| Elion Capital Limited | Financial services | 2025 |
| Elite Gym Hereford Limited | Arts & recreation | 2023 |
| Ellis Environmental Ltd | Administrative & support | 2018 |
| Ellis Passenger Transport Limited | Transport & logistics | 2015 |
| Elliston Developments Ltd | Construction | 2018 |
| Elliston Lettings Ltd | Real estate | 2017 |
| Elra Insure Ltd | Administrative & support | 2017 |
| EM & CM LLP | | 2018 |
| EMC Developments Limited | Real estate | 2006 |
| EMC Services (Wales) Limited | Construction | 2002 |
| Emily Enchanted Crystals Limited | Wholesale & retail | 2024 |
| Emma Porter Limited | Information & communication | 2017 |
| Empathyworks Community Interest Company | Professional & scientific | 2025 |
| Empathyworks Leadership Consultancy Ltd | Professional & scientific | 2025 |
| Emperor Coffee Ltd | Manufacturing | 2018 |
| Enabling Applications Ltd | Information & communication | 2008 |
| Endaf Davies SA Properties Ltd | Hospitality | 2025 |
| Engineering in Practice Solutions (Eips) Ltd | Professional & scientific | 2024 |
| Enhanced Group Limited | Manufacturing | 2018 |
| Enviroworxwales Limited | Construction | 2022 |
| Epla Group Ltd | Professional & scientific | 2023 |
| Epla Health Ltd | Professional & scientific | 2023 |
| Epla Retail Ltd | Wholesale & retail | 2023 |
| Equine Shop Porthyrhyd Limited | Wholesale & retail | 2011 |
| Equus Ferus International CIC | Agriculture & fishing | 2015 |
| Erch Ltd. | Hospitality | 2012 |
| Erica Malcolm Snts Ltd | Health & social care | 2010 |
| Erwain Escapes Limited | Real estate | 2022 |
| Escalwen Limited | Agriculture & fishing | 2012 |
| Esgairhir Uchaf Farm Ltd. | Agriculture & fishing | 2020 |
| Essential Management Consultants Ltd | Professional & scientific | 2016 |
| Essential Management Training Ltd | Education | 2010 |
| Essential Procurement Ltd | Professional & scientific | 2014 |
| Ethereal Productions Limited | Information & communication | 2017 |
| Eurobridge Trade PC | | 2018 |
| Euronet Distribution Limited | Wholesale & retail | 2005 |
| Europa Transport and Sons Limited | Transport & logistics | 2023 |
| European Clothing Company Ltd | Wholesale & retail | 2021 |
| European Homes Limited | Construction | 1999 |
| EV Life Ltd | Construction | 2019 |
| Evans and Jones Ltd | Wholesale & retail | 2012 |
| Evans Bros (Aberaeron) Limited | Real estate | 2014 |
| Evans Bros (Carmarthen) Limited | Real estate | 2014 |
| Evans Bros (Lampeter) Limited | Real estate | 2014 |
| Evans Bros (Llanybydder) Limited | Real estate | 2014 |
| Evans Bros Auctioneers & Estate Agents Limited | Real estate | 2004 |
| Evans Country Stores Limited | Wholesale & retail | 2015 |
| Evans Engineering Ltd | Professional & scientific | 2023 |
| Evans Estates Limited | Real estate | 2012 |
| Evans Plant Hire Limited | Administrative & support | 2023 |
| Evans Sons&Daughters Limited | Transport & logistics | 2024 |
| Evans’S Hospitality Limited | Hospitality | 2024 |
| Evince Aesthetics Limited | Health & social care | 2026 |
| Evolved Renovations Limited | Construction | 2002 |
| Exbond Services L.P. | | 2018 |
| Exburg Solutions L.P. | | 2018 |
| Excelsius Community Healthcare Limited | Health & social care | 2024 |
| Exeldent Cardiff Limited | Wholesale & retail | 2025 |
| Extreme Clean (Wales) Limited | Administrative & support | 2014 |
| Extremus Dog Training Ltd | Other services | 2013 |
| Extremus K9 Ltd | Agriculture & fishing | 2021 |
| Eynon's of ST Clears Limited | Real estate | 2002 |
| F a S Security Limited | Extraterritorial | 2007 |
| F.C.M.Limited | Other services | 1960 |
| F4G Ltd | Financial services | 2013 |
| Fabulicious Crafting Ltd | Wholesale & retail | 2025 |
| Face of Flowers Limited | Wholesale & retail | 2007 |
| Fairbrother Fabrication Limited | Manufacturing | 2026 |
| Fairwood Residential Estates Ltd | Professional & scientific | 2007 |
| Falcon Europe Ltd | Wholesale & retail | 2023 |
| Falcon Racing UK CIC | Arts & recreation | 2026 |
| Farm & Fell Ltd | Hospitality | 2021 |
| Farran Swann Limited | Information & communication | 2024 |
| Farsight Solutions Limited | Hospitality | 2013 |
| FBM Films Ltd | Information & communication | 2026 |
| Fearn Property Limited | Real estate | 2026 |
| Fecci's Ice Cream Limited | Manufacturing | 1994 |
| Felin Developments Limited | Construction | 2017 |
| Felinfach Leisure Limited | Hospitality | 2024 |
| Felton & Reed Ltd | Manufacturing | 2017 |
| Fembiome Ltd | Wholesale & retail | 2024 |
| Fferm Frongoch Fach Ltd | Agriculture & fishing | 2022 |
| Ffion Snell Tattoos Limited | Other services | 2026 |
| Fflur Dafydd CYF | Other services | 2015 |
| Fforest Edge Ltd | Agriculture & fishing | 2024 |
| Fforest Edge TIR Ltd | Real estate | 2026 |
| Ffoshelig Coaches Limited | Transport & logistics | 2013 |
| Fieldtofork Ltd | Hospitality | 2022 |
| Fight Project Gym Limited | Arts & recreation | 2023 |
| Film Photography Limited | Wholesale & retail | 2010 |
| Findmypt Limited | Arts & recreation | 2014 |
| Finklecatering Ltd | Hospitality | 2022 |
| Finklecottages Ltd | Real estate | 2022 |
| Finkledrinks Ltd | Hospitality | 2022 |
| Finklespirits Ltd | Hospitality | 2022 |
| Fiona Cardis Ltd | Wholesale & retail | 2015 |
| Fire Assessed Ltd | Public administration | 2021 |
| Firecare Alarm Systems Limited | Public administration | 2003 |
| First Reflections Cleaning Contractors Limited | Administrative & support | 2015 |
| First Sentry Security Limited | Administrative & support | 2012 |