| Sarcastic Hedgehog Limited | Information & communication | 2011 |
| SAY IT With Flowers Ltd | Wholesale & retail | 2020 |
| Scopemaster Limited | Information & communication | 2018 |
| Scott Product Leaders Ltd | Information & communication | 2024 |
| SDS Legal Limited | Administrative & support | 2018 |
| Semca Ltd | Information & communication | 1997 |
| SET 2 SET Limited | Transport & logistics | 2016 |
| Seven Thirty Nine Media Ltd | Professional & scientific | 2025 |
| SF Site Management Services Limited | Professional & scientific | 2016 |
| SH2 Consulting Ltd | Professional & scientific | 2010 |
| Shaw Feltham Limited | Real estate | 2026 |
| Shibuso Limited | Information & communication | 2017 |
| Silsden Properties Limited | Construction | 1965 |
| Singlewise Limited | Real estate | 1982 |
| Solarity Limited | Energy | 2025 |
| South Bank Photographic Services Limited | Professional & scientific | 1999 |
| Sri's ST Giles Store Ltd | Wholesale & retail | 2025 |
| ST Giles Expert Services Ltd | Professional & scientific | 2021 |
| ST Giles Furniture Ltd | Wholesale & retail | 2002 |
| Stillpoint Ventures Ltd. | Manufacturing | 2024 |
| Strathyre Holdings Limited | Professional & scientific | 2025 |
| Stratton Chase Drive Company Limited | Real estate | 1976 |
| Strawberry Digital Limited | Information & communication | 2025 |
| Studio Solutions UK Ltd | Information & communication | 2023 |
| Superb Energy Ltd | Professional & scientific | 2022 |
| Sweet LE Roux Ltd | Manufacturing | 2025 |
| Sword and Trowel Roofing Ltd | Construction | 2025 |
| TAG Exclusive Properties Urban Limited | Construction | 2014 |
| Taplow Developments Limited | Construction | 2018 |
| Teamsale Limited | Real estate | 1987 |
| Thakerar Partnership Limited | Professional & scientific | 2011 |
| THE Beauty Garden Bovingdon Ltd | Other services | 2007 |
| THE Boom Boom Bar Limited | Hospitality | 2015 |
| THE Cambridge London Group Limited | Professional & scientific | 2005 |
| THE Cambridge London Partnership Limited | Professional & scientific | 2005 |
| THE Cambridge London Trading Company Limited | Professional & scientific | 2005 |
| THE Grumpy Collective Ltd | Construction | 2023 |
| THE Human Made Mark Ltd | Information & communication | 2025 |
| THE Kitchen Ambassador Limited | Agriculture & fishing | 2016 |
| THE Media Connexions Limited | Professional & scientific | 2013 |
| THE Paddocks Residents Association (Chalfont ST Giles) Limited | Other services | 1991 |
| THE Patch Test KIT Ltd | Other services | 2019 |
| THE Ride Travel Ltd | Administrative & support | 2024 |
| THE Rough-Stuff Fellowship Limited | Arts & recreation | 2000 |
| THE Walled Garden Equine Ltd | Agriculture & fishing | 2021 |
| Thinc Digital Limited | Information & communication | 2018 |
| Think Resourceful Limited | Administrative & support | 2010 |
| Tiger Turnip Limited | Manufacturing | 2020 |
| Tiling Ltd | Other services | 2016 |
| Tinco Limited | Information & communication | 2011 |
| Tommy Custom Builds Ltd | Construction | 2025 |
| Train AI Ltd | Education | 2025 |
| Trapdoor Limited | Arts & recreation | 2010 |
| Trendcap Limited | Financial services | 2016 |
| Trerammet Crescent Management Limited | Real estate | 2020 |
| Twelve the Green Ltd | Other services | 2020 |
| Twenty Seven Letters Ltd | Real estate | 2023 |
| Two Lyons Property Limited | Real estate | 2019 |
| Tyler Gardens Limited | Administrative & support | 2026 |
| Tyler London Limited | Administrative & support | 2026 |
| Tyrenet Limited | Information & communication | 1996 |
| UK Garage Conversions Ltd | Construction | 2026 |
| Ultra Resilience Ltd | Health & social care | 2018 |
| Untangled Management Solutions Ltd | Professional & scientific | 2022 |
| V & R Solutions Ltd | Professional & scientific | 2011 |
| Vache Management Services Limited | Other services | 1998 |
| Valusphere Ltd | Information & communication | 2025 |
| Vickery & Beaumond Limited | Wholesale & retail | 2023 |
| Village Hair & Salon Limited | Other services | 2011 |
| Volks-Tec Ltd | Wholesale & retail | 2011 |
| Vortex Yoga Limited | Arts & recreation | 2025 |
| W.E. Investments Limited | Real estate | 2003 |
| Walbrook Advisors Limited | Professional & scientific | 2015 |
| Walnuts Investments Ltd | Real estate | 2018 |
| Warners at Chalfont Limited | Hospitality | 2011 |
| WE Buy Land Ltd | Construction | 2010 |
| Weldale Investments Limited | Real estate | 1998 |
| West Flank Ltd | Construction | 2024 |
| WKE Services Ltd | Energy | 2010 |
| Worboys & Johnston Properties Limited | Real estate | 2018 |
| Works OUT Best Limited | Professional & scientific | 2016 |
| Wyvern a Limited | Financial services | 2026 |
| Wyvern B Limited | Financial services | 2026 |
| Xivarri Limited | Real estate | 2005 |
| Yacht Fractions Ltd | Wholesale & retail | 2024 |
| Yalden Limited | Wholesale & retail | 2020 |
| YEC Management Limited | Construction | 2017 |
| Young Iron Capital Limited | Financial services | 2023 |
| Zehnder Consulting Services Limited | Professional & scientific | 2017 |