| 127 London Road (Chatteris) Limited | Households | 2002 |
| 25 Station Road Management Company Limited | Administrative & support | 2004 |
| 381 Investments Limited | Financial services | 2010 |
| 54 Bridge Street Management Chatteris Ltd | Households | 2019 |
| 9STEP Limited | Manufacturing | 2016 |
| A & S Hearing Services Ltd | Health & social care | 2003 |
| A Game of Bones Ltd | Hospitality | 2019 |
| A K Builders & Plumbers Ltd | Construction | 2017 |
| A R Structurals Ltd | Manufacturing | 2019 |
| A&G Pet Holdings Ltd | Financial services | 2025 |
| A&M Farming Ltd | Agriculture & fishing | 2023 |
| A. Kelway Ltd | Manufacturing | 2008 |
| A.J.W. Distribution Limited | Wholesale & retail | 1998 |
| A.N Delivery Ltd | Construction | 2023 |
| Aagia's Company Limited | Health & social care | 2019 |
| Abuzer Construction Ltd | Construction | 2021 |
| AC Salvage Ltd | Wholesale & retail | 2026 |
| ACD Contractors Ltd | Construction | 2024 |
| ACD Servicing Ltd | Wholesale & retail | 2020 |
| ACG Medical Limited | Information & communication | 2013 |
| ACS Beasley Ltd | Real estate | 2022 |
| Adek Management Limited | Professional & scientific | 2006 |
| Advanced Precision Technologies Ltd | Professional & scientific | 2021 |
| Aetherx Ltd | Wholesale & retail | 2025 |
| Aichinger Films Limited | Information & communication | 2018 |
| Aichmoore Aquatics Limited | Wholesale & retail | 2016 |
| AJW Distribution (Cambridge) Ltd | Wholesale & retail | 2014 |
| AJW Distribution (Essex) Limited | Wholesale & retail | 2007 |
| AJW Distribution (Norwich) Limited | Wholesale & retail | 2002 |
| AJW Group Limited | Financial services | 2002 |
| AJW Industrial Roofing and Cladding Supplies Ltd | Wholesale & retail | 2018 |
| AL Property Hub Limited | Real estate | 2024 |
| AL Timber Ltd | Wholesale & retail | 2014 |
| ALI Mcnamara Limited | Arts & recreation | 2014 |
| ALL Seasons Childrens Play CIC | Health & social care | 2022 |
| Allgood Products Limited | Wholesale & retail | 2012 |
| Alloriental Ltd | Wholesale & retail | 2023 |
| Allpress Farms Limited | Agriculture & fishing | 1965 |
| Alphamsp Ltd | Professional & scientific | 2022 |
| Ambers Cleaning Services Ltd | Administrative & support | 2026 |
| Andak Catering Ltd | Hospitality | 2021 |
| Aoife Doherty Design Limited | Information & communication | 2016 |
| AP Wireworx Ltd | Manufacturing | 2024 |
| Apex Coffee Ltd | Hospitality | 2019 |
| Apholman Limited | Wholesale & retail | 2021 |
| Aquarist Marketplace Ltd | Wholesale & retail | 2023 |
| Araya Spice Ltd | Hospitality | 2025 |
| ARR Creations Ltd | Manufacturing | 2024 |
| Arrowsmith Medical Services Ltd | Health & social care | 2018 |
| Artronics Manufacturing Limited | Manufacturing | 1997 |
| Ascentrik Ltd | Construction | 2018 |
| ASK Jordon Ltd | Professional & scientific | 2022 |
| Askham Farming Ltd | Construction | 2017 |
| Atlantic Halls Limited | Construction | 1986 |
| Atman - the International Federation of Yoga and Meditation | Arts & recreation | 2004 |
| Avian Properties Limited | Real estate | 2005 |
| Avingtrans PLC | Professional & scientific | 1985 |
| Azzy Land Ltd | Wholesale & retail | 2025 |
| B + K Augustin Limited | Manufacturing | 2004 |
| Bali Beds Ltd | Other services | 2024 |
| Barnes Gardens Chatteris Management Company Limited | Professional & scientific | 2010 |
| Barry Jackson Transport Limited | Transport & logistics | 2003 |
| Bartlett Co. (Chatteris) Limited | Real estate | 2009 |
| Bartlett Co. (Gooderstone) Limited | Extraterritorial | 2001 |
| BDS GRP Ltd | Wholesale & retail | 2022 |
| Beaneymx Coaching Limited | Education | 2025 |
| Bella ORA Salon Ltd | Other services | 2024 |
| Belton Developments & Construction Ltd | Real estate | 2023 |
| Besides CAD Limited | Information & communication | 2007 |
| Besides Homes Limited | Real estate | 2022 |
| Bios Analytical Limited | Wholesale & retail | 2013 |
| Bios Analytique Ltd | Administrative & support | 2009 |
| BMB Electrical Limited | Construction | 2004 |
| BMG Customs Clearance Ltd | Transport & logistics | 2021 |
| Body Mind and Soul Holistic Limited | Health & social care | 2016 |
| Booth Industries International Limited | Manufacturing | 2021 |
| Bowella Recruit Limited | Administrative & support | 2019 |
| Bradley Signature Homes Ltd | Real estate | 2023 |
| Brain Body Wealth Ltd | Health & social care | 2017 |
| Brett Rigby Ltd | Information & communication | 2018 |
| Bridge House Cafe Ltd | Hospitality | 2021 |
| Britania HCW Ltd | Other services | 2015 |
| Brookgate Holdings Limited | Professional & scientific | 2022 |
| Bsli Renovations Ltd | Construction | 2022 |
| Buchanan Construction (Cambridge) Limited | Construction | 2020 |
| Buffalo Trades Limited | Health & social care | 2015 |
| Business Ready Ltd | Professional & scientific | 2021 |
| Butler's Resin & Construction Ltd | Construction | 2024 |
| BUY4LESS Groceries Limited | Wholesale & retail | 2023 |
| Buzzvue Ltd | Information & communication | 2020 |
| BZ Enterprises Ltd | Construction | 2021 |
| C & G Coach Services Limited | Transport & logistics | 2001 |
| Café 42 Chatteris Ltd | Hospitality | 2023 |
| Cambridge Courier Services Ltd | Transport & logistics | 2001 |
| Cambridge Party Shop Limited | Wholesale & retail | 2016 |
| Cambridgeshire Pest and Wildlife Management Ltd | Administrative & support | 2024 |
| Cameo Curtains and Blinds Limited | Manufacturing | 2010 |
| Cameo Waste Solutions Limited | Water & waste | 2020 |
| Cameron Brook & Associates Limited | Administrative & support | 2004 |
| Campbell Brickwork Ltd | Construction | 2022 |