| Euphorium Bakery Company Limited | Hospitality | 2016 |
| Euphrates Search Ltd | Administrative & support | 2025 |
| Eurasia (Cheltenham) Ltd | Hospitality | 2011 |
| Eureka Private Limited | Information & communication | 2024 |
| Euro Auto UK Limited | Wholesale & retail | 2021 |
| Euro Cash Register Limited | Wholesale & retail | 2002 |
| Euro Collimators Limited | Manufacturing | 2010 |
| Euroblues Promotions Limited | Arts & recreation | 2005 |
| Eurocars (Cheltenham) Limited | Wholesale & retail | 2009 |
| Eurolink Security Installations Limited | Administrative & support | 2001 |
| Europa Entertainment Services Ltd | Professional & scientific | 2013 |
| Europa Safety Products Limited | Extraterritorial | 1998 |
| Euroserve (Chester) Limited | Hospitality | 1998 |
| Eurox Life Sciences (UK) Limited | Health & social care | 2019 |
| EV Designs Ltd | Manufacturing | 2021 |
| EV Evolution Limited | Professional & scientific | 2023 |
| EVA Rarities Limited | Wholesale & retail | 2015 |
| Evans & Wilkinson Investments Limited | Real estate | 2023 |
| Evans Jones Ltd | Professional & scientific | 2006 |
| Evanson Limited | Information & communication | 2024 |
| EVC & IWD Property Limited | Real estate | 2026 |
| EVE Building Consultancy Limited | Professional & scientific | 2013 |
| Eveg Limited | Professional & scientific | 2025 |
| Evelyn Business Services Limited | Professional & scientific | 2018 |
| Evelyn Court (Cheltenham) Limited | Households | 1959 |
| Evenlode Films and Productions Ltd | Information & communication | 2022 |
| Event & Management Services Limited | Arts & recreation | 2001 |
| Event Automotive Limited | Administrative & support | 2025 |
| Event Craft Solutions Ltd | Administrative & support | 2020 |
| Event LOG Limited | Information & communication | 2017 |
| Eventbase Ltd | Construction | 2012 |
| Eventec Limited | Construction | 2005 |
| Eventl Limited | Wholesale & retail | 2024 |
| Events Dept Ltd | Administrative & support | 2025 |
| Eventwise Limited | Information & communication | 2019 |
| Ever Clean Crew Ltd | Administrative & support | 2026 |
| Everboom Ltd | Wholesale & retail | 2024 |
| Everclean Exterior Solutions Ltd | Administrative & support | 2025 |
| Evergreen Telecom Ltd | Information & communication | 2011 |
| Evergreen Timber & Flooring Limited | Wholesale & retail | 2023 |
| Everlast Industrial Roofing Ltd | Construction | 2023 |
| Evermile U.K. Limited | Administrative & support | 2022 |
| Everrise Property Group Ltd | Construction | 2024 |
| Everwag Ltd | Wholesale & retail | 2025 |
| Everwood Holdco Limited | Administrative & support | 2017 |
| Every Cloud Play & Creative Arts Therapy C.I.C. | Health & social care | 2019 |
| Everyone Swims CIC | Education | 2025 |
| Everything Counts Ltd | Administrative & support | 2024 |
| Evesham Aesthetics Clinic Limited | Other services | 2023 |
| Evesham Signworks Ltd | Professional & scientific | 2024 |
| Evgen Tattoo Art Limited | Arts & recreation | 2025 |
| Evidence Enabled Ltd | Professional & scientific | 2024 |
| Evident Nutrition Limited | Health & social care | 2026 |
| Evinced UK Ltd | Administrative & support | 2025 |
| Evolution Electrical Contracting (UK) Limited | Construction | 2013 |
| Evolution Park Homes and Lodges Limited | Hospitality | 2014 |
| Evolution Property Investments Ltd | Real estate | 2023 |
| Evolve Hub Ltd | Education | 2026 |
| EVOLVE22 Ltd | Professional & scientific | 2026 |
| EVX One Ltd | Information & communication | 2021 |
| EWI Consultants Limited | Administrative & support | 2020 |
| Ewrg Ltd | Health & social care | 2026 |
| Exabeam UK Limited | Information & communication | 2015 |
| Exbeerience Limited | Hospitality | 2017 |
| Exceed Corporate Events Ltd | Administrative & support | 2020 |
| Executive JET Charter Limited | Transport & logistics | 1994 |
| Exela Limited | Administrative & support | 2001 |
| Exodus Game Development Ltd | Information & communication | 2026 |
| Expeditions Living Ltd | Health & social care | 2012 |
| Experience Consulting Limited | Professional & scientific | 2000 |
| Experimental Element Ltd | Wholesale & retail | 2021 |
| Expert Eyebrows Limited | Other services | 2015 |
| Explore Management Limited | Professional & scientific | 2023 |
| Exploring Communities C.I.C. | Health & social care | 2019 |
| Exploring Education (UK) Ltd | Education | 2018 |
| Explotec Limited | Professional & scientific | 2017 |
| Express Fire Protection Limited | Other services | 2008 |
| Express Freight Logistics Ltd | Transport & logistics | 2020 |
| Express Property Services Limited | Construction | 2002 |
| Extract Finance Consulting Ltd | Professional & scientific | 2026 |
| Extrapatch Property Management Limited | Households | 1991 |
| Extreme Environments Limited | Professional & scientific | 2014 |
| Extrucut Aluminium Limited | Manufacturing | 2012 |
| Extrucut DIE Limited | Manufacturing | 2019 |
| Extrusion Form Tools Limited | Manufacturing | 1977 |
| EYE Surgery Cheltenham Ltd | Health & social care | 2023 |
| EYECANDY2 Limited | Professional & scientific | 2026 |
| Eyerhymer Limited | Construction | 2020 |
| Eyksu Healthcare Services Ltd | Administrative & support | 2013 |
| Eyre Plastering and Building Services Limited | Construction | 2021 |
| EYS Elkstone Ltd | Health & social care | 2026 |
| EYS Leamington Spa Ltd | Health & social care | 2025 |
| EZ Money Marketing Ltd | Professional & scientific | 2025 |
| Ezek Consulting Limited | Professional & scientific | 1998 |
| F & M Building Ltd | Construction | 2023 |
| F & M Plumbing & Heating Ltd | Construction | 2020 |
| F & R Light Park Homes Limited | Real estate | 2014 |
| F J Advisory Ltd | Professional & scientific | 2021 |
| F Long Consulting Limited | Professional & scientific | 2023 |
| F Murphy Electrical Services Ltd | Construction | 2025 |