| Studio Coffee Roasters Ltd | Manufacturing | 2017 |
| Studio Cotswold BED Company Limited | Manufacturing | 2023 |
| Studio II Limited | Professional & scientific | 1999 |
| Studio RAY Limited | Professional & scientific | 2024 |
| Studio Sorores Ltd | Wholesale & retail | 2017 |
| Studio Thirteen Limited | Financial services | 2015 |
| Studiofit Cheltenham Ltd | Arts & recreation | 2018 |
| Studiofit Group Ltd | Arts & recreation | 2018 |
| Studiofit PT Ltd | Arts & recreation | 2018 |
| Studytimes Limited | Wholesale & retail | 2000 |
| Sturdy Kids Ltd | Wholesale & retail | 2013 |
| Style & Order Ltd | Other services | 2022 |
| Stylesworks Ltd | Information & communication | 2022 |
| SUB OMG Ltd | Hospitality | 2018 |
| Sub-Distribution Limited | Manufacturing | 2016 |
| Subjunctive Limited | Information & communication | 2011 |
| Sublime Superbikes Ltd | Wholesale & retail | 2025 |
| Sudley Property Ltd | Real estate | 2024 |
| Suesonglos Limited | Households | 2020 |
| Suffand Residents Association Limited | Households | 1982 |
| Suffco Ltd | Real estate | 2025 |
| Suffolk Coffee (Cheltenham) Ltd | Hospitality | 2021 |
| Suffolk Court Management Company Limited | Households | 1988 |
| Suffolk House (Cheltenham) Limited | Households | 1985 |
| Suffolk Road Limited | Real estate | 2019 |
| Suffolk Square Care Limited | Real estate | 2012 |
| Sugar Town Inc Limited | Extraterritorial | 2020 |
| Sugarai UK Limited | Information & communication | 2005 |
| Sugarcrm UK Limited | Information & communication | 2007 |
| Sugarlumps Limited | Professional & scientific | 2015 |
| Sukjai Trading Limited | Arts & recreation | 2019 |
| Sumit Geospace Ltd | Arts & recreation | 2026 |
| Summed Ltd | Other services | 2025 |
| Summer Network Solutions Limited | Professional & scientific | 2023 |
| Summerfield Commercials Limited | Real estate | 1993 |
| Summerfield House (Management) Company Limited | Households | 2000 |
| Summerhill Equine Veterinary Partnership LLP | | 2009 |
| Summers Developments Ltd | Construction | 2020 |
| Summerspring Residents Association Limited | Households | 1983 |
| Summit Computer Recruitment Limited | Administrative & support | 1999 |
| Summit Medical (Benefits) Limited | Manufacturing | 1984 |
| Summit Medical Group Limited | Manufacturing | 2008 |
| Summit Medical Limited | Manufacturing | 2001 |
| Sumner Court Management Company Limited | Households | 2005 |
| Sumologic Limited | Information & communication | 2014 |
| Sunbury Computing Services Limited | Information & communication | 1988 |
| Sunde Apparel Ltd | Manufacturing | 2023 |
| Sundridge (Cheltenham) Limited | Hospitality | 1983 |
| Sunfixings Limited | Manufacturing | 2011 |
| Sunflower Event Production Limited | Information & communication | 2005 |
| Sunningend Ltd | Real estate | 2019 |
| Sunvelo Limited | Administrative & support | 2012 |
| Supafoot Cheltenham Limited | Health & social care | 2017 |
| Supastrikersrg Limited | Education | 2021 |
| Super Property Developments Ltd | Real estate | 2020 |
| Super Training Events Limited | Arts & recreation | 2021 |
| Superdry EUR Finance Ltd | Financial services | 2023 |
| Superdry Limited | Wholesale & retail | 2009 |
| Superfish Holdings Limited | Professional & scientific | 2024 |
| Supergroup Concessions Limited | Wholesale & retail | 2010 |
| Supergroup Internet Limited | Wholesale & retail | 2010 |
| Supernannies Ltd | Professional & scientific | 2024 |
| Superstand Limited | Real estate | 2003 |
| Supp to YOU Ltd | Wholesale & retail | 2021 |
| Supply Pro Services Ltd | Professional & scientific | 2025 |
| Support by DAN Ltd | Extraterritorial | 2025 |
| Suppose Ltd | Information & communication | 2008 |
| Supreme Cars (Cheltenham) Ltd | Wholesale & retail | 2013 |
| Surelet - Cheltenham Ltd | Real estate | 2009 |
| Surfleet Limited | Professional & scientific | 2012 |
| Surgical Specialties Corporation Limited | Manufacturing | 2017 |
| Surgical Specialties UK Holdings Limited | Manufacturing | 2004 |
| Surma Housing Ltd | Real estate | 2025 |
| Surpic Limited | Information & communication | 2011 |
| Surrey Maintenance & Cleaning Ltd | Administrative & support | 2025 |
| Surrey Orthodontics Limited | Health & social care | 2011 |
| Susan Healey Property Consultancy Ltd | Real estate | 2016 |
| Susan Molyneux Cosmetics Limited | Manufacturing | 1988 |
| Sushi & Peri Ltd | Hospitality | 2026 |
| Sussex Mews Management Limited | Households | 2016 |
| Sustainability Consulting Solutions Limited | Professional & scientific | 2010 |
| Sustainable Business Finance Ltd | Financial services | 2024 |
| Sustainable Electrical Supply Limited | Professional & scientific | 2012 |
| Sustainable Marketing Ltd | Professional & scientific | 2021 |
| Sustainable Viability Holdings Ltd | Financial services | 2025 |
| Sustainable Viability Limited | Professional & scientific | 2019 |
| Sutcon Civils Limited | Construction | 2025 |
| Sutherland Associates Limited | Health & social care | 2014 |
| Sutherland Education Group Limited | Extraterritorial | 2015 |
| Sutherland Education Ltd | Education | 1997 |
| Sutlow Dobbs Associates Limited | Construction | 2000 |
| Sutton Building & Developments Ltd | Construction | 2017 |
| Sutton COX Architects Limited | Professional & scientific | 2019 |
| Sutton Plant (Glos) Ltd | Construction | 2023 |
| Suxeed Ltd | Professional & scientific | 2024 |
| Suyi Caresure Limited | Health & social care | 2023 |
| Sv-Electra Ltd | Professional & scientific | 2024 |
| SVR Chem (Holdings) Ltd | Financial services | 2016 |
| SVR Investment (Holdings) Ltd | Financial services | 2016 |
| SVR Investment Ltd | Administrative & support | 2016 |