| Emswaste Limited | Water & waste | 2022 |
| Emtech Security Ltd | Construction | 2025 |
| Emtu Global Ltd | Wholesale & retail | 2025 |
| Emurrsamm Ltd | Wholesale & retail | 2020 |
| Emvaria Ltd | Wholesale & retail | 2025 |
| EMW & Training Ltd | Health & social care | 2013 |
| EMW Consulting Ltd | Information & communication | 2025 |
| EMY Media Ltd | Administrative & support | 2023 |
| EMY Phillip Interiors Ltd | Wholesale & retail | 2019 |
| Emyco Ltd | Wholesale & retail | 2025 |
| Emynor Global Ltd | Wholesale & retail | 2025 |
| Emyr Locums Limited | Health & social care | 2026 |
| Emyr Pierce Solicitors Limited | Professional & scientific | 2020 |
| Emyr-Walters Radiology Limited | Health & social care | 2022 |
| Emyrox Ltd | Construction | 2025 |
| Emzawi FIC Ltd | Financial services | 2025 |
| Emzed Capital Ltd | Professional & scientific | 2025 |
| EN Maison Property Investment Ltd | Real estate | 2020 |
| EN Property Limited | Real estate | 2014 |
| EN Timber 4 UK Ltd | Manufacturing | 2019 |
| EN Vogue Limited | Wholesale & retail | 2024 |
| Enaaya Ltd | Wholesale & retail | 2018 |
| Enabling Talent Ltd | Professional & scientific | 2018 |
| Enare Electrical Contractors Limited | Construction | 2024 |
| Enbsi Ltd | Other services | 2025 |
| ENC4U Limited | Information & communication | 2014 |
| Encephalon-It Ltd | Information & communication | 2017 |
| Enchen (UK) Limited | Wholesale & retail | 2025 |
| Encidu Group Ltd | Information & communication | 2025 |
| Encina Holdings Ltd | Financial services | 2017 |
| Enclave Consulting Limited | Information & communication | 2024 |
| Encode Marketing Limited | Professional & scientific | 2019 |
| Encom Business Automation Ltd | Information & communication | 2019 |
| Encon Construction Limited | Construction | 2003 |
| Encore Capital Group Inc Ltd | Other services | 2021 |
| Encore Gaming Ltd | Manufacturing | 2023 |
| Encore Ltd | Arts & recreation | 2023 |
| END Squared Ltd | Information & communication | 2018 |
| Endeavor Media Limited | Manufacturing | 2016 |
| Endeavor Partners Ltd | Professional & scientific | 2023 |
| Endecotts Property Solutions Ltd | Construction | 2010 |
| Endings Jewels Limited | Wholesale & retail | 2020 |
| Endless E-Commerce Limited | Wholesale & retail | 2021 |
| Endlessloveboutique Ltd | Wholesale & retail | 2025 |
| Endolevicus Health Solutions Limited | Health & social care | 2013 |
| ENE Leader Ltd | Construction | 2018 |
| Eneed Limited | Wholesale & retail | 2021 |
| Enemesi Limited | Professional & scientific | 2025 |
| Enenesis Ltd | Transport & logistics | 2014 |
| Enerco Steel Trade Limited | Wholesale & retail | 2005 |
| Energeia Medical Limited | Health & social care | 2023 |
| Energeia Resources Limited | Information & communication | 2005 |
| Energen Electrical Ltd | Construction | 2018 |
| Energen Solar Ltd | Construction | 2020 |
| Energieausweise Ltd | Wholesale & retail | 2025 |
| Energreen Holding Corp Ltd | Financial services | 2023 |
| Energy (PHT GP) Limited | Mining & quarrying | 2005 |
| Energy 4 Life Enterprise Ltd | Energy | 2020 |
| Energy Assessors (Wales) Limited | Professional & scientific | 2007 |
| Energy Blue Ltd | Transport & logistics | 2017 |
| Energy Direct Consultants Limited | Energy | 2017 |
| Energy Exchange (GB) Limited | Professional & scientific | 2001 |
| Energy Genie Ltd | Professional & scientific | 2017 |
| Energy Monitoring Operations Ltd | Administrative & support | 2018 |
| Energy Rated Limited | Manufacturing | 2025 |
| Energy River International Trading Co., Ltd | Wholesale & retail | 2024 |
| Energy Sanctuary Ltd | Energy | 2011 |
| Energy SHY Ltd | Energy | 2018 |
| Energy Solutions (ES) Ltd | Administrative & support | 2016 |
| Enerheat Limited | Construction | 2024 |
| Enet Sameday Ltd | Other services | 2020 |
| Eneta Marketing PC | | 2017 |
| Enfys Developments Limited | Construction | 2012 |
| Enfys Hapus Nursery and Day Care Centre Limited | Education | 2017 |
| Enfys Homes Limited | Construction | 2018 |
| Enfys Services Ltd | Professional & scientific | 2023 |
| ENG2SEA Ltd | Professional & scientific | 2013 |
| Engage Sports Management Limited | Professional & scientific | 2012 |
| Engagesport LLP | | 2012 |
| Engagesport Services Limited | Extraterritorial | 2012 |
| Engcom Services Limited | Professional & scientific | 2005 |
| Engelus Network Ltd | Professional & scientific | 2020 |
| Engenerate Limited | Administrative & support | 2016 |
| Engi Service Ltd | Other services | 2019 |
| Engi.Ai Limited | Information & communication | 2022 |
| Engic Ltd | Professional & scientific | 2019 |
| Engine Engineers UK Ltd | Wholesale & retail | 2023 |
| Engine Gaming Studio Limited | Information & communication | 2025 |
| Engine Power Technology Limited | Information & communication | 2021 |
| Enginecentre.Com Ltd | Wholesale & retail | 2024 |
| Engineered Performance Coatings Holding Ltd | Administrative & support | 2019 |
| Engineering & Management Service Ltd | Professional & scientific | 2019 |
| Engineering Dimensions Management Resources Limited | | 1986 |
| Engineering Fabrication Limited | Manufacturing | 1999 |
| Engineering Fmcg Services Ltd | Professional & scientific | 2019 |
| Engineering Projects & Management Services Ltd | Professional & scientific | 2017 |
| Engineers for Overseas Development Limited | Construction | 2011 |
| Engineers MRO Products Ltd | Professional & scientific | 2021 |
| ENGINEERSM8 Limited | Manufacturing | 2025 |
| English Made Easy Ltd | Education | 2021 |