| Drumyard Limited | Real estate | 1992 |
| DS Expert Builders Ltd | Construction | 2014 |
| DSC Building & Development Services Ltd | Construction | 2024 |
| DSC Consultancy (Kent) Ltd | Construction | 2019 |
| DSM Leaseholds Ltd | Real estate | 2026 |
| DSM Media Ltd | Professional & scientific | 2015 |
| DSM Property Rentals Ltd | Real estate | 2023 |
| DSY Capital Ltd | Real estate | 2026 |
| Dttk Frontiers Ltd | Health & social care | 2026 |
| Dttwo Ventures Ltd | Health & social care | 2020 |
| Dudu MC Ltd | Transport & logistics | 2020 |
| Duncan Collins Ltd | Administrative & support | 2016 |
| Dunn AC Limited | Manufacturing | 2010 |
| Durant Lifts Limited | Professional & scientific | 2023 |
| Durshamwood Management Company Limited | Households | 1982 |
| Durus Kebab House Ltd | Hospitality | 2024 |
| DVS Electrical Services Ltd | Construction | 2023 |
| Dwell Property Group Ltd | Real estate | 2026 |
| Dylan Brown Ltd | Construction | 2022 |
| Dymphna D'Costa Consultancy Limited | Other services | 2017 |
| Dymphna Ines D’Costa Consultancy Limited | Professional & scientific | 2026 |
| Dynamic Care Hub Limited | Education | 2024 |
| Dyneema Limited | Construction | 2009 |
| Dyson Brown Limited | Other services | 2002 |
| Dyson Brown Gent's Hairdressing Limited | Other services | 2010 |
| Dyson Brown Tenterden Limited | Other services | 2011 |
| DZT Transport Ltd | Transport & logistics | 2026 |
| E Anaafi Ltd | Construction | 2015 |
| E Faraday Group Limited | Construction | 2020 |
| E H Properties Limited | Real estate | 1989 |
| E H Therapy Clinic Ltd | Health & social care | 2026 |
| E W Ballard (1993) Limited | Professional & scientific | 1993 |
| E&A Plumbing and Heating Ltd | Construction | 2024 |
| E&D Property Investments Ltd | Real estate | 2019 |
| E&R Chatham Limited | Hospitality | 2025 |
| E&Tj UK Limited | Wholesale & retail | 2021 |
| E-Luxe Limited | Hospitality | 2017 |
| E-Mail Guardian Limited | Information & communication | 2016 |
| E-Nnovation.Com Limited | Information & communication | 1994 |
| E-Scape Gardens Limited | Administrative & support | 2013 |
| E.B.G. (Helicopters) Limited | Transport & logistics | 1999 |
| E.J. Crush Limited | Transport & logistics | 2014 |
| E.W. Ballard Limited | Real estate | 1949 |
| E2 Electrical Solutions Ltd | Construction | 2024 |
| E2E Supply Chain Solutions Ltd | Professional & scientific | 2011 |
| Eagle Kent Ltd | Hospitality | 2025 |
| Ean-H Ltd | Wholesale & retail | 2014 |
| Eaosolutions Ltd | Information & communication | 2022 |
| Earblast Limited | Professional & scientific | 2011 |
| Earls Properties Ltd | Hospitality | 2025 |
| Earth Resources Group Ltd | Professional & scientific | 2017 |
| Easioption Limited | Financial services | 2002 |
| East Kent Subaru Ltd | Wholesale & retail | 2016 |
| East Kent Windows & Doors Ltd | Extraterritorial | 2018 |
| East London Property Solutions Ltd | Real estate | 2023 |
| Easton Heating and Plumbing Ltd | Construction | 2022 |
| Easy Deal Techie Ltd | Information & communication | 2018 |
| Easy Installations Ltd | Construction | 2021 |
| Easy Street Estate Ltd | Real estate | 2022 |
| Easyforward Limited | Real estate | 1986 |
| Eavee AI Limited | Information & communication | 2024 |
| Eazy Rentals Ltd | Real estate | 2018 |
| Eba-Cast Properties Limited | Real estate | 2025 |
| Ebbsfleet Automotive Ltd | Wholesale & retail | 2020 |
| Ebbsfleet Fried Chicken Ltd | Hospitality | 2024 |
| Ebbsfleet Transport Limited | Transport & logistics | 1995 |
| EBG Helicopters (Sales & Maintenance) Limited | Administrative & support | 2010 |
| Ebic Limited | Wholesale & retail | 2016 |
| Ebm-R Solution Ltd | Other services | 2024 |
| Ebony Aura Ltd | Other services | 2026 |
| Ebrey Limited | Other services | 2011 |
| Ebury Homes Limited | Real estate | 2022 |
| EC Carpentry Ltd | Construction | 2017 |
| EC Safety Solutions Limited | Professional & scientific | 2009 |
| Eccentric Properties Limited | Real estate | 2023 |
| Echemule Limited | Real estate | 2023 |
| Echo Facilities Management Ltd | Administrative & support | 2022 |
| Echo Fulfilment Limited | Transport & logistics | 2026 |
| Echo Meds Limited | Health & social care | 2020 |
| Echo Plumbing Limited | Construction | 2007 |
| Echo Support Services Ltd | Administrative & support | 2013 |
| Eclectic Investment Group Ltd | Financial services | 2015 |
| Eclessia Romany International Bible School Ltd | Other services | 2025 |
| Eclips Enterprise Ltd. | Financial services | 2003 |
| ECM Global Solutions Ltd | Information & communication | 2024 |
| Eco Design and Build Solutions Limited | Wholesale & retail | 2006 |
| Eco Elite Limited | Administrative & support | 2009 |
| Eco Green Building Ltd | Construction | 2024 |
| Eco Resolve Limited | Administrative & support | 2019 |
| Eco-Dent Supplies Ltd | Manufacturing | 2024 |
| Econexec Limited | Administrative & support | 2022 |
| Ecrotex Limited | Construction | 2014 |
| ED and James Limited | Manufacturing | 2023 |
| Eddiejide-Net Ltd | Information & communication | 2023 |
| Eddybond International Ltd | Manufacturing | 2018 |
| Eden Associates (UK) Limited | Wholesale & retail | 1998 |
| Eden Contract Services Limited | Professional & scientific | 2026 |
| Eden GRP Limited | Construction | 2021 |
| Eden Software Limited | Information & communication | 2020 |
| Edenlight Housing and Support Ltd | Hospitality | 2024 |