| Autism Assessment Centre Ltd | Extraterritorial | 2023 |
| Auto Boats Limited | Manufacturing | 2020 |
| Auto Emotive Design Limited | Information & communication | 2012 |
| Auto Network UK Limited | Administrative & support | 1999 |
| Auto Wiring Limited | Construction | 2006 |
| Auto-Enrolment Bureau Ltd | Administrative & support | 2013 |
| Auto-Tek Repairs Ltd | Wholesale & retail | 2019 |
| Autobahn Car Centre Ltd | Wholesale & retail | 2023 |
| Autocheck Car Centre Limited | Wholesale & retail | 2005 |
| Autoclenz EOT Limited | Financial services | 2025 |
| Autocrafts (Stroud) Limited | Real estate | 1961 |
| Autofour Precision Engineering Limited | Manufacturing | 1985 |
| Autohaus Cheltenham Ltd | Wholesale & retail | 2024 |
| Autoownershub Limited | Professional & scientific | 2026 |
| Autotec Classic Cars Ltd | Wholesale & retail | 2013 |
| Autovillage (Cheltenham) Limited | Wholesale & retail | 2005 |
| Autovip Cheltenham Ltd | Wholesale & retail | 2024 |
| Autumn Ventures Limited | Real estate | 2025 |
| Auwell Electronics Limited | Manufacturing | 1977 |
| Auwell Holdings Limited | Financial services | 2026 |
| Auxil Limited | Professional & scientific | 2014 |
| Auxillias Limited | Professional & scientific | 2020 |
| AV Chohan Ltd | Financial services | 2026 |
| Avaglade Limited | Real estate | 2025 |
| Avalon Roots Limited | Education | 2025 |
| Avant Garde Hereford Limited | Other services | 2017 |
| Avant Garde Tattoo Ltd | Other services | 2020 |
| Avant Garden Services Ltd | Administrative & support | 2023 |
| Avasr Cloud Ltd | Information & communication | 2024 |
| Avcorp Services Limited | Administrative & support | 2017 |
| Avenue Stow Ltd | Wholesale & retail | 2024 |
| Averly Devco Limited | Construction | 2025 |
| Averly Project 1 Limited | Construction | 2025 |
| Averly Trading Limited | Financial services | 2025 |
| Avery Tech Ltd | Information & communication | 2026 |
| Avezika Limited | Hospitality | 2007 |
| Aviarm Ltd | Professional & scientific | 2021 |
| Aviation Management Services Limited | Professional & scientific | 2008 |
| Aviation Solutions International (UK) Ltd | Administrative & support | 2018 |
| Aviator Inn Ltd | Hospitality | 2005 |
| Avid Events Limited | Professional & scientific | 2011 |
| Avilon Property Management Limited | Construction | 2015 |
| Avinra Travels Private Limited | Administrative & support | 2025 |
| Aviso Consulting Limited | Professional & scientific | 2012 |
| Avitrius Air International Limited | Transport & logistics | 2024 |
| Avitrius Aviation Limited | Transport & logistics | 2026 |
| Avitrius Group Holdings Limited | Financial services | 2026 |
| Avixa UK Limited | Administrative & support | 2019 |
| AVM International Ltd. | Transport & logistics | 1997 |
| AVM Logistics Holdings Limited | Financial services | 2014 |
| AVM Storage & Removals Ltd | Transport & logistics | 2009 |
| AVM Storage Ltd | Extraterritorial | 2010 |
| Avnconsulting Ltd | Professional & scientific | 2026 |
| Avondale (Cheltenham) Management Company Limited | Households | 1988 |
| Avonmouth Pharmacy Limited | Health & social care | 2002 |
| Avosolution Ltd | Professional & scientific | 2025 |
| AVS Consultancy Limited | Administrative & support | 2007 |
| AW Fencing and Driveways Limited | Administrative & support | 2003 |
| Awake Community Ltd | Wholesale & retail | 2025 |
| AWB Financial Ltd | Financial services | 2022 |
| Awfully Limited | Professional & scientific | 2014 |
| AWK Holdings Ltd | Financial services | 2016 |
| AWP Creative Ltd | Professional & scientific | 2015 |
| AXE Nomads Ltd | Arts & recreation | 2021 |
| Axelerants Ltd | Professional & scientific | 2019 |
| Axensus Limited | Professional & scientific | 1998 |
| Axessibility Ltd | Administrative & support | 2016 |
| Axicron Technologies Ltd | Information & communication | 2026 |
| Axiom BS Holdings Ltd | Financial services | 2023 |
| AXJ Trading Ltd | Wholesale & retail | 2018 |
| Axolotl Cyber Security Limited | Information & communication | 2025 |
| AXP Limited | Construction | 2026 |
| Axyron PVT Ltd | Wholesale & retail | 2026 |
| Ayce Planning Limited | Extraterritorial | 2016 |
| Ayrita Ltd | Real estate | 2024 |
| Ayush Abilash Ltd | Health & social care | 2019 |
| Azimuth Associates Limited | Professional & scientific | 2009 |
| Azimuth Structural Engineering Ltd | Construction | 2004 |
| Aziraph Ltd | Information & communication | 2026 |
| Azran Limited | Professional & scientific | 2006 |
| Aztec Industrial Safety LLP | | 2004 |
| Azurar Consultants Ltd. | Information & communication | 1993 |
| Azure Harvest Limited | Professional & scientific | 2021 |
| B & M Babbage (Midlands) Limited | Construction | 2006 |
| B & M Babbage Investments Limited | Real estate | 2021 |
| B & R Plastering Limited | Construction | 2015 |
| B & W Hire Limited | Administrative & support | 2006 |
| B 13 B Unlimited | Financial services | 2022 |
| B and S PUB Co Ltd | Hospitality | 2018 |
| B Appah Electricals Ltd | Construction | 2019 |
| B B Timber Limited | Agriculture & fishing | 1998 |
| B E Rudman Drives & Gardens Ltd | Construction | 2026 |
| B Gifted Ltd | Wholesale & retail | 2010 |
| B Hunt Electrical Limited | Construction | 2008 |
| B K Fencing Ltd | Construction | 2000 |
| B K Properties (Glos) Limited | Real estate | 2024 |
| B L C Fabrications Ltd | Manufacturing | 2001 |
| B P E Secretaries Limited | Professional & scientific | 1984 |
| B S Property Development Ltd | Construction | 2004 |
| B Stop Ltd | Wholesale & retail | 2026 |