 1985 |
| Ford Flooring Limited | Construction | 2003 |
| Forest Cottage Holidays Limited | Real estate | 2021 |
| Forest Estate Hydro Limited | Energy | 2012 |
| Forest of Dean Stone Firms Limited | Mining & quarrying | 2006 |
| Forest of Dean Tyres Limited | Wholesale & retail | 1991 |