| Demijohn Limited | Wholesale & retail | 2004 |
| DES Saints & Trade Limited | Wholesale & retail | 2025 |
| Discover Scotland (Self-Catering Cottages) Limited | Administrative & support | 1997 |
| Domes by the Sea Limited | Hospitality | 2025 |
| Doodle Trust | Other services | 2010 |
| Douglas Arms Hotel Ltd | Hospitality | 2024 |
| Driftwood Holiday Home Southerness Ltd | Hospitality | 2021 |
| Drumrash Farm Limited Partnership | | 1987 |
| Dumfries Curling Limited | Education | 2018 |
| Dunjop Farm Partners Limited | | 1986 |
| Dunjop Farming Company | | 1989 |
| Dunlop Storage Solutions Limited | Real estate | 2025 |
| Durham Professional Services Limited | Administrative & support | 2013 |
| Dusty Wren Ltd | Manufacturing | 2026 |
| Dx-Uk Group Ltd | Wholesale & retail | 2025 |
| EAS MOR Hydro LLP | | 2015 |
| East Logan Limited | Agriculture & fishing | 2019 |
| EBD Galloway Limited | Manufacturing | 2014 |
| Ecosafety Limited | Public administration | 2006 |
| Edinburgh New Town Hideaways Ltd | Hospitality | 2023 |
| Edwin and Jessies Limited | Other services | 2016 |
| Eibyy Limited | Hospitality | 2012 |
| Elridge Holdings Ltd | Real estate | 2026 |
| Embracing Life Consulting Ltd | Professional & scientific | 2020 |
| Enviroroute Limited | Administrative & support | 2024 |
| Ernespie Farm Centre Limited | Hospitality | 2020 |
| ES Leisure Consultancy Ltd | Professional & scientific | 2020 |
| Evergreen Companions Limited | Health & social care | 2024 |
| Ewep Ltd | Health & social care | 2025 |
| Falco Farm UK Limited | Agriculture & fishing | 2012 |
| Fastrak Motorcycle Training Ltd | Education | 2024 |
| Fearann Alba Ltd | Information & communication | 2019 |
| Fieldfare Associates Northern Limited | Professional & scientific | 2009 |
| Finlay (Waterside) | | 1987 |
| Finlay's Farm Ltd | Manufacturing | 2017 |
| Fireplaces Direct by Robert Nicholson Ltd | Wholesale & retail | 2011 |
| Fleet Health & Safety Limited | Professional & scientific | 2021 |
| Forge & Fern Ltd | Administrative & support | 2025 |
| Forrest Estate Limited | Agriculture & fishing | 1992 |
| Forrest Renewables Limited | Professional & scientific | 1999 |
| FOX and Hound Theatre Ltd | Arts & recreation | 2025 |
| Freshpath Dairies | Agriculture & fishing | 2025 |
| G G & B Nominees Limited | Professional & scientific | 1994 |
| G.S.L Vehicle Sales Ltd | Wholesale & retail | 2025 |
| Galloway Activity Centre Limited | Hospitality | 2015 |
| Galloway Aerial Surveys Limited | Extraterritorial | 2013 |
| Galloway Broadband Limited | Information & communication | 2023 |
| Galloway Building Services Limited | Wholesale & retail | 1997 |
| Galloway Canyoning Ltd | Administrative & support | 2023 |
| Galloway Community Group CIC | Arts & recreation | 2019 |
| Galloway Conservatory Solutions Ltd | Wholesale & retail | 2023 |
| Galloway Cycle Works Ltd | Manufacturing | 2024 |
| Galloway Drainage Supplies Ltd. | Construction | 2014 |
| Galloway Engineering Services Ltd | Construction | 2025 |
| Galloway Fire Safety Services Limited | Professional & scientific | 2013 |
| Galloway Food Hub CIC | Other services | 2024 |
| Galloway GIN Limited | Manufacturing | 2023 |
| Galloway Hideaways Ltd | Hospitality | 2022 |
| Galloway Land and Property Services Limited | Professional & scientific | 1992 |
| Galloway Media Ltd | Information & communication | 2025 |
| Galloway Service and Logistics Ltd | Wholesale & retail | 2022 |
| Gaps Properties Ltd | Real estate | 2021 |
| Gatehouse of Fleet Ymca | Arts & recreation | 2001 |
| GB Barrels Ltd | Manufacturing | 2014 |
| Geezabit Takeaway Ltd | Hospitality | 2026 |
| Geomacs Rentals Limited | Administrative & support | 2008 |
| GET Outdoors Galloway CIC | Arts & recreation | 2026 |
| Gift Engraver Limited | Arts & recreation | 2011 |
| Gillespie Leisure Limited | Hospitality | 1982 |
| Glengunnoch Lodges Limited | Hospitality | 2023 |
| Glenlaggan Lodges Limited | Hospitality | 2008 |
| Graham J Armstrong | | 1992 |
| Gray Tweed Limited | Real estate | 2019 |
| Green Light Experience Ltd | Construction | 2018 |
| Greg Kirkpatrick Electrical Ltd | Construction | 2026 |
| Griffin Freehold Ltd | Hospitality | 2016 |
| Groom Wildlife and Environmental Limited | Agriculture & fishing | 2021 |
| GS Constructions (Borgue) Ltd | Construction | 2012 |
| Habella UK Limited | Wholesale & retail | 2019 |
| Haggerty Holdings Ltd | Professional & scientific | 2025 |
| Halfmark Farm Ltd | Agriculture & fishing | 2022 |
| Halikarnas Scotland Limited | Hospitality | 2024 |
| Hall Baird Solicitors Limited | Professional & scientific | 2015 |
| Halmyre Farming Limited | Agriculture & fishing | 2019 |
| Hanody Limited | Education | 2025 |
| Hardhills Properties Ltd | Professional & scientific | 2020 |
| Helson and Jackets Limited Liability Partnership | | 2013 |
| Hensol Shoot | | 1998 |
| High Barcaple Renewables Limited | Extraterritorial | 2014 |
| Hillrigg Limited | Hospitality | 2007 |
| Holm Galloway Limited | Hospitality | 2024 |
| Horizon (Renovation) Limited | Construction | 2023 |
| Howden Property Limited | Real estate | 2015 |
| Hugh Gourlay Limited | Information & communication | 2011 |
| Human Resources Studio Ltd | Professional & scientific | 2019 |
| Hydro Energy Developments Limited | Professional & scientific | 2001 |
| I Shall Wear Purple Limited | Other services | 2007 |
| I.B. Aitchison & SON Limited | Construction | 2013 |
| Immersive Scotland Ltd | Arts & recreation | 2026 |
| Inclusive Training Ltd | Professional & scientific | 2018 |